Storage conditions of avulsed teeth affect the phenotype of cultured human periodontal ligament cells

Abstract

After severe injury to the periodontal ligament (PL), the phenotypes of cells recolonizing root surfaces influence the extent and type of repair processes. In teeth that are replanted following avulsion injury, recolonization of the PL space by osteogenic cells instead of by PL fibroblasts may favor bone formation (i.e. ankylosis) instead of PL regeneration. We consider here that recolonization processes depend in part on the storage conditions of the teeth following avulsion. We used an in vitro cell culture model to assess the effect of storage conditions on immunohistochemical staining of several marker proteins that are expressed by osteogenic cells (osteopontin and alkaline phosphatase) and fibroblasts (α‐smooth muscle actin, type III and XII collagens). Prior to cell culture, extracted human premolar teeth were stored in air (“dry”) or in α‐MEM (“wet”) for either 30 or 120 min as surrogate conditions for the variations of extra‐alveolar tooth storage that may occur following avulsion. Collagenase/trypsin‐digested suspensions of PL cells were prepared from the tissue adherent to the extracted root surface. Passage #2 or #3 cultures were immunostained and examined by fluorescence microscopy. For type XII collagen, cells from wet samples displayed perinuclear staining while cells from 30‐min dry samples showed only isolated foci. The staining for 120‐min dry samples was weak and non‐specific. α‐Smooth muscle actin was not incorporated into stress fibers in wet samples, whereas dry samples demonstrated prominent stress fibers stained for α‐smooth muscle actin. Detached cytoplasmic fragments resembling cell processes that stained for α‐smooth muscle actin were abundant in dry samples, indicating the presence of highly contractile cells. The staining for osteopontin was mainly perinuclear but was more intense in dry samples. The focal adhesion pattern of osteopontin staining in 120‐min dry samples resembled that of migrating osteogenic cells. The pattern of staining did not vary for type III collagen or alkaline phosphatase, although staining for alkaline phosphatase was more intense in samples stored under dry conditions. We conclude that prolonged extra‐alveolar dry storage favors increased in vitro growth of contractile cells expressing osteogenic cell markers while storage in cell culture medium favors growth of cells with the classical phenotype of PL fibroblasts.

Regulatory mechanisms of periodontal regeneration

“…In contrast, only a few investigations on the precise mechanisms have been reported with regard to the relationship between tooth implantation or replantation and regeneration of the periodontal ligament (Iqbal and Bamaas, 2001;Kawanami, et al, 2001;Lekic et al, 2001;Lin et al, 2000).…”

Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth

“…To date, most replantation studies have focused on factors such as extraoral period, storage media for tooth, timing of endodontic treatment, duration of root canal medication and development of healing complications (Hiremath and Kidiyoor 2011; Lekic et al 1998; Lin et al 2000). Andreasen reported that the decisive factor for PDL healing was immediate replantation (Andreasen et al 1995) indicating that if the teeth are not replanted immediately, they will most probably be lost due to root resorption or infraocclusion (Andreasen et al 1995).…”

The Adipose-derived Stem Cell: Looking Back and Looking Ahead

“…Of course, we now know these cells to be hematopoietic stem cells (HSCs), the first described adult stem cell, although one could argue for the muscle-derived satellite cell described in 1961 to take this title (Mauro, 1961; Moss and Leblond, 1971). From the work of McCulloch and Till, the adult stem cell field gathered momentum in the late sixties and early seventies as patients suffering from SCID (severe combined immunodeficiency) were treated using bone marrow transplants or HSC concentrates (Dicke and van Bekkum, 1973; Lin et al , 2000). In 1978, HSCs were identified in umbilical cord blood and a new population of adult stem cells was born: the umbilical cord blood stem cell (Emerson et al , 1985; Broxmeyer et al , 1990).…”