Strategy for setting up single-capillary isotachophoresis—zone electrophoresis

Reinhoud, N.J.; Tjaden, U.R.; Greef, J. van der

doi:10.1016/0021-9673(93)83188-x

Cited by 54 publications

(42 citation statements)

References 21 publications

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“…These solutes can be stacked in an uncoated capillary, using the TE buffer as BGE buffer in combination with a negative backpressure, as described by Reinhoud et aZ. [15]. Unfortunately, this strategy has some disadvantages.…”

Section: Stacking Of Anionic Compoundsmentioning

confidence: 98%

Development and validation of transient isotachophoretic capillary zone electrophoresis for determination of peptides

et al. 1998

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Although capillary zone electrophoresis (CZE) is known for its high resolution power and low mass detection limits, the concentration detection limits are rather poor when ultraviolet absorbance detection is used. To overcome this limitation, several on-column transient isotachophoresis (tITP) protocols have been developed and validated for the determination of both cationic and anionic model peptides, separately. Using this preconcentration method, up to 72% of the capillary can be filled with sample solution, without any loss in resolution. Thus, without any modification of the hardware set-up, the sensitivity is increased about two orders of magnitude. For the model cationic peptides (gonadorelin, angiotensin II) good linearity and reproducibility is observed in the 20 to 100 ng/mL concentration range. For the anionic peptides (N-t-Boc-Pentagastrin and two related peptides), a tITP method was developed using a dynamically coated capillary. The coating was prepared by adding Fluorad FC-135 to the leading electrolyte buffer. In this way a positively charged bilayer was formed on the inside of the capillary, producing an electroosmotic flow towards the outlet using reversed polarity conditions. In this way, acceptable analysis times were achieved. Using the developed tITP method, up to 72% of the capillary can be filled with sample solution as well. The anionic peptides are separated even better than when using CZE conditions. Linearity and reproducibility in the 20-100 ng/mL range proved to be excellent.

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Section: Stacking Of Anionic Compoundsmentioning

confidence: 98%

Development and validation of transient isotachophoretic capillary zone electrophoresis for determination of peptides

et al. 1998

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“…The capillary was filled with the leading electrolyte (L) and the sample was then injected hydrodynamically. As explained in the bibliography [10,24,25], the maximum amount of sample that can be injected into ITP systems is 55%, because injections larger than this cause band broadening. While the inlet vial contained T and the outlet vial contained L the analytes were focused on-column by applying a negative voltage in conjunction with a positive hydrodynamic back-pressure.…”

Section: Capillary Isotachophoresiscapillaryzone Electrophoresismentioning

confidence: 99%

“…The mobility of the sample ions is, therefore, intermediate and ions which are more mobile than the leading ions or less mobile than the terminating ions are removed from the capillary. This system cleans the sample substantially [24]. It is, therefore, important to choose a suitable electrolyte system, i. e. one in which stable isotachophoretic zones are formed and the analytes are completely separated.…”

Section: Capillary Isotachophoresiscapillaryzone Electrophoresismentioning

confidence: 99%

“…The electric field was therefore stronger in the sampling zone than in the separation zone, because the concentration of ions was lower. We controlled this step by monitoring the electric current [24]. The applied voltage was removed when the electric current generated was 95% of that generated when the capillary was full of BGE.…”

Section: Field-amplified Sample Injectioncapillaryzone Electraphoresismentioning

confidence: 99%

“…Finally, because of large variations in the composition of the sample matrix, a pretreatment step was likely to improve both the reproducibility and the efficiency of CZE analysis [24]. So, before using ITP or FASI the sample must be pretreated; in this work the sample was cleaned on Oasis HLB cartridges.…”

Section: Introductionmentioning

confidence: 99%

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Study of on-line stacking procedures for analyzing marbofloxacin by capillary zone electrophoresis

2002

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SummaryThis paper describes the analysis of marbofloxacin at low levels in pig plasma by capillary electrophoresis (CE). To increase the sensitivity of CE we studied I',,vo on-column stacking systems, field-amplified sample injection (FASI) and isotachophoresis (ITP), and optimized several conditions to obtain the best sensitivity with each. The best results were obtained by use of ITP-CZE with 10 mM sodium monohydrogen phosphate (pH 9.0) as the leading and background electrolytes and 10 mM/?-alanine (pH 9.0) as the terminating electrolyte. This system was 75 times more sensitive than conventional CE -the enhanced detection limit (LOD) was 20 Ixg kg 1.. A sample of pig plasma was analyzed after clean-up by solid-phase extraction on Oasis HLB cartridges and elution with methanol. Recoveries were approximately 100%.

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Comparison between transient isotachophoretic capillary zone electrophoresis and reversed-phase liquid chromatography for the determination of peptides in plasma

et al. 1999

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Strategy for setting up single-capillary isotachophoresis—zone electrophoresis

Cited by 54 publications

References 21 publications

Development and validation of transient isotachophoretic capillary zone electrophoresis for determination of peptides

Development and validation of transient isotachophoretic capillary zone electrophoresis for determination of peptides

Study of on-line stacking procedures for analyzing marbofloxacin by capillary zone electrophoresis

Comparison between transient isotachophoretic capillary zone electrophoresis and reversed-phase liquid chromatography for the determination of peptides in plasma

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