Stimulation of wound healing by helium atmospheric pressure plasma treatment

Abstract

New experiments using atmospheric pressure plasma have found large application in treatment of living cells or tissues, wound healing, cancerous cell apoptosis, blood coagulation on wounds, bone tissue modification, sterilization and decontamination. In this study an atmospheric pressure plasma jet generated using a cylindrical dielectric-barrier discharge was applied for treatment of burned wounds on Wistar rats' skin. The low temperature plasma jet works in helium and is driven by high voltage pulses. Oxygen and nitrogen based impurities are identified in the jet by emission spectroscopy. This paper analyses the natural epithelization of the rats' skin wounds and two methods of assisted epithelization, a classical one using polyurethane wound dressing and a new one using daily atmospheric pressure plasma treatment of wounds. Systemic and local medical data, such as haematological, biochemical and histological parameters, were monitored during entire period of study. Increased oxidative stress was observed for plasma treated wound. This result can be related to the presence in the plasma volume of active species, such as O and OH radicals. Both methods, wound dressing and plasma-assisted epithelization, provided positive medical results related to the recovery process of burned wounds. The dynamics of the skin regeneration process was modified: the epidermis re-epitelization was accelerated, while the recovery of superficial dermis was slowed down.

Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways

“…NTAPP has also been reported to accelerate wound healing processes by activating the nuclear factor erythroid-related factor 2 (NRF2) signaling pathway in human keratinocyte HaCa T cell line in vitro 19, and to promote re-epithelialization and wound closure by activating keratinocytes and fibroblasts in Wistar rats’ wound skin20. These studies strongly suggested that NTAPP stimulates the proliferation of normal and adult stem cells.…”

Electrical, Thermal and Optical Parametric Study of Guided Ionization Waves Produced with a Compact μs-Pulsed DBD-Based Reactor

“…Although, the electrodes that are used therein are in the form of very thin rings, which are entirely enclosed in the dielectric material, thus avoiding any contact with the atmospheric air for better insulation. Besides, setups using one single electrode have been also proposed [19,[32][33][34][35][36], which in some cases, are directly compared with linear-field configurations in the same study [28]. In most of these setups, quartz is used as dielectric material and helium as the process gas, which are quite reliable.…”

Cold atmospheric pressure plasma for treatment of chronic wounds: drug or medical device?

“…[16][17][18][19][20][21] A plasma-activated medium has even been claimed to exert anti-tumor effects. 22,23 Results of in-vitro, 24 in-vivo [24][25][26][27][28] and initial clinical studies on chronic wounds of domestic animals 29,30 and humans [31][32][33][34][35] indicate that CAP may be a promising alternative to conventional treatment options, based on the following biological premises:…”