Stimulatory effect of engineered three-layer adipose tissue-derived stem cells sheet in atelocollagen matrix on wound healing in a mouse model of radiation-induced skin injury

Abstract

To investigate the effectiveness of adipose tissue-derived stem cells sheet combined with atelocollagen matrix on a mouse model of radiation-induced skin injury, adipose tissue-derived stem cells were harvested from the bilateral groin of mice and were screened out by fluorescence-activated cell sorting. Cell sheets were created on a temperature-responsive cell culture surface. The wound recovery rate was detected by measuring the injury area and the recovery shapes of wound were monitored by hematoxylin–eosin, Masson’s trichrome, and immunohistochemical staining. Quantitative reverse transcription polymerase chain reaction and Western blot analysis were conducted to detect relative mRNA expression level of interleukin-1β and protein expression level of vascular endothelial growth factor, respectively. As results, the three-layer adipose tissue-derived stem cells sheet combined with atelocollagen matrix treatment promoted wound healing significantly compared to other treatments. More collagen, higher vessel density, and milder inflammation were observed in the group of three-layer adipose tissue-derived stem cells sheet combined with atelocollagen matrix compared with other treatments. Moreover, both mRNA expression level of interleukin-1β and protein expression level of vascular endothelial growth factor were drastically decreased in that group. In conclusion, three-layer adipose tissue-derived stem cells sheet combined with atelocollagen matrix could promote wound healing synergistically, providing a substantial advance in cell-based tissue engineering and wound healing.

SIRT3 deficiency delays diabetic skin wound healing via oxidative stress and necroptosis enhancement

“…The process of skin trauma is essentially the damage by imbalance of skin internal environment with harmful stimulation as oxidative stress, inflammation, radiation etc During wound healing, various inflammatory cells, such as macrophages, neutrophils, even endothelial cells and fibroblasts, produce active oxygen and free radicals . Although appropriate amount of free radicals are beneficial to promote wound healing, too much active oxygen suppresses the migration and proliferation of repair cells, inhibits extracellular matrix synthesis and finally delays wound healing .…”

Adipose-Derived Stromal Cells for Chronic Wounds: Scientific Evidence and Roadmap Toward Clinical Practice

“…Scaffolds should possess several features, such as bio-compatibility, non-toxicity, resemblance to the ECM and optimal porosity to support ASC growth and activity. Many scaffolds combined with ASC have been tested on wounds: chitosan, 39 fibrin, 40 hyaluronic acid, 41 collagen sponge, 42 collagen peptide scaffolds, 43 decellularized tissues, 44 , 45 atelocollagen, 46 , 47 amniotic membranes, 48 platelet gels, 49 and mixtures of different scaffolds. 50 These biomaterials are not only physical 3D support but can be biologically active on wounds and/or ASC, 51 resulting in improved healing properties, regulations of proliferation, adhesion, and the secretome composition.…”

Stem Cell-Based Tissue Engineering for the Treatment of Burn Wounds: A Systematic Review of Preclinical Studies

“…Radiation burns are clinically far less common than thermal, electrical and chemical burns, with very different pathophysiological responses. Only 2 of the included studies used radiation burn in their animal models [ 100 , 101 ]. Compared to thermal skin burns, radiation burns are characterised by necrosis, paroxysmal and chronic pain resistant to opiates, as well as uncontrolled, successive inflammatory waves [ 102 ].…”

“…However, unlike for bone marrow and adipose tissue, ready access to umbilical cord as a stem cell source is more limited. While these are the predominant sources of stem cells considered for Acellular dermal matrix [164,175] Alginate [167,181] Atelocollagen [100] Chitosan [123,159,160,163,167,181] Collagen [160,161,171,173] [180] Decellularised bladder [138] Decellularised small intestine submucosa [166] Fibrin [72,161,162,165,168] Fibrinogen [178] Gelatin [71] Glycosaminoglycan [173,179] Hyaluronic acid [178] Platelet-rich plasma [176] Silk fibroin [172] Sodium carboxymethyl cellulose [73] Thrombin [178] Arginine-based polyester amide [123] Polycaprolactone [163] Poly-D,L-lactic acid [89,170] Polyethylene glycol [72,161,162,176] Polyketone [174] Polyvinyl alcohol [159,163,173] Integra® [74] Dermal ...…”