Streptococcus suis serotype 9 strain GZ0565 contains a type VII secretion system putative substrate EsxA that contributes to bacterial virulence and a vanZ- like gene that confers resistance to teicoplanin and dalbavancin in Streptococcus agalactiae

Lai, Liying; Dai, Jiao; Tang, Huanyu; Zhang, Shouming; Wu, Chunyan; Qiu, Wancen; Chen, Lu; Yao, Huochun; Fan, Hongjie; Wu, Zongfu

doi:10.1016/j.vetmic.2017.04.030

Cited by 38 publications

(45 citation statements)

References 35 publications

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“…Surprisingly, when expressed in S. aureus, enterococcal genomic vanZ g , which is not associated with any glycopeptide resistance gene cluster, was more efficient in conferring resistance to TEI than vanZ Tei ( Table 1). In addition to E. faecium and S. pneumoniae, the involvement of genomic vanZ genes in the resistance to lipoglycopeptide antibiotics was reported for orthologs from Streptococcus suis and Clostridium difficile (Lai et al, 2017;Woods et al, 2018; Supplementary Figure S2). In addition to resistance, VanZ proteins might play a more general role in stress response and virulence, as it was observed that the expression of the streptococcal vanZ gene was induced by the epithelial antimicrobial peptide LL37 (Lai et al, 2017) and ribosome-targeting antibiotics (Ng et al, 2003) or was essential for lung infection (Hava and Camilli, 2002).…”

Section: Discussionmentioning

confidence: 95%

VanZ Reduces the Binding of Lipoglycopeptide Antibiotics to Staphylococcus aureus and Streptococcus pneumoniae Cells

et al. 2020

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vanZ, a member of the VanA glycopeptide resistance gene cluster, confers resistance to lipoglycopeptide antibiotics independent of cell wall precursor modification by the vanHAX genes. Orthologs of vanZ are present in the genomes of many clinically relevant bacteria, including Enterococcus faecium and Streptococcus pneumoniae; however, vanZ genes are absent in Staphylococcus aureus. Here, we show that the expression of enterococcal vanZ paralogs in S. aureus increases the minimal inhibitory concentrations of lipoglycopeptide antibiotics teicoplanin, dalbavancin, oritavancin and new teicoplanin pseudoaglycone derivatives. The reduction in the binding of fluorescently labeled teicoplanin to the cells suggests the mechanism of VanZ-mediated resistance. In addition, using a genomic vanZ gene knockout mutant of S. pneumoniae, we have shown that the ability of VanZ proteins to compromise the activity of lipoglycopeptide antibiotics by reducing their binding is a more general feature of VanZ-superfamily proteins.

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Section: Discussionmentioning

confidence: 95%

VanZ Reduces the Binding of Lipoglycopeptide Antibiotics to Staphylococcus aureus and Streptococcus pneumoniae Cells

et al. 2020

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“…This latter carries four different AMR genes: aph(3')-IIIa-vanZ-sat4-ant(6)-Ia (Figure 8) conferring resistance to kanamycin, teicoplanin (low-resistance), streptothricin, and streptomycin, respectively. The encoded VanZ protein displays only 38% of identity with the one previously described in S. suis GZ0565 [25]. However, interestingly, it shows 100% of identity with the one encoded by an IME integrated inside a Tn1549-related ICE-itself integrated with a Tn5252-related ICE-in Streptococcus lutetiensis (ICESluvan) [17,26].…”

Section: Imes Carrying Amr Genes In S Suismentioning

confidence: 87%

Chromosomal Conjugative and Mobilizable Elements in Streptococcus suis: Major Actors in the Spreading of Antimicrobial Resistance and Bacteriocin Synthesis Genes

