&lt;p&gt;Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications&lt;/p&gt;

Morais, Alan Is; Wang, Xichi; Vieira, Ewerton Gomes; Viana, Bartolomeu C.; Filho, Edson Cavalcanti da Silva; Osajima, Josy A.; Afewerki, Samson; Corat, Marcus Alexandre Finzi; Silva, Heurison S.; Marciano, Fernanda Roberta; Ruiz–Esparza, Guillermo U.; Stocco, Thiago Domingues; Paula, Mirian Mm de; Lobo, Anderson Oliveira

doi:10.2147/ijn.s237334

Cited by 16 publications

(3 citation statements)

References 72 publications

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“…This is a further indication the effect these stabilizing groups can have on hydrophobic polymer, bringing a change in the overall output in the form of the type of species one can produce—particles or fibers. This result could be associated with selected applied electrical field and solution parameters during the ES process as previously described from our group elsewhere [ 13 , 14 ]. Particle size distribution analysis indicated that all samples have average sizes between 1 and 10 μm.…”

Section: Resultsmentioning

confidence: 90%

Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels

Hassan

Çeçen

Peña-Garcia

et al. 2021

JFB

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Different strategies have been employed to provide adequate nutrients for engineered living tissues. These have mainly revolved around providing oxygen to alleviate the effects of chronic hypoxia or anoxia that result in necrosis or weak neovascularization, leading to failure of artificial tissue implants and hence poor clinical outcome. While different biomaterials have been used as oxygen generators for in vitro as well as in vivo applications, certain problems have hampered their wide application. Among these are the generation and the rate at which oxygen is produced together with the production of the reaction intermediates in the form of reactive oxygen species (ROS). Both these factors can be detrimental for cell survival and can severely affect the outcome of such studies. Here we present calcium peroxide (CPO) encapsulated in polycaprolactone as oxygen releasing microparticles (OMPs). While CPO releases oxygen upon hydrolysis, PCL encapsulation ensures that hydrolysis takes place slowly, thereby sustaining prolonged release of oxygen without the stress the bulk release can endow on the encapsulated cells. We used gelatin methacryloyl (GelMA) hydrogels containing these OMPs to stimulate survival and proliferation of encapsulated skeletal myoblasts and optimized the OMP concentration for sustained oxygen delivery over more than a week. The oxygen releasing and delivery platform described in this study opens up opportunities for cell-based therapeutic approaches to treat diseases resulting from ischemic conditions and enhance survival of implants under severe hypoxic conditions for successful clinical translation.

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Section: Resultsmentioning

confidence: 90%

Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels

Hassan

Çeçen

Peña-Garcia

et al. 2021

JFB

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“…Adding CaO 2 at 8-12 mg/mL concentration within gelatin/hyaluronan matrix enabled the fabrication of sprayable hydrogel dressings with prolonged oxygen-releasing capacity (2 weeks), antibacterial properties, and an important ability to diminish the metabolic stress and reduce cell death in hypoxic fibroblasts [135]. Given their extended-release oxygen profile, CaO 2 -loaded polyester microspheres (either solitary or encapsulated within a self-polymerized peptide hydrogel) exhibited long-term beneficial effects on normal cells [136,137]. An oxygen-generating implantable material consisting of alginate beads encapsulating CaO 2 and catalase has been developed by Huang and colleagues [138] as an active carrier for doxorubicin, with the aim to increase the drug sensitivity in cancer cells undergoing hypoxia-induced chemo-resistance.…”

Section: Discussionmentioning

confidence: 99%

Infection-Free and Enhanced Wound Healing Potential of Alginate Gels Incorporating Silver and Tannylated Calcium Peroxide Nanoparticles

