Storage of PBSC at -80°C

Watts, Mike; Sullivan, Aideen M.; Ings, Stuart J.; Barlow, Maeve; Devereux, Stephen; Goldstone, AH; Linch, DC

doi:10.1038/sj.bmt.1701050

Cited by 9 publications

(10 citation statements)

References 5 publications

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“…Several authors have described the biological characteristics of PBSC stored in − 80°C mechanical freezers. Several studies 15,22 showed quantitative and functional loss of PBSC stored beyond 6 months, whereas other studies 5,17,23,25,26 observed no correlation between PBSC storage duration and recovery even 5 years after freezing. Moreover, only few publications have investigated the influence of long-term storage at − 80°C on haematopoietic reconstitution following the transplantation of PBSC.…”

Section: Discussionmentioning

confidence: 99%

“…The median time required to reach 0.5 × 10 9 /L neutrophils was 12 (1-43), 11 (7-28) and 11 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) days in groups I, II and III, respectively. We did not observe any negative impact of storage duration on neutrophil recovery.…”

Section: Haematopoietic Reconstitutionmentioning

confidence: 99%

“…However, although PBSC cryopreservation using liquid nitrogen and controlled-rate freezing is suitable for long-term storage (more than 10 years [19][20][21] ), the reliability of long-term PBSC storage at − 80°C is still not clear. Several studies have shown quantitative and qualitative losses in PBSC stored for longer than 6 months, 15,22 whereas other studies have reported no significant change in post-thawing recovery parameters including cell viability, CD34 + cells, burst forming uniterythroid or colony forming unit-granulocyte macrophage. 5,23 The short-term haematopoietic reconstitution of PBSC kept at − 80°C was comparable with that observed after storage in liquid nitrogen but was only assessed after storage for 4 months, 6 7 months 8,15 or up to 23 months.…”

Section: Introductionmentioning

confidence: 99%

“…5,23 The short-term haematopoietic reconstitution of PBSC kept at − 80°C was comparable with that observed after storage in liquid nitrogen but was only assessed after storage for 4 months, 6 7 months 8,15 or up to 23 months. 22 To evaluate the impact of long-term storage on haematopoietic recovery after both uncontrolled-rate freezing and cryopreservation at − 80°C, we combined engraftment clinical data from 666 autologous PBSC transplantations (PBSCTs) performed in two haematology departments, one from France, and one from Belgium.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Impact of uncontrolled freezing and long-term storage of peripheral blood stem cells at −80 °C on haematopoietic recovery after autologous transplantation. Report from two centres

Detry

Calvet²,

Straetmans

et al. 2014

Bone Marrow Transplant

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Controlled-rate freezing and storage in vapour phase nitrogen are used by most transplantation teams for the cryopreservation and storage of peripheral blood haematopoietic stem cells (PBSC). In this study, we analysed 666 autologous PBSC transplants after uncontrolled freezing and storage of PBSC at − 80°C. Statistical analysis showed that neutrophil recovery was associated with both the infused CD34 + cell dose (P = 0.01) and the post transplantation use of growth factors (P o 0.001) and that platelet recovery was associated with the infused CD34 + cell dose (P o0.001) and with the diagnosis (P = 0.02). We analysed three groups according to the duration of the cryopreservation period (less than 6 months, between 6 and 12 months or more than 1 year). Haematopoietic recovery was not found to be adversely affected by longer storage at − 80°C. The haematopoietic recoveries of 50 pairs of sequential transplantations from the same PBSC mobilization were analysed. Despite prolonged cryopreservation, there were no statistically significant differences in neutrophil (P = 0.09) or platelet (P = 0.22) recovery in the second compared with the first transplant. In conclusion, the long-term storage of PBSC at − 80°C after uncontrolled-rate freezing is an easy and comparatively inexpensive cryopreservation method that leads to successful haematopoietic recovery even after prolonged storage.

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Section: Discussionmentioning

confidence: 99%

Section: Haematopoietic Reconstitutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of uncontrolled freezing and long-term storage of peripheral blood stem cells at −80 °C on haematopoietic recovery after autologous transplantation. Report from two centres

Detry

Calvet²,

Straetmans

et al. 2014

Bone Marrow Transplant

View full text Add to dashboard Cite

show abstract

“…10 For longer-term storage (years) cryopreservation with a final concentration of 10% DMSO and storage in liquid or vapor phase nitrogen is still the preferred and most widely used methodology, as there has been some indication of a fall in GM-CFU (granulocyte macrophage colony-forming unit) colonies after 1 year of storage at − 80°C. 11 Protection from temperature fluctuations is an important consideration. Products stored in liquid-phase LN 2 (−196°C) are better protected from temperature fluctuations but are at increased risk for bag breakage at thaw and cross-contamination from other products.…”

Section: Personnelmentioning

confidence: 99%