Structural characterization and hypoglycemic activity of Trichosanthes peel polysaccharide

Chen, Tianle; Zhang, Min; Li, Jinglei; Surhio, Maheen Mahwish; Li, Beibei; Ye, Ming

doi:10.1016/j.lwt.2016.02.024

Cited by 58 publications

(25 citation statements)

References 34 publications

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“…A number of studies reported that some plant polysaccharides exhibit hypoglycaemic activities; these plant polysaccharides include those derived from Trichosanthes peel, bamboo shoots (Leleba oldhami Nakal) shells, Lycium barbarum L, loach, Grifola frondosa and Moringa oleifera Lam. leaves (Chen et al 2016(Chen et al , 2017Huang et al 2015;Tang et al 2015;Xiao et al 2015;Zhou et al 2015;Zheng et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Hypolipidaemic and hypoglycaemic properties of pumpkin polysaccharides

Song

Sun

2017

3 Biotech

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Both hyperglycaemia and hyperlipidaemia are major risk factors for the development of coronary artery diseases and atherosclerosis, and therefore therapeutic drugs must be developed for treatment of them. Pumpkin polysaccharides (PPs) are biomacromolecules with varying bioactivities. In this study, PPs were extracted with commercial thermostable α-amylase, and their hypolipidaemic and hypoglycaemic activities were evaluated. Twenty four KKAy mice were divided into two groups: control was fed with high-fat diet; while the PP group was fed with high-fat diet with the addition of PPs at the same time, for 6 weeks. PP diet reduced body weight gain, the levels of plasma insulin, serum triglyceride, cholesterol, low-density lipoprotein cholesterol and blasting blood glucose in mice and improved the level of high-density lipoprotein cholesterol and liver glycogen. Results indicate that PPs had high hypolipidaemic and hypoglycaemic activities and could be used as potential drugs for treatment of hyperlipidaemia and hyperglycaemia.

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Section: Introductionmentioning

confidence: 99%

Hypolipidaemic and hypoglycaemic properties of pumpkin polysaccharides

Song

Sun

2017

3 Biotech

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“…The absorption peak at 1036 cm −1 was the characteristic peak of pyranoside and the weak small peak at 904 cm −1 indicated β -glucosidic bonds in TFPI [21]. All the structural characteristics of TFPI were quite different from the structure of TPP-1 previously reported by Chen et al [2]. Such a result might be due to the different sources of T. kirilowii Maxim and also the different extraction methods.…”

Section: Resultsmentioning

confidence: 99%

“…The weaker absorption peak at 2931 cm −1 was mainly contributed by the C-H (-CH 2 -, -CH 3 , and -CHOH) stretching vibration [2]. The absorption peak at 1643 cm −1 was caused by the bending mode of bound water [19], and the absorption peak at 1438 cm −1 was C-O stretching vibration [20].…”

Section: Resultsmentioning

confidence: 99%

Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

Chen

Shen

et al. 2017

BioMed Research International

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An efficient procedure for ultrasound-assisted enzymatic extraction of crude polysaccharides from Trichosanthes Fructus (crude TFP) using response surface methodology (RSM) was developed. The Box–Behnken design was applied to optimize the effects of pH (X1), enzyme amount (X2), extraction temperature (X3), and liquid-to-solid ratio (X4) on the extraction. The statistical analysis indicated that the independent variables (X4, X2, and X3), the quadratic coefficients (X12, X22, X32, and X42), and the interaction coefficient (X1X3) had significant impact on the yield of crude TFP. The optimal conditions were determined as follows: pH 4.5, enzyme amount 5000 u/g, extraction temperature 45°C, and liquid-to-solid ratio 30 ml/g. The experimental yield of crude TFP was 6.58%, which was very close to the predicted yield of 6.71%. TFPI was then purified and characterized with Sephadex G-100 column, UV-Vis, GPC, and FT-IR. The average molecular weight of TFPI was calculated to be 1.49 × 105 Da. TFPI exhibited strong reducing power and possessed not only remarkable scavenging activities against ABTS•+ and DPPH radicals, but also high antitumor activities in C4-2, DU145, and PC3 cells. The results suggest that Trichosanthes Fructus and TFPI could be a novel potent natural medicine with antioxidant and antitumor activities.

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“…[13] Analysis of monosaccharide composition Five mg SHDPs were hydrolysed with TFA by Wang's method. [14] The hydrolysate was treated successively with 30 mg NaBH 4 for 3 h. After neutralization with acetic acid, 3 mL acetic anhydride and 3 mL pyridine were added into the residue at 100°C and reacted for 1 h. The alditol acetate derivatives were analysed by gas chromatography-mass spectrometry (GC-MS; Shimadzu Co. Japan) according to the method of Chen et al [15] The monosaccharide standards were detected by the same methods. The monosaccharides were identified by their retention times (Rt) and quantitatively determined by their peak areas.…”

Section: Analysis Of Chemical Compositionmentioning

confidence: 99%

“…Valle & Hoch method. The assay mixture consisted of 1.5 mL sodium pyrophosphate buffer of pH 8.8, 0.5 mL of oxidized coenzyme Ι (NAD + , 0.027 mol/L), 0.5 mL ethanol of 11.5%v/v(8.85 mL of distilled water mixed with 1.15 mL anhydrous ethanol), and the reaction was started immediately by adding 0.1 mL of different concentrations of SHDPs(5,10,15,20, and 25 mg/mL) in tubes and sealed. After shaking vigorously, the mixture was incubated at 25°C in a water bath for 5 min to achieve temperature equilibrium.…”

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confidence: 99%

Characterization and bioactivities of polysaccharide from spentHovenia dulcispeduncles by alkali pretreatment

Liu

Cai

Qiang

2017

International Journal of Food Properties

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A novel heteropolysaccharide named SHDP was isolated from spent Hovenia dulcis peduncles (SHD) using alkali extraction. The preliminary characterization of the polysaccharide was performed by physicochemical and spectral methods. The bioactivities in vitro were also investigated. The results indicated that SHDPs are homogeneous with an average molecular weight of approximately 4570 kDa, and contain uronic acid (2.3%) and protein (3.6%). It is mainly composed of rhamnose, fucose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 3.

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Structural characterization and hypoglycemic activity of Trichosanthes peel polysaccharide

Cited by 58 publications

References 34 publications

Hypolipidaemic and hypoglycaemic properties of pumpkin polysaccharides

Hypolipidaemic and hypoglycaemic properties of pumpkin polysaccharides

Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

Characterization and bioactivities of polysaccharide from spentHovenia dulcispeduncles by alkali pretreatment

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