Bîrcă,

Gherasim,

Niculescu

et al. 2024

IJMS

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The treatment of chronic wounds involves precise requirements and complex challenges, as the healing process cannot go beyond the inflammatory phase, therefore increasing the healing time and implying a higher risk of opportunistic infection. Following a better understanding of the healing process, oxygen supply has been validated as a therapeutic approach to improve and speed up wound healing. Moreover, the local implications of antimicrobial agents (such as silver-based nano-compounds) significantly support the normal healing process, by combating bacterial contamination and colonization. In this study, silver (S) and tannylated calcium peroxide (CaO2@TA) nanoparticles were obtained by adapted microfluidic and precipitation synthesis methods, respectively. After complementary physicochemical evaluation, both types of nanoparticles were loaded in (Alg) alginate-based gels that were further evaluated as possible dressings for wound healing. The obtained composites showed a porous structure and uniform distribution of nanoparticles through the polymeric matrix (evidenced by spectrophotometric analysis and electron microscopy studies), together with a good swelling capacity. The as-proposed gel dressings exhibited a constant and suitable concentration of released oxygen, as shown for up to eight hours (UV–Vis investigation). The biofilm modulation data indicated a synergistic antimicrobial effect between silver and tannylated calcium peroxide nanoparticles, with a prominent inhibitory action against the Gram-positive bacterial biofilm after 48 h. Beneficial effects in the human keratinocytes cultured in contact with the obtained materials were demonstrated by the performed tests, such as MTT, LDH, and NO.

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“…For instance, CaO 2 particles can be coated with biodegradable polyesters such as poly(ϵ-caprolactone) (PCL) or poly (lactic- co -glycolic acid) (PLGA) to control and obtain a sustained release of O 2 . Additionally, alternate strategies to prolong O 2 release can be explored, such as other oxides (e.g., zinc oxide, manganese dioxide) and peroxides (e.g., magnesium peroxide, H 2 O 2 ), perfluorocarbons, and percarbonates ( 29 , 41 , 56 – 58 ).…”

Section: Discussionmentioning

confidence: 99%

Biomaterial-assisted local oxygenation safeguards the prostimulatory phenotype and functions of human dendritic cells in hypoxia

Bhatt,

Nukovic,

Colombani

et al. 2023

Front. Immunol.

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Dendritic cells (DCs), professional antigen-presenting cells, function as sentinels of the immune system. DCs initiate and fine-tune adaptive immune responses by presenting antigenic peptides to B and T lymphocytes to mount an effective immune response against cancer and pathogens. However, hypoxia, a condition characterized by low oxygen (O2) tension in different tissues, significantly impacts DC functions, including antigen uptake, activation and maturation, migration, as well as T-cell priming and proliferation. In this study, we employed O2-releasing biomaterials (O2-cryogels) to study the effect of localized O2 supply on human DC phenotype and functions. Our results indicate that O2-cryogels effectively mitigate DC exposure to hypoxia under hypoxic conditions. Additionally, O2-cryogels counteract hypoxia-induced inhibition of antigen uptake and migratory activity in DCs through O2 release and hyaluronic acid (HA) mediated mechanisms. Furthermore, O2-cryogels preserve and restore DC maturation and co-stimulation markers, including HLA-DR, CD86, and CD40, along with the secretion of proinflammatory cytokines in hypoxic conditions. Finally, our findings demonstrate that the supplemental O2 released from the cryogels preserves DC-mediated T-cell priming, ultimately leading to the activation and proliferation of allogeneic CD3+ T cells. This work emphasizes the potential of local oxygenation as a powerful immunomodulatory agent to improve DC activation and functions in hypoxia, offering new approaches for cancer and infectious disease treatments.

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<p>Electrospraying Oxygen-Generating Microparticles for Tissue Engineering Applications</p>

Cited by 16 publications

References 72 publications

Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels

Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels

Infection-Free and Enhanced Wound Healing Potential of Alginate Gels Incorporating Silver and Tannylated Calcium Peroxide Nanoparticles

Biomaterial-assisted local oxygenation safeguards the prostimulatory phenotype and functions of human dendritic cells in hypoxia

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