Structural characteristics of the antigenic capsular polysaccharides and lipopolysaccharides involved in the serological classification of Actinobacillus (Haemophilus) pleuropneumoniae strains

Perry, Malcolm B.; Altman, Eleonora; Brisson, Jean‐Robert; Beynon, Linda M.; Richards, James C.

doi:10.1016/0888-0786(90)90018-j

Cited by 134 publications

(138 citation statements)

References 33 publications

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“…It is unlikely that cross-reactions in the serological tests account for the positive associations, since the ELISA tests used have a high specificity. Cross-reactions are more common between serovars 1, 9 and 11; serovars 3, 6 and 8, and serovars 4 and 7 because of structural similarities in some epitopes of the lipopolysaccharides in these serovars [26]. The positive associations may result from similar management practices or environmental conditions promoting the spread of these serovars.…”

Section: Discussionmentioning

confidence: 99%

Herd factors associated with the seroprevalences of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from farrow-to-finish pig herds

Maes¹,

Chiers

Haesebrouck

et al. 2001

Vet. Res.

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-This cross-sectional epidemiologic study was conducted in 150 randomly selected farrowto-finish pig herds to investigate descriptive epidemiological characteristics of infections with three different serovars of Actinobacillus pleuropneumoniae, and to identify risk factors for the within-herd seroprevalences of these serovars. Different farm characteristics (n = 28) were examined as potential risk factors for the percentage of pigs with antibodies against serovars 2, 3 and 9. The presence of antibodies was measured using an indirect ELISA. Logistic regression analyses were used to assess the associations between the potential risk factors and the proportion of seropositive pigs. The median within-herd seroprevalences were 95% (range: 0-100%), 100% (range: 10-100%), and 35% (range: 0-100%) for serovars 2, 3, and 9, respectively. There was a positive association (P < 0.001) between each of these serovars. The within-herd seroprevalence of serovar 2 was significantly higher in farms that purchased gilts from ≥ 2 origin herds (OR = 2.33; P < 0.05) and in farms with poor biosecurity measures (OR = 4.62; P < 0.05). The proportion of pigs seropositive for serovar 3 was significantly higher when tested pigs were slaughtered in May-August and in November-December (OR = 5.96; P < 0.001), in herds without a growing unit (OR = 2.63; P < 0.01), and in herds with a direct air-entry into the finishing unit (OR = 1.92; P < 0.05). The within-herd seroprevalence of serovar 9 increased significantly in herds with poor biosecurity measures (OR = 1.76; P < 0.05). The study documented that infections with A. pleuropneumoniae serovars 2, 3, and 9 were very common in the selected herds, and that the sero-epidemiological characteristics and risk factors showed some variation depending on the serovar. The purchase policy of gilts and biosecurity measures are risk factors that can be improved fairly easily on pig farms. swine / Actinobacillus pleuropneumoniae / seroprevalence / epidemiology Vet. Res. 32 (2001) 409-419 409

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Section: Discussionmentioning

confidence: 99%

Herd factors associated with the seroprevalences of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from farrow-to-finish pig herds

Maes¹,

Chiers

Haesebrouck

et al. 2001

Vet. Res.

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“…This cross-reactivity was attributed to common polysaccharide antigens by Mittal (24), who also concluded that these serotypes do not share CPS epitopes but that the crossreactivity observed may be due to somatic antigens. Analyses of the CPS of serotypes 1 and 9 carried out in our laboratory demonstrated that their structures, while having some similarities, contained unique epitopes (27).…”

mentioning

confidence: 90%

Characterization of the lipopolysaccharide O antigens of Actinobacillus pleuropneumoniae serotypes 9 and 11: antigenic relationships among serotypes 9, 11, and 1