et al. 2019

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Streptococcus suis is a zoonotic pathogen suspected to be a reservoir of antimicrobial resistance (AMR) genes. The genomes of 214 strains of 27 serotypes were screened for AMR genes and chromosomal Mobile Genetic Elements (MGEs), in particular Integrative Conjugative Elements (ICEs) and Integrative Mobilizable Elements (IMEs). The functionality of two ICEs that host IMEs carrying AMR genes was investigated by excision tests and conjugation experiments. In silico search revealed 416 ICE-related and 457 IME-related elements. These MGEs exhibit an impressive diversity and plasticity with tandem accretions, integration of ICEs or IMEs inside ICEs and recombination between the elements. All of the detected 393 AMR genes are carried by MGEs. As previously described, ICEs are major vehicles of AMR genes in S. suis. Tn5252-related ICEs also appear to carry bacteriocin clusters. Furthermore, whereas the association of IME-AMR genes has never been described in S. suis, we found that most AMR genes are actually carried by IMEs. The autonomous transfer of an ICE to another bacterial species (Streptococcus thermophilus)—leading to the cis-mobilization of an IME carrying tet(O)—was obtained. These results show that besides ICEs, IMEs likely play a major role in the dissemination of AMR genes in S. suis.

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“…The detailed protocol for expressing and purifying tNT was described in our previous study [27]. GroEL was expressed and purified in our previous study [28]. …”

Section: Methodsmentioning

confidence: 99%

“…The cells were then lysed, and cellular proteins were separated by 15% SDS-PAGE; the resolved proteins were transferred onto a PVDF membrane (GE Healthcare, USA). The detailed procedure for Western blotting was described in our previous study [28]. The antibody against the cleaved caspase-3 or antibody against β-actin (1:1000 dilution, Cell Signaling Technology, USA) were used as the primary antibody.…”

Section: Methodsmentioning

confidence: 99%

Streptococcus suis synthesizes deoxyadenosine and adenosine by 5’-nucleotidase to dampen host immune responses

et al. 2018

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Streptococcus suis is a major porcine bacterial pathogen and emerging zoonotic agent. S. suis 5ʹ-nucleotidase is able to convert adenosine monophosphate to adenosine, resulting in inhibiting neutrophil functions in vitro and it is an important virulence factor. Here, we show that S. suis 5ʹ-nucleotidase not only enables producing 2ʹ-deoxyadenosine from 2ʹ-deoxyadenosine monophosphate by the enzymatic assay and reversed-phase high performance liquid chromatography (RP-HPLC) analysis in vitro, but also synthesizes both 2ʹ-deoxyadenosine and adenosine in mouse blood in vivo by RP-HPLC and liquid chromatography with tandem mass spectrometry analyses. Cellular cytotoxicity assay and Western blot analysis indicated that the production of 2ʹ-deoxyadenosine by 5ʹ-nucleotidase triggered the death of mouse macrophages RAW 264.7 in a caspase-3-dependent way. The in vivo infection experiment showed that 2ʹ-deoxyadenosine synthesized by 5ʹ-nucleotidase caused monocytopenia in mouse blood. The in vivo transcriptome analysis in mouse blood showed the inhibitory effect of 5ʹ-nucleotidase on neutrophil functions and immune responses probably mediated through the generation of adenosine. Taken together, these findings indicate that S. suis synthesizes 2ʹ-deoxyadenosine and adenosine by 5ʹ-nucleotidase to dampen host immune responses, which represents a new mechanism of S. suis pathogenesis.

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Streptococcus suis serotype 9 strain GZ0565 contains a type VII secretion system putative substrate EsxA that contributes to bacterial virulence and a vanZ- like gene that confers resistance to teicoplanin and dalbavancin in Streptococcus agalactiae

Cited by 38 publications

References 35 publications

VanZ Reduces the Binding of Lipoglycopeptide Antibiotics to Staphylococcus aureus and Streptococcus pneumoniae Cells

VanZ Reduces the Binding of Lipoglycopeptide Antibiotics to Staphylococcus aureus and Streptococcus pneumoniae Cells

Chromosomal Conjugative and Mobilizable Elements in Streptococcus suis: Major Actors in the Spreading of Antimicrobial Resistance and Bacteriocin Synthesis Genes

Streptococcus suis synthesizes deoxyadenosine and adenosine by 5’-nucleotidase to dampen host immune responses

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