Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways

Park, Jeongyeon; Lee, Hyun‐Young; Lee, Hae June; Kim, Gyoo Cheon; Kim, Do Young; Han, Sungbum; Song, Kiwon

doi:10.1038/srep39298

Cited by 44 publications

(59 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The deployment of plasma medical procedures will likely require control mechanisms for the plasma source so that the desired or required reactants can be reliably delivered to the tissue, particularly in the case of liquid covered tissue [16]. Depending on the desired method of treatment, there may be different requirements to tailor the reactive oxygen and nitrogen species (RONS) penetrating through the liquid layer to the surface below [17][18][19]. The intrinsic variability of APPJs when used in a clinical setting (e.g.…”

Section: Introductionmentioning

confidence: 99%

Atmospheric pressure plasma jets onto a reactive water layer over tissue: pulse repetition rate as a control mechanism

Norberg¹,

Parsey²,

Lietz³

et al. 2018

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

The use of plasma jets to treat tissue in the context of plasma medicine often involves a thin intervening liquid layer on top of the tissue. Plasma activated species first transport through and react in the liquid layer prior to reaching the tissue. Of the many parameters that can be used to control this process, pulse repetition frequency (PRF) stands out. Results from a computational investigation of multiple pulses at varying PRF from an atmospheric pressure plasma jet (APPJ) onto a reactive liquid layer are discussed, and three key trends are made clear. First, a high PRF (short time between pulses) enables the gaseous species produced during the previous pulse to remain in the vicinity of the plasma at the onset of the next pulse, thereby increasing the inventory of (H)N x O y and O 3 in the gas phase. These species then solvate into the liquid, water in this case, and produce higher densities of aqueous ozone, nitrate, and peroxynitrite. With a lower PRF, reactants produced on a previous pulse are convected away prior to the next discharge pulse with more spatial separation of reactants both above and within the water. As a result, more of the hydroxyl anion (OH − aq), ozone anion (OH − 3 aq) and nitric oxide (NO aq) reach the tissue beneath the water. The second trend is that the production of H 2 O 2aq and its fluence to the underlying tissue are relatively independent of the PRF. The precursors for H 2 O 2aq are primarily produced by the surface ionization wave (SIW) on the top of the liquid, which then directly solvate into the liquid. Lastly, when the plasma plume touches the liquid, the SIW on the water layer increases the production of all aqueous species compared to configurations where the plasma plume does not touch the liquid. These trends are true for all PRF.

show abstract

Section: Introductionmentioning

confidence: 99%

Atmospheric pressure plasma jets onto a reactive water layer over tissue: pulse repetition rate as a control mechanism

Norberg¹,

Parsey²,

Lietz³

et al. 2018

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

show abstract

“…Those authors reported that stem cell proliferation was 1.57-fold higher as early as 72 h after CAP treatment. The study also reported the influence of NO induced by CAP [87]. This was confirmed by the CAP device's positive increase in stem cell proliferation being dramatically diminished by application of NO scavengers.…”

Section: Use Of Cap To Activate Proliferation In Stem and Progenitor mentioning

confidence: 66%

“…However, only a few analyses have focused on this possibility. One study by Park et al showed that CAP devices did actually induce the proliferation of stem cells derived from adipose tissue (ASC) without affecting their vital properties in any way [87]. Those authors reported that stem cell proliferation was 1.57-fold higher as early as 72 h after CAP treatment.…”

Section: Use Of Cap To Activate Proliferation In Stem and Progenitor mentioning

confidence: 99%

Cold Atmospheric Plasma: A Powerful Tool for Modern Medicine

Braný

Dvorska

Halašová

et al. 2020

IJMS

186

138

View full text Add to dashboard Cite

Cold atmospheric plasma use in clinical studies is mainly limited to the treatment of chronic wounds, but its application in a wide range of medical fields is now the goal of many analyses. It is therefore likely that its application spectrum will be expanded in the future. Cold atmospheric plasma has been shown to reduce microbial load without any known significant negative effects on healthy tissues, and this should enhance its possible application to any microbial infection site. It has also been shown to have anti-tumour effects. In addition, it acts proliferatively on stem cells and other cultivated cells, and the highly increased nitric oxide levels have a very important effect on this proliferation. Cold atmospheric plasma use may also have a beneficial effect on immunotherapy in cancer patients. Finally, it is possible that the use of plasma devices will not remain limited to surface structures, because current endeavours to develop sufficiently miniature microplasma devices could very likely lead to its application in subcutaneous and internal structures. This study summarises the available literature on cold plasma action mechanisms and analyses of its current in vivo and in vitro use, primarily in the fields of regenerative and dental medicine and oncology.

show abstract

“…Previous studies investigated the effects of non-thermal plasma on the attachment and proliferation of cells [13][14][15]. In a previous study that applied helium-based NTAPPJ to adipose-tissue-derived stem cells, more cells proliferated with the NTAPPJ exposure and their stem-like properties were unaffected [16]. Here, different gas sources were used for the NTAPPJ and the focus was on markers of stemness-namely, CD44 and CD105.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have indicated that a certain duration of exposure to plasma may have bioactive effects, such as improved cell attachment and proliferation [13][14][15]. A study that considered the use of adipose-tissue-derived stem cells (ASCs) indicated that there was an increase in the proliferation of cells by NTAPPJ exposure, which did not affect their stemness [16]. In another study in which the effect of NTAPPJ exposure was considered on human mesenchymal stem cells isolated from periodontal ligaments (hPDL-MSCs), it was evident that the hPDL-MSCs possessed all of the typical MSC properties, including the capacity for multilineage differentiation [17].…”

Section: Of 10mentioning

confidence: 99%

Effects of a Non-Thermal Atmospheric Pressure Plasma Jet with Different Gas Sources and Modes of Treatment on the Fate of Human Mesenchymal Stem Cells

et al. 2019

View full text Add to dashboard Cite

Despite numerous attempts to use human mesenchymal stem cells (hMSCs) in the field of tissue engineering, the control of their differentiation remains challenging. Here, we investigated possible applications of a non-thermal atmospheric pressure plasma jet (NTAPPJ) to control the differentiation of hMSCs. An air- or nitrogen-based NTAPPJ was applied to hMSCs in culture media, either directly or by media treatment in which the cells were plated after the medium was exposed to the NTAPPJ. The durations of exposure were 1, 2, and 4 min, and the control was not exposed to the NTAPPJ. The initial attachment of the cells was assessed by a water-soluble tetrazolium assay, and the gene expression in the cells was assessed through reverse-transcription polymerase chain reaction and immunofluorescence staining. The results showed that the gene expression in the hMSCs was generally increased by the NTAPPJ exposure, but the enhancement was dependent on the conditions of the exposure, such as the source of the gas and the treatment method used. These results were attributed to the chemicals in the extracellular environment and the reactive oxygen species generated by the plasma. Hence, it was concluded that by applying the best conditions for the NTAPPJ exposure of hMSCs, the control of hMSC differentiation was possible, and therefore, exposure to an NTAPPJ is a promising method for tissue engineering.

show abstract

Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways

Cited by 44 publications

References 88 publications

Atmospheric pressure plasma jets onto a reactive water layer over tissue: pulse repetition rate as a control mechanism

Atmospheric pressure plasma jets onto a reactive water layer over tissue: pulse repetition rate as a control mechanism

Cold Atmospheric Plasma: A Powerful Tool for Modern Medicine

Effects of a Non-Thermal Atmospheric Pressure Plasma Jet with Different Gas Sources and Modes of Treatment on the Fate of Human Mesenchymal Stem Cells

Contact Info

Product

Resources

About