Strain Variation in
            <i>Mycobacterium marinum</i>
            Fish Isolates

Ucko, Michal; Colorni, A.; Kvitt, Hagit; Diamant, Arik; Zlotkin, Amir; Knibb, Wayne

doi:10.1128/aem.69.1.724.2003

Cited by 30 publications

(52 citation statements)

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“…nov. These and previous observations (Chemlal et al, 2002;Ucko et al, 2002;Rhodes et al, 2003) Although IS2606 has been reported in Mycobacterium lentiflavum (Stinear et al, 1999), the presence of both IS2404 and IS2606 is considered specific to Mycobacterium ulcerans. Recently, both insertion sequences were identified in a mycobacterium isolated during an infection of laboratory-reared frogs that was characterized as being intermediate between Mycobacterium ulcerans and Mycobacterium marinum (Trott et al, 2004).…”

Section: Characteristic Percent Positivesupporting

confidence: 69%

“…On the basis of 16S rRNA and hsp65 gene sequences, L15 T is very similar to scotochromogenic species isolated from Mediterranean fish and described as strains of Mycobacterium marinum by Ucko et al (2002), who postulated that Mycobacterium marinum isolates have genotypes that are unique and specific to a geographical region. The 401 nt sequence of the L15 T hsp65 gene PCR product was identical to that of 'Mycobacterium seriolae', a photochromogenic mycobacterium isolated from maricultured yellowtail (Seriola quinqueradiata) in Japan (Kusuda et al, 1987), and a Mycobacterium ulcerans-like non-chromogenic mycobacterium isolated from a laboratory colony of frogs (Xenopus tropicalis) and which is also positive for IS2404 and IS2606 (Trott et al, 2004).…”

Section: Characteristic Percent Positivementioning

confidence: 92%

“…Sequences deposited in GenBank for a Mycobacterium ulcerans-like organism (AY500839) (Trott et al, 2004) and Mycobacterium marinum strains eilaticum DL240490 (AF456468), hellenicum DL045 (AF456471) and cyprinum CC240299 (AF456469) (Ucko et al, 2002) were identical to that of L15 T . Mycobacterium ulcerans (AY550209 and AF547888) differed by 1 or 3 nt and Mycobacterium marinum (AF547855) differed by 2 nt from L15 T .…”

Section: Characteristic Percent Positivementioning

confidence: 99%

See 2 more Smart Citations

Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)

Rhodes

Kator

McNabb

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

131

143

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Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis) A group of slowly growing photochromogenic mycobacteria was isolated from Chesapeake Bay striped bass (Morone saxatilis) during an epizootic of mycobacteriosis. Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those of the genus Mycobacterium. Biochemical reactions, growth characteristics and mycolic acid profiles (HPLC) resembled those of Mycobacterium shottsii, a non-pigmented mycobacterium also isolated during the same epizootic. Sequencing of the 16S rRNA genes, the gene encoding the exported repeated protein (erp) and the gene encoding the 65 kDa heat-shock protein (hsp65) and restriction enzyme analysis of the hsp65 gene demonstrated that this group of isolates is unique. Insertion sequences associated with Mycobacterium ulcerans, IS2404 and IS2606, were detected by PCR. These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 6C, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid (1 mg ml "1 ), p-nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide. On the basis of this polyphasic study, it is proposed that these isolates represent a novel species, Mycobacterium pseudoshottsii sp. nov. The type strain, L15 T , has been deposited in the American Type Culture Collection as ATCC BAA-883 T and the National Collection of Type Cultures (UK) as NCTC 13318 T .

