Structural and ultrastructural differences between field, micropropagated and acclimated leaves and stems of two Leucospermum cultivars (Proteaceae)

Suárez, Ernesto; Alfayate, Carmen; Pérez-Francés, J.F.; Rodríguez-Pérez, J.A.

doi:10.1007/s11240-018-1487-5

Cited by 6 publications

(2 citation statements)

References 48 publications

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“…The abaxial leaf surface of ex vitro-acclimatized plantlets revealed a relaxed surfaces with many well-defined stomata (Figure 4a2), with a homogenous aperture, and functional guard cells with open and closed stomata (Figure 4b2). Similar results were obtained in Ceratonia siliqua [60] and Leucospermum cultivars [61]. (GGGGT) 3 G 3…”

Section: Ultra-structural Difference Between In Vitro and Acclimatize...supporting

confidence: 82%

High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

et al. 2021

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Diplocyclos palmatus (L.) C. Jeffrey, commonly referred to as “Shivalingi” or “Lollipop climber” is a valuable medicinal plant with a climbing growth habit used in traditional medicine. It is reputed to have antiarthritic, anti-diabetic properties and to be useful in various skin and reproductive problems. Overexploitation of wild plants and low seed germination have resulted in the decline of the species in the wild. Thus, the present investigation was aimed to establish an effective in vitro propagation procedure for its large-scale production and conservation. Nodal explants, obtained from an established mother plant were grown on MS basal medium augmented with various cytokinins, alone or in combination with auxins, to study the morphogenic response. A maximum of 8.3 shoots/explants with an average shoot length of 7.2 cm were produced after six weeks on MS containing benzylaminopurine 5.0 µM + 1-naphthaleneacetic acid 2.0 µM. After 4 weeks of transfer, microshoots rooted well on a low nutrient medium of ½ MS + 1.0 µM indole-3-butyric acid, with a maximum of 11.0 roots/microshoot and an average root length of 7.4 cm. With an 80% survival rate, the regenerated plantlets were effectively acclimatized to natural conditions. DNA-based molecular markers were used to investigate the genetic uniformity. Scanning Electron Microscopic examination of leaves indicated the adaptation of the plantlets to natural, as evidenced by the formation of normal stomata. Gas chromatography-mass spectrometry analyses of mother and micropropagated plants were performed to identify essential secondary metabolites. The results obtained show that the in vitro propagation system can be adopted for preservation, large-scale production and secondary metabolites’ production in D. palmatus.

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Section: Ultra-structural Difference Between In Vitro and Acclimatize...supporting

confidence: 82%

High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

et al. 2021

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“…In our opinion, that structure was probably a consequence of the specific in vitro conditions. Moreover, similar morpho-and histological variations, such as a thin palisade layer and dense spongy parenchyma, have been observed in some other species propagated in vitro, which was an adverse effect of the high sugar content and addition of plant growth regulators in the medium [Sáez et al 2012, Suárez et al 2019.…”

Section: Discussionsupporting

confidence: 59%

Comparative leaf structural analysis of Nepeta nuda l. Plantlets, regenerated from cryopreserved shoot meristem and ex vitro-adapted plants

Stefanova,

Ganeva,

Koleva

2023

Acta Sci. Pol. Hortorum Cultus

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The leaf anatomy and chloroplast ultrastructure of Nepeta nuda L. plantlets regenerated in vitro from cryopreserved shoot apical meristem and in vitro-micropropagated plantlets were studied comparatively to assess whether cryoprocedure affected leaf morphogenesis. Both postcryo and in vitro plantlets failed to develop a distinguishable palisade layer, making the mesophyll appear homogeneous. Significant damage to the chloroplast envelope and substantial thylakoid ruptures were also observed. We assumed that the specific in vitro conditions more likely affected the structures than the cryotreatment itself. Light and transmission electron microscopy observations were also carried out on newly formed leaves of ex vitro-adapted plants. The examined leaf features were similar to those in the in situ plants – bifacial leaf lamina, double-layered palisade parenchyma, loosely arranged spongy parenchyma cells, and chloroplasts with intact envelope and evenly distributed throughout the stroma internal membrane system. The obtained histological and ultrastructural results revealed the retained morphogenetic potential of N. nuda plants and proved cryopreservation as a suitable method for long-term storage.

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Effect of forcing solutions used to break the seasonal influence on in vitro axillary bud sprouting of two Leucospermum (R. Br.) cultivars

Suárez

Alfayate

Rodríguez-Pérez

et al. 2021

Plant Cell Tiss Organ Cult

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Structural and ultrastructural differences between field, micropropagated and acclimated leaves and stems of two Leucospermum cultivars (Proteaceae)

Cited by 6 publications

References 48 publications

High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

Comparative leaf structural analysis of Nepeta nuda l. Plantlets, regenerated from cryopreserved shoot meristem and ex vitro-adapted plants

Effect of forcing solutions used to break the seasonal influence on in vitro axillary bud sprouting of two Leucospermum (R. Br.) cultivars

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