Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis

Rodrigues, Raphael Silva; Sopelete, Mônica Camargo; Silva, Deise Aparecida de Oliveira; Cunha-Júnior, Jair P.; Taketomi, Ernesto Akio; Costa-Cruz, Júlia Maria

doi:10.1590/s0074-02762004000100016

Cited by 24 publications

(19 citation statements)

References 24 publications

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“…Thus, studies have been conducted using heterologous antigen of filariform larvae from rodents as Strongyloides ratti in the development of serological methods (Grove and Blair 1981;Costa-Cruz et al 1997;Rodrigues et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown that high levels of IgG, IgE, and IgA antibodies play an important role in the immune response to helminths (Genta and Lillibridge 1989;Rodrigues et al 2004). High levels of specific IgE are usually demonstrated in immunocompetent patients with strongyloidiasis, whereas lower levels are found in the disseminated disease and immunosuppression conditions (Atkins et al 1997;Vercelli et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen

et al. 2007

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The aim of this study was to evaluate total IgG, IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by enzyme-linked immunosorbent assay (ELISA) using Strongyloides ratti saline extract as heterologous antigen for a possible clinical utility of the assay. A total of 40 serum samples of patients who were shedding Strongyloides stercoralis larvae in feces (group I), 30 sera from patients with other intestinal parasites (group II), and 30 sera from subjects with negative results in three parasitological assays (group III) were analyzed to detect total IgG, IgG1, IgG4, and IgE to Strongyloides spp. by ELISA and expressed in ELISA index. Levels of total IgG anti-Strongyloides spp. were significantly higher in patients of group I than in groups II (p=0.0005) and III (p<0.0001). Levels of specific IgG1, IgG4, and IgE of group I were also significantly higher than in groups II and III, respectively. There was a significant positive correlation between specific IgE and IgG4 (r=0.6524; p=0.0084) and IgG1 and IgG4 (r=0.5398; p=0.0171). It can be concluded that the detection of specific IgE, IgG1, and IgG4 subclasses rather than total IgG antibodies to Strongyloides spp. using the S. ratti antigen showed to be an additional tool for improving the serodiagnosis of human strongyloidiasis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen

et al. 2007

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“…Antigenic preparations resulting from a saline extract from S. stercoralis larvae or from heterologous parasites, such as S. ratti and S. venezuelensis have been used for antibodies detection in an ELISA format for strongyloidiasis diagnosis (CostaCruz et al 2003;Rodrigues et al 2004;Sato et al 1995a;Silva et al 2003). Despite the great advances in using heterologous antigen for the diagnosis of S. stercoralis in ELISA and immunoblot, more research is necessary to compare the immunodominant antigenic components of those species with S. stercoralis.…”

Section: Introductionmentioning

confidence: 99%

Identification of a 26-kDa protein fraction as an important antigen for application in the immunodiagnosis of strongyloidiasis

et al. 2007

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Strongyloidiasis caused by the intestinal nematode Strongyloides stercoralis typically occurs in the asymptomatic form. The definitive diagnosis is usually done by detection of larvae on fecal samples. However, as the parasite load is often low in most cases, microscopy is not usually sensitive and specific, and diagnosis becomes extremely difficult. Thus, development of reliable serological methods is imperative. In the present study, a diversity of epitopes from S. stercoralis larva were characterized by analysis of reactivity with serum samples obtained from individuals with and without the infection by using Western blot technique. A total of 91 serum samples belonging to 5 groups were analyzed. Different reactivity profiles were observed, representing recognition of proteins with molecular mass varied from 6 to 129 kDa. A protein band of approximately 26 kDa presented a high frequency of reactivity with serum samples from the strongyloidiasis patients group (18/23). Reactivity with this protein band was also observed in only 7 of 64 non-infected individuals or individuals infected with other helminthes. Reactivity with 2 other bands, 1 of approximately 33 kDa and a duplet of approximately 21 kDa, were also found in high frequency (17/23 and 9/23, respectively). However, reactivity with these bands was also observed in all the other serum groups studied. The results indicate that the 26-kDa band maybe be an important tool for the development of diagnostic techniques for strongyloidiasis.

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“…The majority of cases involve chronic lowlevel infection with a minimal and irregular larval output [6,8,9]. Immunologic detection of specific antibodies against Strongyloides is useful for diagnosis because it complements parasitological analysis [10][11][12][13]. Serum is generally used for the detection of specific antibodies.…”

Section: Introductionmentioning

confidence: 99%

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

et al. 2010

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Strongyloides stercoralis causes chronic asymptomatic infections in immunocompetent human hosts and systemic invasion in immunocompromised patients, developing into a fatal hyperinfection syndrome. IgA and IgG detection in saliva and serum paired samples were tested using total saline extract from Strongyloides venezuelensis (SE(*)) and its detergent phase (D) extracted with Triton X-114. Saliva and serum paired samples were obtained from: 25 patients with confirmed strongyloidiasis; 25 patients with other parasitoses and 20 from apparently healthy individuals. Sensitivity, specificity, diagnostic efficiency, positive and negative predictive values and likelihood ratio were calculated at the optimum point of reaction. Using D phase sensitivity and specificity to detect IgA in saliva were 76.0% and 88.9% and in serum 80.0% and 86.7%, respectively. To detect IgG, D phase showed sensitivity and specificity of 88.0% and 88.9% in saliva and 88.0% and 84.4% in serum, respectively. D phase proved to be specific and efficient and could be utilized as an alternative antigen for IgA and IgG detection in saliva and serum samples for strongyloidiasis diagnosis.

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Strongyloides ratti antigenic components recognized by IgE antibodies in immunoblotting as an additional tool for improving the immunodiagnosis in human strongyloidiasis

Cited by 24 publications

References 24 publications

IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen

IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen

Identification of a 26-kDa protein fraction as an important antigen for application in the immunodiagnosis of strongyloidiasis

Detergent fraction of heterologous antigen to detect IgA and IgG in strongyloidiasis using saliva and serum paired samples

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