Effect of forcing solutions used to break the seasonal influence on in vitro axillary bud sprouting of two Leucospermum (R. Br.) cultivars

Suárez, Ernesto; Alfayate, Carmen; Rodríguez-Pérez, J.A.; Pérez-Francés, J.F.

doi:10.1007/s11240-021-02099-y

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…In other crops such as cassava (Manihot esculenta Crantz) [9], banana (Musa nana Lour.) [10], poplar (Populus L.) [11], ginkgo (Ginkgo biloba L.) [12], and (Primula × pubescens) [13], an AB-based tissue culture system is a viable method for seedling breeding. Therefore, we must study the potential of Hevea brasiliensis ABs for the seedling regeneration system through SE.…”

Section: Introductionmentioning

confidence: 99%

Induction of Axillary Bud Swelling of Hevea brasiliensis to Regenerate Plants through Somatic Embryogenesis and Analysis of Genetic Stability

Wang

Udayabhanu

et al. 2023

Plants

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To overcome rubber tree (RT) tissue culture explant source limitations, the current study aimed to establish a new Hevea brasiliensis somatic embryogenesis (SE) system, laying the technical foundation for the establishment of an axillary-bud-based seedling regeneration system. In this study, in vitro plantlets of Hevea brasiliensis Chinese Academy of Tropical Agricultural Sciences 917 (CATAS 917) were used as the experimental materials. Firstly, the optimum conditions for axillary bud swelling were studied; then, the effects of phenology, the swelling time of axillary buds (ABs), and medium of embryogenic callus induction were studied. Plantlets were obtained through somatic embryogenesis. Flow cytometry, inter-simple sequence repeat (ISSR molecular marker) and chromosome karyotype analysis were used to study the genetic stability of regenerated plants along with budding seedlings (BSs) and secondary somatic embryo seedlings (SSESs) as the control. The results show that the rubber tree’s phenology period was mature, and the axillary bud induction rate was the highest in the 2 mg/L 6-benzyladenine (6-BA) medium (up to 85.83%). Later, 3-day-old swelling axillary buds were used as explants for callogenesis and somatic embryogenesis. The callus induction rate was optimum in MH (Medium in Hevea) + 1.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) + 1.5 mg/L 1-naphthalene acetic acid (NAA) + 1.5 mg/L Kinetin (KT) + 70 g/L sucrose (56.55%). The regenerated plants were obtained after the 175-day culture of explants through callus induction, embryogenic callus induction, somatic embryo development, and plant regeneration. Compared with the secondary somatic embryo seedling control, axillary bud regeneration plants (ABRPs) were normal diploid plants at the cellular and molecular level, with a variation rate of 7.74%.

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Section: Introductionmentioning

confidence: 99%

Induction of Axillary Bud Swelling of Hevea brasiliensis to Regenerate Plants through Somatic Embryogenesis and Analysis of Genetic Stability

Wang

Udayabhanu

et al. 2023

Plants

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Fitting levels of 6-benzylademine matching seasonal explants effectively stimulate adventitious shoot induction in Cyclocarya paliurus (Batal.) Iljinskaja

Cheng,

Cui,

Shang

et al. 2023

Plant Cell Tiss Organ Cult

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Phytohormone additions match explants stimulating adventitious shoot induction in Cyclocarya paliurus (Batal.) Iljinskaja

Yuan

Cui

Shang

et al. 2023

Preprint

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As an endemic medicinal woody plant in China, Cyclocarya paliurus (Batal.) Iljinskajais is being extensively planted for leaf-use. Operational system of tissue culture mainly depends on explants from short growth season. To extend sampling season, an efficient tissue culture system for nodal stem segments across annual growth seasons has been established in C. paliurus. Explants collected at three stages (March-May, stage I; June-August stage Ⅱ; September-November, stage Ⅲ) were cultured in MS, supplemented with gradient concentrations of 6-benzylademine (BA) (2.0–12.0 mg·L− 1) for shoot induction. The relationships of phytohomones between endogenesis in explants and exogenous addition in medium were also analyzed by using artificial neural networks (ANN) for the optimal concentration of 6-BA addition. The optimal adventitious shoot induction happened on MS supplemented 6-BA with 4.0 mg·L− 1 for stage Ⅰ, 6.0 mg·L− 1 for stage Ⅱ, and 12.0 mg·L− 1 for stage Ⅲ; their induction ratios reached 92.9%, 96.67% and 82.33%, respectively. Regardless of growth stage, adventitious buds occurred on the 20th day when the content of ABA at the bottom versus IAA on the peak. Moreover, high ABA content in explants of C. paliurus (128.49-325.79 ng/g FW) is considered as the main inhibitor for induction. A model constructed by ANN based on a set of validation data, displayed good agreement with observed results. Also, the combination addition (0.5 mg·L− 1 6-BA + 1.5 mg·L− 1 IAB + 0.03 mg·L− 1 TIBA) to MS for shoot multiplication attained 3.20 of multiplication coefficient. Although rooting induction achieved to 66.67% when supplemented 11.0 mg·L− 1 IBA to WPM, no plantlets survived after acclimatization. We speculate that the high 6-BA content for adventitious buds induction could be responsible for lower rooting rate and transplanting failure. Therefore, more efforts should be done to build a feasible tissue culture system in C. paliurus.

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Effect of forcing solutions used to break the seasonal influence on in vitro axillary bud sprouting of two Leucospermum (R. Br.) cultivars

Cited by 3 publications

References 16 publications

Induction of Axillary Bud Swelling of Hevea brasiliensis to Regenerate Plants through Somatic Embryogenesis and Analysis of Genetic Stability

Induction of Axillary Bud Swelling of Hevea brasiliensis to Regenerate Plants through Somatic Embryogenesis and Analysis of Genetic Stability

Fitting levels of 6-benzylademine matching seasonal explants effectively stimulate adventitious shoot induction in Cyclocarya paliurus (Batal.) Iljinskaja

Phytohormone additions match explants stimulating adventitious shoot induction in Cyclocarya paliurus (Batal.) Iljinskaja

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