Stimulation of cleavage in Arbacia eggs with desoxyribonucleic acid fractions

Butros, Joseph

doi:10.1016/0014-4827(59)90011-4

Cited by 9 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Their mechanisms of action and chemical structures are at present speculative. However, the importance of a DNA digest in relation to systems involved in DNA synthesis has been cited in several reports (Butros, 1959;Taliaferro and Jaroslow, 1960). As far as the antagonist is concerned, a literature survey has not revealed the existence of such a component.…”

Section: Discussionmentioning

confidence: 99%

Effects of Deoxyribonucleic Acid Products on Deoxyribonucleic Acid Synthesis of Virulent and Avirulent Pneumococci

Firshein

1961

J Bacteriol

View full text Add to dashboard Cite

FIRSHEIN, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococei synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the presence of DNA + deoxyribonuclease + mixtures of deoxynucleosides and deoxynucleotides (supplement-1) than unsupplemented suspensions. Under identical conditions, avirulent and weakly virulent pneumococci do not respond to these DNA breakdown products. Glucose and casitone must be present for maximal effects to occur. A DNA turnover occurs in virulent cells. This has been demonstrated by a decrease in specific activity of H3-DNA extracted from virulent (S) cells exposed to nonradioactive supplement-1.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Deoxyribonucleic Acid Products on Deoxyribonucleic Acid Synthesis of Virulent and Avirulent Pneumococci

Firshein

1961

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…04. ticed in studies of induction. Niu [1,2], Butros [3] and Benitez et al [4] first tested the ability of ribonucleoproteins and ribonucleic acids to enter the indeterminate embryonic cells and induce them to differentiate into a homogenetic, highly specific cell type.…”

Section: Introductionmentioning

confidence: 99%

Induction of Somites by Myosin mRNA

Bolzern¹,

Leonardi²,

Bernardi³

et al. 1983

Pathobiology

View full text Add to dashboard Cite

The effects of the messenger for myosin heavy chain (26S mRNA) on postnodal explants of chick embryo blastoderm were studied. Somites do not differentiate in the post-nodal explants of chick embryo cultivated by New’s method. They are induced when postnodal pieces are cultivated in the presence of a 26S mRNA extracted from chick leg muscles or in the presence of myosin. 26S mRNA plus actinomycin D induces small somites. 26S mRNA of duck, rabbit, or trout induce somite structures often built up of cells separated by large spaces and joined around a large myocele. Crayfish 26S mRNA or chick myosin light chain induce columnar cells connected around a cavity. Liver or kidney mRNA do not induce. The induction process can be summarized as follows: 26S mRNA codes for myosin (heavy chain) and the myosin (heavy chain) induces the somites. Induction of somites by mRNA can occur in the presence of actinomycin D but not when there is mRNA plus puromycin. It does occur when myosin acts in the presence of puromycin. Myosin and its heavy chain are present in chick blastoderm before the appearance of somites. Induced somites are able to induce neural plates. We conclude that in normal development somites are induced by myosin.

show abstract

“…We already have referred more specifically to the data of Hotta and Stern (39) and Takats (75). In addition, Butros (16) has observed a relationship between rates of cleavage of Arbacia eggs and the presence of specific DNA digests. A relationship between the levels of acid deoxyribonuclease in different tissues and species and rates of multiplication of the cells involved has been noted (18).…”

Section: Discussionmentioning

confidence: 94%