Comparison between transient isotachophoretic capillary zone electrophoresis and reversed-phase liquid chromatography for the determination of peptides in plasma

Waterval, Joop C. M.; Krabbe, Hans; Teeuwsen, J.; Bult, A.; Lingeman, H.; Underberg, W.J.M.

doi:10.1002/(sici)1522-2683(19991001)20:14<2909::aid-elps2909>3.0.co;2-m

Cited by 22 publications

(13 citation statements)

References 31 publications

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“…ITP has been used on-line to enhance the limit of detection for CZE-MS [17]. The sensitivity of ITP-CZE has been found comparable to that of LC for the analysis of peptide drugs in plasma [18]. ITP analysis has been combined with NMR detection both in continuous-flow and stopped-flow modes [19].…”

Section: Preconcentrationmentioning

confidence: 99%

Capillary electrophoresis of proteins 1999-2001

Dolnı́k¹,

Hutterer²

2001

Electrophoresis

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This review article with 223 references describes recent developments in capillary electrophoresis (CE) of proteins and covers papers published during last two years, from the previous review (V. Dolnik, Electrophoresis 1999, 20, 3106-3115) through Spring 2001. It describes the topics related to CE of proteins including modeling of the electrophoretic properties of proteins, sample pretreatment, wall coatings, improving selectivity, detection, special electrophoretic techniques, and applications.

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Section: Preconcentrationmentioning

confidence: 99%

Capillary electrophoresis of proteins 1999-2001

Dolnı́k¹,

Hutterer²

2001

Electrophoresis

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“…three orders of magnitude in comparison with normal CZE. tITP has been utilized for the determination of both peptides [12][13][14] and proteins [15]. Today, with tITP a concentration sensitivity about the same as in conventional HPLC can be reached.…”

Section: Electrophoresis-based Preconcentrationmentioning

confidence: 99%

On-line sample preconcentration in capillary electrophoresis, focused on the determination of proteins and peptides

Stroink

Paarlberg

Waterval³

et al. 2001

Electrophoresis

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This overview highlights the possibilities of on- or in-line preconcentration procedures in combination with a CZE separation, focused on the determination of peptides and proteins. The discussed methods, including sample stacking, field-amplified injection, isotachophoresis, solid phase extraction, membrane preconcentration, electroextraction, supported liquid membranes, hollow fibers, immunoaffinity, and molecularly imprinted polymers technology preconcentration are categorized in electrophoresis-based and chromatography-based preconcentration. The chromatography-based preconcentration is subdivided in low-specificity and high-specificity methods. A number of preconcentration methods are available, however, this paper demonstrates that various compounds in different media (aqueous solutions, urine, and plasma) require different preconcentration systems. The preconcentration techniques of first choice in general seem to be solid-phase extraction and membrane preconcentration, because of their high concentration ability, multiapplicability, relative simplicity and clean-up capability. For the future, hollow fibers seem to hold a great potential as preconcentration technique, yielding high concentration factors, using simple designs. New techniques, such as hollow fibers, molecularly imprinted polymers technology and supported liquid membranes may have the potential to supersede the conventional preconcentration techniques in some cases. The larger the arsenal of preconcentration techniques becomes, the more efficiently peptides and proteins may be analyzed in the future. These techniques, in some cases, require pre-cleanup procedures, to ensure the purity of the samples to concentrate.

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“…As sample preparation technique an SPE procedure is employed, based on a cation-exchange mechanism. This SPE procedure is earlier, in somewhat modified form, efficiently applied as sample preparation procedure prior to transient isotachophoresis CE [18] and liquid chromatography with fluorescence detection [37].…”

Section: On-capillary Preconcentrating Ce-uvmentioning

confidence: 99%

“…However, CE has intrinsic poor concentration sensitivity, due to a low loading capacity. Several solutions have been brought up to increase the sample loadability without loss of resolution, such as field-amplified sample stacking [2][3][4][5][6], transient isotachophoresis [7][8][9][10][11][12][13][14][15][16][17][18][19], and on-capillary preconcentration on a stationary phase [20][21][22][23][24][25][26][27][28][29][30][31]. The on-capillary preconcentration has the benefit that sample loading is not limited to the capillary volume and that on-line purification of the applied sample can be accomplished by employing washing steps.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoretic bioanalysis of therapeutically active peptides with UV and mass spectrometric detection after on-capillary preconcentration

et al. 2001

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An earlier developed capillary electrophoresis (CE) system with an on-capillary adsorptive phase is investigated for its suitability to quantitate low concentrations of angiotensin II and gonadorelin in plasma. An off-line solid-phase extraction is used for sample preparation. The on-line preconcentration CE system allows multiple capillary volumes of sample solution to be injected, increasing the concentration sensitivity of CE with 3-4 orders of magnitude. Furthermore, possible influence of matrix salts can be ruled out by employing a rinsing step after sample application. Using short-wavelength UV detection, reproducibility and linearity in the low nanomolar range were satisfactory. The capillary could be efficiently regenerated using a programmed between-run rinsing procedure, allowing 20-30 large injections of sample extracts. Coating of the capillary improved the robustness of the method. Mass spectrometric detection via a previously reported sheathless interface increased the selectivity and sensitivity substantially. Recommendations are provided for the sample preparation process, the most critical part of the system. Further purification of the sample is required to allow the loading of larger sample volumes and to optimize the system's robustness.

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Comparison between transient isotachophoretic capillary zone electrophoresis and reversed-phase liquid chromatography for the determination of peptides in plasma

Cited by 22 publications

References 31 publications

Capillary electrophoresis of proteins 1999-2001

Capillary electrophoresis of proteins 1999-2001

On-line sample preconcentration in capillary electrophoresis, focused on the determination of proteins and peptides

Capillary electrophoretic bioanalysis of therapeutically active peptides with UV and mass spectrometric detection after on-capillary preconcentration

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