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Section: Characteristic Percent Positivesupporting

confidence: 69%

Section: Characteristic Percent Positivementioning

confidence: 92%

Section: Characteristic Percent Positivementioning

confidence: 99%

See 1 more Smart Citation

Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)

Rhodes

Kator

McNabb

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

131

143

View full text Add to dashboard Cite

show abstract

“…However, few studies have employed this method for identification or for taxonomic relationships of aquatic mycobacteria. Ucko et al (16) used sequences of almost the entire 16S rRNA gene to characterize and seek heterogeneity of M. marinum strains isolated in Israel and other geographical regions. Furthermore, sequence analysis of this gene has been used to analyse several novel aquatic mycobacteria species (21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of sequence analysis of 16S rRNA and rpoBgenes for identification of aquatic mycobacteria

Pourahmad

Richards²

2016

Turk J Vet Anim Sci

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“…En Israel la micobacteriosis es una de las enfermedades más devastadoras en los recientes años (32). Conroy (18) reporta importantes infecciones con micobacteriosis en el cultivo de tilapia en países africanos, en la región de América Central y en Cuba.…”

Section: Discussionunclassified

Determinación y prevalencia de Mycobacterium spp., en tilapia nilótica (Oreochromis niloticus) cultivada en Campeche, México

et al. 2013

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RESUMENObjetivo. Determinar la presencia y prevalencia de Mycobacterium spp., en granjas de tilapia nilótica (Oreochromis niloticus) en el Municipio de Champotón, Campeche, México. Materiales y métodos. La colecta de organismos se realizó en tres granjas de cultivo de tilapia nilótica del municipio de Champotón, Campeche, México. Los organismos se examinaron externa e internamente y se tomó una muestra de riñón la cual fue sembrada en forma de estría en medios de cultivo: Löwesntein-Jensen, TCBS, KF y en TSA; las placas fueron incubadas a 35°C de 24 a 48 horas, los órganos fueron fijados en formalina tamponada al 10% para ser procesados para histología de rutina para análisis posteriores. Asimismo, muestras de cultivo bacteriológico y de tejido fueron teñidas con la técnica de Ziel-Neelsen con el fin de observar la presencia de bacilos ácido-alcohol resistentes. Resultados. Los resultados obtenidos sugieren que la presencia de Mycobacterium spp., es constante y en alta prevalencia y puede ser un factor que este mermando la rentabilidad del cultivo. Conclusiones. La presencia de Mycobacterium spp., representa un riesgo para el cultivo de tilapia en el municipio de Champotón, por ser una enfermedad muy persistente y difícil de erradicar una vez ocurrido e brote de infección, por lo cual es importante llevar estudios más detallados de la presencia de este género bacteriano, así como, medidas de prevención y dispersión de este patógeno en los cultivos adyacentes. ABSTRACTObjective. Determine the presence and prevalence of Mycobacterium spp., at tilapia (Oreochromis niloticus) farms in the municipality of Champotón, Campeche, Mexico. Materials and methods. The collection of organisms was conducted in three farms of Nile tilapia at Champotón township, Campeche, Mexico. The organisms were examined externally and internally and a kidney sample was inoculated in different growth media: Löwestein-Jensen, TCBS, KF and TSA. The plates were incubated at 35°C for 24 to 48 hours, the organs were fixed in 10% buffered formalin to be processed for routine histology for further analysis. Likewise, bacterial culture samples and tissue were stained through the Ziel-Neelsen technique to observe the presence of acid-fast bacilli. Results. The results suggest that the presence of Mycobacterium spp., is constant and its high prevalence and could be an undermining issue in the profitability of this culture. Conclusions. The presence of Mycobacterium spp., represents a risk for tilapia culture in the municipality of Champotón, it is a very persistent and difficult to eradicate disease once an outbreak has occurred, reason for which it is important to bring more detailed studies of the presence of this bacterial genus, as well as prevention and spread of this pathogen in adjacent cultures.

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Strain Variation in Mycobacterium marinum Fish Isolates

Cited by 30 publications

References 0 publications

Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)

Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)

Comparative evaluation of sequence analysis of 16S rRNA and rpoBgenes for identification of aquatic mycobacteria

Determinación y prevalencia de Mycobacterium spp., en tilapia nilótica (Oreochromis niloticus) cultivada en Campeche, México

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