Stimulatory Effects of Low-concentration Reactive Oxygen Species on Calcification Ability of Human Dental Pulp Cells

Matsui, Satoshi; Takahashi, Chitaka; Tsujimoto, Yasuhisa; Matsushima, Kiyoshi

doi:10.1016/j.joen.2008.08.034

Cited by 40 publications

(56 citation statements)

References 25 publications

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“…Low concentrations of H 2 O 2 increase ALP activity and potentiate the progression of aortic valve calcification [22,23] . The levels of expression of mRNAs for OPN and OCN were significantly greater in ROS-treated human dental pulp cells [24] . Thus, rather than simply causing oxidative damage, ROS played a more complex role in cell physiology.…”

Section: Discussionmentioning

confidence: 87%

Oxidized low-density lipoprotein and β-glycerophosphate synergistically induce endothelial progenitor cell ossification

Liu

Chen

et al. 2011

Acta Pharmacol Sin

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Aim: To investigate the ability of ox-LDL to induce ossification of endothelial progenitor cells (EPCs) in vitro and explored whether oxidative stress, especially hypoxia inducible factor-1α (HIF-1α) and reactive oxygen species (ROS), participate in the ossific process. Methods: Rat bone marrow-derived endothelial progenitor cells (BMEPCs) were cultured in endothelial growth medium supplemented with VEGF (40 ng/mL) and bFGF (10 ng/mL). The cells were treated with oxidized low-density lipoprotein (ox-LDL, 5 µg/mL) and/or β-glycerophosphate (β-GP, 10 mmol/L). Calcium content and Von Kossa staining were used as the measures of calcium deposition. Ossific gene expression was determined using RT-PCR. The expression of osteocalcin (OCN) was detected with immunofluorescence. Alkaline phosphatase (ALP) activity was analyzed using colorimetric assay. Intercellular reactive oxygen species (ROS) were measured with flow cytometry. Results: BMEPCs exhibited a spindle-like shape. The percentage of cells that expressed the cell markers of EPCs CD34, CD133, and kinase insert domain-containing receptor (KDR) were 46.2%±5.8%, 23.5%±4.0%, and 74.3%±8.8%, respectively. Among the total cells, 78.3%±4.2% were stained with endothelial-specific fluorescence. Treatment of BMEPCs with ox-LDL significantly promoted calcium deposition, which was further significantly enhanced by co-treatment with β-GP. The same treatments significantly increased the gene expression of core-binding factor a-1 (cbfa-1) and OCN, while decreased the gene expression of osteoprotegerin (OPG). The treatments also significantly enhanced the activity of ALP, but did not affect the number of OCN + cells. Furthermore, the treatments significantly increased ROS and activated the hypoxia inducible factor-1α (HIF-1α). In all these effects, ox-LDL acted synergistically with β-GP. Conclusion: Ox-LDL and β-GP synergistically induce ossification of BMEPCs, in which an oxidizing mechanism is involved.

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Section: Discussionmentioning

confidence: 87%

Oxidized low-density lipoprotein and β-glycerophosphate synergistically induce endothelial progenitor cell ossification

Liu

Chen

et al. 2011

Acta Pharmacol Sin

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“…A reduction in the HP concentration in bleaching gels (15%-17.5%) also decreases the: (1) HP diffusion through enamel and dentin [15,25]; (2) pulp cell toxicity [15]; and (3) clinical tooth sensitivity [26]. In addition, mineralization activity is up-regulated when cultured pulp cells are exposed to low concentrations of HP [28][29][30]. It is known that decreasing the concentration of HP in the bleaching gels or reducing the application time of these products to enamel does not cause an immediate visual (esthetic) impact, as observed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Histopathological Features of Dental Pulp Tissue from Bleached Mandibular Incisors

Roderjan¹,

Stanislawczuk²,

Hebling³

et al. 2014

JMSE-B

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Mandibular incisors scheduled for extraction were subjected or not to three 15-minute applications of a 35% hydrogen peroxide gel. Two days thereafter, bleaching effectiveness (according to a value-oriented shade guide) was evaluated, and histological analysis of pulp tissue was performed. Immediately and two days after bleaching, tooth sensitivity (TS) experience was recorded. Bleached teeth of both groups showed significant color improvement (Mann-Whitney; p < 0.05). However, these teeth presented significant pulp alterations when compared with non-bleached teeth (Mann-Whitney; p < 0.05), including areas of superficial necrosis associated with mild inflammatory reactions. These data were achieved by recordings of TS immediately after bleaching (Fisher's exact test; p<0.05), which was reduced with time. Instead of 'no significant difference' being observed between young and old teeth (Mann-Whitney; p<0.05), necrosis occurred in 60% of old bleached teeth in comparison with 100% of young teeth, associated with underlying tertiary dentin deposition. It was concluded that, regardless of the age of patients, the application of a 35%-HP bleaching gel to mandibular incisors causes pulp damage, which was related to initial TS; however, superficial necrosis was more prevalent in young teeth.

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“…Therefore, when they are lost, reparative dentinogenesis takes place, which involves mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation into odontoblast-like cells, resulting in the deposition of mineralized matrix and restoration of the homeostasis of pulp tissue. [5][6][7] It was previously demonstrated that very low concentrations of H 2 O 2 induce odontoblastic differentiation and mineralized matrix deposition 8,9 ; conversely, toxic concentrations of H 2 O 2 enhance inflammatory mediator expression associated with the impairment of odontoblastic differentiation capability. [10][11][12][13][14] Recently, it was demonstrated that application of a lowconcentration H 2 O 2 gel (17.5%) from 5 to 45 min onto dental structure was able to reduce in about 11.3-4.5 times, respectively, the indirect toxicity of in-office tooth-bleaching to human dental pulp cells (HDPCs), when compared with traditional therapy (35% H 2 O 2 ; 3 Â 15 min).…”

Section: Introductionmentioning

confidence: 99%

Responses of human dental pulp cells after application of a low-concentration bleaching gel to enamel

Soares

Basso

Scheffel

et al. 2015

Archives of Oral Biology

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Stimulatory Effects of Low-concentration Reactive Oxygen Species on Calcification Ability of Human Dental Pulp Cells

Cited by 40 publications

References 25 publications

Oxidized low-density lipoprotein and β-glycerophosphate synergistically induce endothelial progenitor cell ossification

Oxidized low-density lipoprotein and β-glycerophosphate synergistically induce endothelial progenitor cell ossification

Histopathological Features of Dental Pulp Tissue from Bleached Mandibular Incisors

Responses of human dental pulp cells after application of a low-concentration bleaching gel to enamel

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