Chromosomal Conjugative and Mobilizable Elements in Streptococcus suis: Major Actors in the Spreading of Antimicrobial Resistance and Bacteriocin Synthesis Genes

Libante, Virginie; Nombre, Yves; Coluzzi, Charles; Staub, Johan; Guédon, Gérard; Gottschalk, Marcelo; Teatero, Sarah; Fittipaldi, Nahuel; Leblond‐Bourget, Nathalie; Payot, Sophie

doi:10.3390/pathogens9010022

Cited by 30 publications

(79 citation statements)

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“…The vast majority of these integrated mobile elements carry adaptive genes that may confer on their host a significant selective advantage or may change their lifestyle (e.g., antibiotic, heavy metal or phage resistance, sucrose catabolism, bacteriocin synthesis, pathogenicity or symbiosis) [2,3,10,[18][19][20][21]. It is therefore important to study the prevalence of these elements and to identify the adaptive function they tend to disseminate among bacterial populations.…”

Section: Introductionmentioning

confidence: 99%

Abundance, Diversity and Role of ICEs and IMEs in the Adaptation of Streptococcus salivarius to the Environment

Lao

Guédon

Lacroix

et al. 2020

Genes

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Streptococcus salivarius is a significant contributor to the human oral, pharyngeal and gut microbiomes that contribute to the maintenance of health. The high genomic diversity observed in this species is mainly caused by horizontal gene transfer. This work aimed to evaluate the contribution of integrative and conjugative elements (ICEs) and integrative and mobilizable elements (IMEs) in S. salivarius genome diversity. For this purpose, we performed an in-depth analysis of 75 genomes of S. salivarius and searched for signature genes of conjugative and mobilizable elements. This analysis led to the retrieval of 69 ICEs, 165 IMEs and many decayed elements showing their high prevalence in S. salivarius genomes. The identification of almost all ICE and IME boundaries allowed the identification of the genes in which these elements are inserted. Furthermore, the exhaustive analysis of the adaptation genes carried by these elements showed that they encode numerous functions such as resistance to stress, to antibiotics or to toxic compounds, and numerous enzymes involved in diverse cellular metabolic pathways. These data support the idea that not only ICEs but also IMEs and decayed elements play an important role in S. salivarius adaptation to the environment.

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Section: Introductionmentioning

confidence: 99%

Abundance, Diversity and Role of ICEs and IMEs in the Adaptation of Streptococcus salivarius to the Environment

Lao

Guédon

Lacroix

et al. 2020

Genes

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“…Antimicrobial resistance is indeed one of the major global health challenges of this century, and S. suis is suspected to be a reservoir of antimicrobial resistance genes [17]. Libante et al [18] screened the genomes of 214 strains of 27 serotypes for antimicrobial resistance genes and chromosomal Mobile Genetic Elements (MGEs), in particular Integrative Conjugative Elements (ICEs) and Integrative Mobilizable Elements (IMEs). This large-scale genome analysis revealed the high diversity of putative MGEs transferring by conjugation (ICEs and IMEs) in S. suis.…”

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confidence: 99%

“…These results suggest that besides ICEs, IMEs likely play a major role in the dissemination of antimicrobial resistance genes in S. suis. The work of Libante et al [18] highlights the importance of reducing the prophylactic use of antimicrobials in livestock productions and the development of alternative control measures. In this regard, Wongsawan et al [19] explored the bactericidal effect of clove oil against S. suis in vitro.…”

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confidence: 99%

“…The analysis of the phylogenetic structure of the ST7 population, including epidemic and sporadic ST7 strains, revealed high diversity. Compared to the genome of the epidemic strain, the major differences in the genome of sporadic ST7 strains were the absence of the 89 kb SsPI-1 (specific to the epidemic strain) and the insertion of MGEs or ICE elements, suggesting a role in horizontal transfer of antibiotic resistance genes [18]. The emergence of these ST7 strains, and importantly within serotype 14 (reported by Wang et al for the first time [47]), highlights the need to have a better understanding of the evolution of zoonotic ST7 strains and their ability to cause life-threatening infections in humans.…”

