An Indirect Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to <i>Actinobacillus Pleuropneumoniae</i> Serovar 7 in Pig Serum

Klausen, Joan; Ekeroth, Lars; Grøndahl-Hansen, J.; Andresen, Lars Ole

doi:10.1177/104063870701900303

Cited by 17 publications

(13 citation statements)

References 14 publications

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“…The England and Wales serovar profile contrasts with that found in other European countries. For example, serovar 9/11 predominates in the Czech Republic ( Kucerova and others 2005 ) and serovar 2 in Denmark ( Klausen and others 2007 ), Norway and Sweden ( Gottschalk 2012 ). No serovar 3 isolates were found, possibly reflecting the known low virulence of this serotype ( Rosendal and others 1985 ), and that the A. pleuropneumoniae isolates evaluated were from clinically confirmed cases.…”

mentioning

confidence: 99%

Actinobacillus pleuropneumoniae serovar 8 predominates in England and Wales

Bossé

Williamson

et al. 2016

Veterinary Record

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mentioning

confidence: 99%

Actinobacillus pleuropneumoniae serovar 8 predominates in England and Wales

Bossé

Williamson

et al. 2016

Veterinary Record

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“…The plates for these 2 assays were coated with LPS of certain serovars. 24 A total of 20 μl of each serum sample was used and diluted 1:200. The quad-plate ELISA-1 results were expressed as corrected sample-to-positive (S/P) ratio.…”

Section: Serological Assaysmentioning

confidence: 99%

Evaluation of diagnostic assays for the serological detection ofActinobacillus pleuropneumoniaeon samples of known or unknown exposure

et al. 2013

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Abstract. Accurate diagnosis of exposure to Actinobacillus pleuropneumoniae is important for maintaining negative farms. In the present study, the ability of a dual-plate complement fixation (CF) assay and 3 commercially available enzymelinked immunosorbent assays (ELISAs; quad-plate ELISA-1, single-plate ELISA-2, and single-plate ELISA-3) in detecting serological evidence of A. pleuropneumoniae exposure was compared using serum samples of experimentally infected or vaccinated pigs, or field samples from the United States. Forty-two pigs were divided into groups of 2 pigs and were inoculated with 1 of 15 A. pleuropneumoniae strains representing all known serovars of A. pleuropneumoniae, or with Actinobacillus suis, or were vaccinated with a bacterin containing A. pleuropneumoniae serovar 1, 3, 5, or 7. Serum samples collected at the day of inoculation or vaccination and 7, 14, 21, and 28 days later were used to compare the assays. On samples from experimentally infected pigs, the dual-plate CF assay, quad-plate ELISA-1, single-plate ELISA-2, and single-plate ELISA-3 had sensitivities of 0.46, 0.74, 0.13, and 0.13 and specificities of 0.90, 1.0, 1.0, and 1.0, respectively. Vaccinated pigs were identified only by the dual-plate CF assay and the quad-plate ELISA-1. In addition, 90 serum samples with unknown A. pleuropneumoniae exposure collected under field conditions were tested with all assays. The agreement of the 4 assays on field samples was slight to fair. While several assays are available for demonstration of A. pleuropneumoniae exposure, differences in assay targets complicate test choices. Decisions on which assay or combination of assays to use depend on the specific reasons for running the assays.

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“…The prevalence of particular serotypes is usually studied either by determining the presence in pigs of antibodies to particular serotypes or by examining bacterial isolates directly. Several methods are used to detect the antibodies, including elisa s, in which the capture antigen can be lipopolysaccharide, capsule or mixed extracts (Bossé and others 1993, Rodriguez-Barbosa and others 1996, Lacouture and others 1997, Lebrun and others 1999, Inzana and Fenwick 2001, Grøndahl-Hansen and others 2003), or complement fixation tests (Sidibe and others 1993, Andreasen and others 2000, Enoe and others 2001, Klausen and others 2007). Methods applying serotyping directly to bacterial isolates are widely used and can give valuable information (Lo 1997, Dubreuil and others 2000).…”

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confidence: 99%

pcr specific for Actinobacillus pleuropneumoniae serotype 3

Zhou¹,

Jones²,

Angen

et al. 2008

Veterinary Record

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Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific pcr, based on the capsule locus, which can be used in a multiplex format with the organism's specific gene apxIV. The pcr test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.

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An Indirect Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Actinobacillus Pleuropneumoniae Serovar 7 in Pig Serum

Cited by 17 publications

References 14 publications

Actinobacillus pleuropneumoniae serovar 8 predominates in England and Wales

Actinobacillus pleuropneumoniae serovar 8 predominates in England and Wales

Evaluation of diagnostic assays for the serological detection ofActinobacillus pleuropneumoniaeon samples of known or unknown exposure

pcr specific for Actinobacillus pleuropneumoniae serotype 3

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