Polysaccharides from<i>Trichosanthes Fructus</i>via Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

Chen, Fujia; Li, Dahong; Shen, Hongqi; Wang, Chunhong; Li, Enzhong; Huang, Xing; Zhao, Qingchun; Shi, Juan; Nguyen, Hoang Chinh; Liu, Jiayang

doi:10.1155/2017/6160785

Cited by 15 publications

(17 citation statements)

References 33 publications

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“…Chen et al. (2017) successfully reported the polysacchardies from Trichosanthes Fructus using UAE and optimized the extraction parameters using RSM for maximum yield of polysaccharides. Polysaccharides were extracted by ultrasound‐assisted method from hazelnut skin and tobacco leaves.…”

Section: Resultsmentioning

confidence: 99%

Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Zhang

et al. 2021

Journal of Food Science

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In this study, response surface methodology was employed to optimize the ultrasound‐assisted extraction (UAE) process of perilla seed meal polysaccharides (PSMP). The optimal conditions for UAE of PSMP were: liquid–solid ratio of 26.00 mL/g, ultrasonic temperature of 43.00 °C, ultrasonic time of 52.00 min, and ultrasonic power of 229.00 W, the optimal conditions lead to an yield of 6.137 ± 0.062%. The structural characteristics of molecular weight, compositional monosaccharides, and glycosidic linkages were determined by size exclusion chromatography with multiangle light scattering, gas chromatography‐mass spectrometry, Fourier‐transfer infrared spectroscopy, and nuclear magnetic resonance detections. Scanning electron microscopy analysis showed that many holes were formed on the surface of PSM after UAE. The antioxidant activities of PSMP were investigated using various assays in vitro. The results suggested that PSMP is potential natural resource of antioxidants for medicine and functional foods. Practical Application The selection of raw material perilla seed meal is conducive to the comprehensive utilization of edible resources. With consumer demands for newly developed foods with natural, wholesome ingredients are increasing nowadays. This study provides effective reference for in‐depth research on other medicine‐food dual‐use resources. Ultrasound‐assisted extraction (UAE) is a promising alternative method for hot water reflux extraction (HWRE) of polysaccharides for advantages of high efficiency and energy saving. In this work, the UAE process optimized by response surface methodology is more suitable for industrial application that can effectively decrease total cost of production by reducing the extraction temperature, shortening extraction time, and increasing raw material utilization.

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Section: Resultsmentioning

confidence: 99%

Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Zhang

et al. 2021

Journal of Food Science

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“…Our results showed that EGLP exhibits better antitumor effect than the original polysaccharide, implying the potential of EGLP in improving quality of life of the organism as well as inhibiting tumor growth, which was in accordance withthe previous research indicating that the enzymatic extraction of crude polysaccharide from Trichosanthes fructus exerts higher antitumor activity than the native polysaccharide. 8…”

Section: Discussionmentioning

confidence: 99%

Antitumor activity of enzymatically hydrolyzed Ganoderma lucidum polysaccharide on U14 cervical carcinoma–bearing mice

Kong

Yao

Zhang

2019

Int J Immunopathol Pharmacol

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Polysaccharides from Ganoderma lucidum have been demonstrated to possess diverse biological activities. Despite lots of studies on the biological activities of Ganoderma lucidum polysaccharide (GLP), little is known regarding the medicinal potential of low–molecular weight enzymatically hydrolyzed Ganoderma lucidum polysaccharide (EGLP). EGLP was prepared by enzymatic degradation and its potential effects in U14 cervical tumor–bearing mice were evaluated. Both GLP and EGLP delayed tumor growth of the tumor xenograft. The EGLP was superior to native polysaccharide. Moreover, EGLP treatment could effectively protect the immune organs of U14 cervical carcinoma–bearing mice. In addition, the EGLP treatment ameliorated oxidative stress as compared with cyclophosphamide (CTX). Compared with the MC group, the expression of Bcl-2 and COX-2 was obviously decreased by EGLP treatment, whereas the expression of Bax and cleaved caspase-3 was obviously increased. These results indicated that EGLP showed stronger antitumor activity with lower toxic effects and had the potential to be a novel antitumor agent.

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“…ultrasound irradiation, 50°C, 100 W, 60% duty cycle, and solid to solvent ratio of 0.3:20) [28]. Polysaccharides from T. fructus were obtained using UAE by optimizing several independent variables and their interactions via response surface methodology [29]. Researchers have extracted polyphenols, flavonoids, polysaccharides and anthocyanins from different crop varieties like mulberry, blackcurrant, peanut shells, etc.…”

Section: Cactusmentioning

confidence: 99%

Improvements in the extraction of bioactive compounds by enzymes

Marathe

Jadhav

Bankar

et al. 2019

Current Opinion in Food Science

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Bioactives from plants are always in high demand in nutraceutical, pharmaceutical and functional food sectors due to their health benefits. This intensifies the need of extraction of bioactives by different methods that can improve the yield and purity of the compound. Enzyme helps in the release of bioactives from the plant material under optimized conditions so as to make the extraction process efficient. Though enzymatic extraction of bioactive has been used since long, it needs improvement to further enhance the yield, reduce the process time and to make the process cost competitive. The combination of enzymatic extraction with other green techniques such as ultrasound extraction, supercritical fluid extraction, three phase partitioning, ionic liquid extraction and microwave extraction can boost the advantages of enzymatic extraction. This review focuses on the improvement in the enzymatic extraction techniques for the bioactive in detail. Keywords Bioactives Enzyme-assisted extraction (EAE) Ultrasound assisted enzyme extraction (UAEE) Enzyme-assisted supercritical extraction (EASCFE) Ionic liquids enzyme assisted extraction (ILEAE) Microwave assisted enzymatic extraction (MAEE) Enzyme assisted three phase partition (EATPP) High pressure assisted enzymatic extraction (HPAEE)

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Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

Cited by 15 publications

References 33 publications

Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Optimization of ultrasound‐assisted extraction of polysaccharides from perilla seed meal by response surface methodology: Characterization and in vitro antioxidant activities

Antitumor activity of enzymatically hydrolyzed Ganoderma lucidum polysaccharide on U14 cervical carcinoma–bearing mice

Improvements in the extraction of bioactive compounds by enzymes

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