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Streptococcus suis Research: Progress and Challenges

Segura

2020

Pathogens

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Streptococcus suis is considered among the top bacterial pathogens leading to important economic losses to the swine industry, with the incidence of disease increasing as the prophylactic use of antimicrobial is being vanished worldwide. S. suis is also a zoonotic agent afflicting people in close contact with infected pigs or pork meat. Besides, in some Asian countries, it is considered a major public health concern for the general population as well. Antimicrobial resistance is one of the most important global health challenges, and in the absence of preventive measures (such as effective vaccines), S. suis remains a risk for increased antimicrobial resistance and transmission of resistance genes to other bacteria beyond the host animal species. The studies in this Special Issue have evidenced the importance of swine population demographics and management on disease control, progress in molecular tools to better understand the epidemiology of S. suis infections in swine and humans, and the mechanisms involved in different aspects of the immuno-pathogenesis of the disease. The importance of reducing the prophylactic use of antimicrobials in livestock productions and the development of alternative control measures, including vaccination, are herein discussed.

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“…Besides genes conferring antibiotic resistance, ICEs may harbour additional cargo genes that provide an adaptive advantage over other elements. Some of these examples include the presence of the siderophore yersiniabactin encoded within the ICEKp in hypervirulent clonal-group CG23 Klebsiella pneumoniae [7]; the Tn5252-related ICEs carrying bacteriocin clusters in Streptococcus suis [8]; the type I-C CRISPR-Cas systems identified within pKLC102-like ICEs in P. aeruginosa [9] and the type III restriction-modification systems from SXT/R391-related ICEs in Shewanella spp. [10].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive genome data analysis establishes a triple whammy of carbapenemases, ICEs and multiple clinically-relevant bacteria

Botelho

Mourão

Roberts

et al. 2019

Preprint

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28Carbapenemases inactivate most β-lactam antibiotics, including carbapenems and have 29 been frequently reported among Enterobacteriaceae, Acinetobacter spp. and 30 Pseudomonas spp. Traditionally, the horizontal gene transfer of carbapenemase-31 encoding genes (CEGs) has been linked to plasmids. However, given that integrative and 32 conjugative elements (ICEs) are possibly the most abundant conjugative elements 33 among prokaryotes, we conducted an in silico analysis to ascertain the likely role of ICEs 34 in the spread of CEGs among all bacterial genomes. We detected 7457 CEGs, of which 35 224 were located within putative ICEs among several clinically-relevant bacterial species 36 (including Klebsiella pneumoniae, Escherichia coli, Citrobacter freundii, Enterobacter 37 hormaechei and Pseudomonas aeruginosa). Most CEGs detected within ICEs belong to 38 the KPC, IMP and NDM families and different mechanisms were likely responsible for 39 acquisition of these genes. The majority of CEG-bearing ICEs belong to the MPFT, MPFF, 40 MPFG and MPFI classes and often encode additional adaptive traits such as resistance to 41 other antibiotics (e.g. aminoglycosides and fluoroquinolones), metal and biocide 42 tolerance and bacteriocin production. This study provides a snapshot of the different 43 CEGs associated with ICEs among all bacterial genomes and sheds light on the 44 underappreciated contribution of ICEs to the spread of carbapenem resistance globally.

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Chromosomal Conjugative and Mobilizable Elements in Streptococcus suis: Major Actors in the Spreading of Antimicrobial Resistance and Bacteriocin Synthesis Genes

Cited by 30 publications

References 54 publications

Abundance, Diversity and Role of ICEs and IMEs in the Adaptation of Streptococcus salivarius to the Environment

Abundance, Diversity and Role of ICEs and IMEs in the Adaptation of Streptococcus salivarius to the Environment

Streptococcus suis Research: Progress and Challenges

Comprehensive genome data analysis establishes a triple whammy of carbapenemases, ICEs and multiple clinically-relevant bacteria

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