et al. 1992

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The antigenic lipopolysaccharide 0 polysaccharides of capsular serotypes 9 and 11 were examined by chemical, immunological, and nuclear magnetic resonance methods. Immunodiffusion tests carried out on these 0 antigens indicated that both contained common epitopes which were also shared by Actinobacilus peuropneumonae serotype 1. Chemical analysis and high-field nuclear magnetic resonance spectroscopy showed that the 0 antigens of serotypes 9 and 11 were high-molecular-weight polymers consisting of a backbone of repeating trisaccharide units composed of a-L-rhamnopyranosyl and a-D-glucopyranosyl residues (2:1). One of the c-L-rhamnose units forms a branch point and is stoichiometrically substituted with terminal 2-acetamido-2-deoxy-1-D-glucose residues in the serotype 11 0 polysaccharide, but only to the extent of 25% in the serotype 9 0 polysaccharide. Thus, the serotype 9 0 polysaccharide contains two different repeating units: a tetrasaccharide unit with the same structure as that of the serotype 11 0 polysaccharide and a trisaccharide unit:where R = j3-D-GlcpNAc for serotype 1 and 11 0 polysaccharides, and R = H (75%) and R = f-D-GlcPNAc (25%o) for serotype 9. The structure of the previously determined serotype 1 0 polysaccharide (E. Altman, J.-R Brisson, and M. B. Perry, Biochem. Cell. Biol. 64:17-25, 1986) is identical to that of the serotype 11 0 polysaccharide. We propose a more complete serotyping scheme for A. pleuropneumoniae which includes designation of both the capsular (K) and 0 antigens. Actinobacillus pleuropneumoniae is a gram-negative, pleomorphic rod which causes a highly contagious disease in pigs. The occurrence of A. pleuropneumoniae-associated pleuropneumonia has increased since 1976 (29) because of intensified pig production, and A. pleuropneumoniae lipopolysaccharide (LPS) is recognized as an important virulence factor (30). In attempts to combat the disease, serological methods of identification of infected animals based on the identification and characterization of serotype-specific antigens would greatly assist in solving the problem. Information on the structures of the LPS 0 antigens of A. pleuropneumoniae involved as virulence factors, in protection, and in serodiagnosis could provide data leading to the rational development of suitable, protective vaccines and the production of specific serotyping and serodiagnostic reagents.Currently, 12 serotypes ofA. pleuropneumoniae based on capsular polysaccharides (CPS) are recognized (26). Capsular serotypes 1, 5, and 7 are the predominant disease isolates from A. pleuropneumoniae outbreaks in the United States and Canada (28), whereas serotype 9 is predominant in The Netherlands (19). Serological cross-reactivity between A. pleuropneumoniae serotypes 1 and 9 has been detected by * Corresponding author. t NRCC no. 34260. complement fixation and gel diffusion methods (25). This cross-reactivity was attributed to common polysaccharide antigens by Mittal (24), who also concluded that these serotypes do not share CPS epitopes but that the cross...

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“…pleuropneumoniae serovars 4 and 7 have similar O-side chains in their LPS, 16 which can cause serologic cross-reactivity between these serovars. This explains the reactivity of sera from pigs experimentally infected with A. pleuropneumoniae serovar 4 in the A. pleuropneumoniae serovar 7 ELISA.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that capsular polysaccharides of serovar 15 share common substructurel elements with serovar 7 and serovar 12. 16 The ELISA in this study was based on long chain LPS purified from serovar 7 and showed no cross-reaction with serovar 15.…”

Section: Discussionmentioning

confidence: 99%

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An Indirect Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Actinobacillus Pleuropneumoniae Serovar 7 in Pig Serum

et al. 2007

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Abstract. Lipopolysaccharide (LPS) antigen was purified from Actinobacillus pleuropneumoniae serovar 7 by phenol-water extraction and fractionated on a, S-100 Sephacryl column. High molecular weight fractions of LPS purified from the S-100 column were pooled and used as antigen in an indirect serovar 7 ELISA. The ELISA was evaluated with sera from pigs experimentally infected with 11 different A. pleuropneumoniae serovars of biotype 1. Estimation of sensitivity and specificity of the A. pleuropneumoniae serovar 7 ELISA was performed using pig sera from herds naturally infected with A. pleuropneumoniae serovar 7 as well as sera from herds free of infection with A. pleuropneumoniae serovar 7. When compared to the complement fixation test (CFT) as a reference test, the ELISA showed much higher sensitivity and statistically equivalent specificity.

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Structural characteristics of the antigenic capsular polysaccharides and lipopolysaccharides involved in the serological classification of Actinobacillus (Haemophilus) pleuropneumoniae strains

Cited by 134 publications

References 33 publications

Herd factors associated with the seroprevalences of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from farrow-to-finish pig herds

Herd factors associated with the seroprevalences of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from farrow-to-finish pig herds

Characterization of the lipopolysaccharide O antigens of Actinobacillus pleuropneumoniae serotypes 9 and 11: antigenic relationships among serotypes 9, 11, and 1

An Indirect Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Actinobacillus Pleuropneumoniae Serovar 7 in Pig Serum

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