Structural and functional evaluation of oxygenating keratin/silk fibroin scaffold and initial assessment of their potential for urethral tissue engineering

Lv, Xiangguo; Li, Zhe; Chen, ShiYan; Xie, Minkai; Huang, Jianwen; Peng, Xufeng; Yang, Rui; Wang, Huaping; Xu, Yuhong; Cao, Feng

doi:10.1016/j.biomaterials.2016.01.032

Cited by 102 publications

(67 citation statements)

References 44 publications

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“…Lv et al reported that films containing CPO (20 w/w%) presented an excellent cell response in vitro for proliferation. 64 The oxygen solubility will be proportional to the partial pressure (Henry's law); therefore, oxygen-generating biomaterials store an adequate amount of oxygen due to the van der Waals interaction between the oxygen and the surface of the materials. Thus, the release of oxygen will not only occur in the implanted areas, but the complete oxygenation in the microenvironments will positively affect and support the growth of cells in the neighboring tissues that may be found in a hypoxia environment.…”

Section: Resultsmentioning

confidence: 99%

<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

Morais¹,

Wang²,

Vieira³

et al. 2020

IJN

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Background: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications. Methods: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis. Results: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ =-16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M. Conclusion: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.

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Section: Resultsmentioning

confidence: 99%

<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

Morais¹,

Wang²,

Vieira³

et al. 2020

IJN

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“…Both accellular and cell seeded SF showed good efficacy in urethral reconstruction in animal model [26,29,30]. Recently, Lv et al [31] reported a novel oxygen-generating material composed of SF, keratin, calcium peroxide and gelatin. This study showed 3D porous structure, high mechanical property and steady release of oxygen, which improved the urethral tissue regeneration in dogs.…”

Section: Protein Derived Scaffolds and Cellulosementioning

confidence: 99%

Biomatrices in Urethral Reconstruction

Long¹,

Xiao²

2016

J Biotechnol Biomater

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Urethral reconstruction is still a big challenge in urology. Traditionally, the penile skin or buccal mucosa was used as graft for replacement. However, the morbidity of the donor site was reported previously. Furthermore, there is a lack of an adequate autologous donor graft in many cases. The tissue engineered urethra may provide an alternative to the reconstruction. Herein, we reviewed the biomatrices for urethral substitution either in animal model or in clinical cases.

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“…A variety of diseases, such as congenital diseases, malignancies and trauma, can lead to anterior urethral defects or strictures, which severely in uence the physical condition and quality of life of patients [1].…”

Section: Introductionmentioning

confidence: 99%

Hypoxia-Preconditioned Adipose-Derived Stem Cells Combined with Scaffold Promote Urethral Reconstruction by Up-Regulation of Angiogenesis and Glycolysis

Xiang¹,

Xie²,

Xu³

et al. 2020

Preprint

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Rationale: Tissue engineering is a promising alternative for urethral reconstruction, and adipose-derived stem cells (ADSCs) are widely used as seeding cells. Hypoxia preconditioning can significantly enhance the therapeutic effects of ADSCs. The low oxygen tension of postoperative wound healing is inevitable and may facilitate the nutritional function of ADSCs. This study aimed to investigate if hypoxia preconditioned ADSCs, compared to normxia preconditioned ADSCs, combined with scaffold could better promote urethral reconstruction and exploring the underlying mechanism.Methods: In vitro, paracrine cytokines and secretomes that were secreted by hypoxia- or normoxia-preconditioned ADSCs were added to cultures of human umbilical vein endothelial cells (HUVECs) to measure their functions. In vivo, hypoxia- or normoxia-preconditioned ADSCs were seeded on a porous nanofibrous scaffold for urethral repair on a defect model in rabbits.Results: The in vitro results showed that hypoxia could enhance the secretion of VEGFA by ADSCs, and hypoxia-preconditioned ADSCs could enhance the viability, proliferation, migration, angiogenesis and glycolysis of HUVECs (p < 0.05). After silencing VEGFA, angiogenesis and glycolysis were significantly inhibited (p < 0.05). The in vivo results showed that compared to normoxia-preconditioned ADSCs, hypoxia-preconditioned ADSCs combined with scaffolds led to a larger urethral lumen diameter, preserved urethral morphology and enhanced angiogenesis (p < 0.05). Conclusions: Hypoxia preconditioning of ADSCs combined with scaffold could better promote urethral reconstruction by upregulating angiogenesis and glycolysis. Hypoxia-preconditioned ADSCs combined with novel scaffold may provide a promising alternative treatment for urethral reconstruction.

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Structural and functional evaluation of oxygenating keratin/silk fibroin scaffold and initial assessment of their potential for urethral tissue engineering

Cited by 102 publications

References 44 publications

<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

Biomatrices in Urethral Reconstruction

Hypoxia-Preconditioned Adipose-Derived Stem Cells Combined with Scaffold Promote Urethral Reconstruction by Up-Regulation of Angiogenesis and Glycolysis

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