Biodynamic effects of oligonucleotides.

Braun, Werner; Firshein, W

doi:10.1128/mmbr.31.2.83-94.1967

Cited by 17 publications

(2 citation statements)

References 34 publications

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“…Thus, by the present study the mechanisms of the adjuvant effect of LPS were partly clarified at the level of host cells. Recently attention has been focused on the nucleic acids product as an effective adjuvant and many studies ascribed the adjuvant effect of LPS to these substances which might be released from host cells injured by the cytotoxicity of LPS [1][2][3]24]. The present study did not deal with this problem and further studies will be expected to correlate the present findings with this problem.…”

Section: Discussionmentioning

confidence: 80%

Cellular Mechanisms of Adjuvant Action of Bacterial Lipopolysaccharide in Anti‐Sheep Red Blood Cell Antibody Response

Nakano

Shimamura

Saito

1971

Japanese Journal of Microbiology

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Adjuvant action of lipopolysaccharide (LPS) extracted from Salmonella typhimurium LT2 on anti-sheep red blood cell (sRBC) antibody response in mouse spleen was studied at the cellular level by the technique of localized hemolysis in agar. Injection of LPS caused significant increase in direct 19S and indirect 7S plaque-forming cells (PFC) in mouse spleen after the primary antigenic stimulation, while the adjuvant effect of LPS could hardly be assessed in the secondary PFC response. The adjuvant effect on the primary PFC response was most conspicuous when LPS was injected at the same time as the sRBC, and no effect could be observed when LPS was injected 1 or 5 hr before PFC determination. The mice who had received LPS with the primary sRBC stimulation showed much higher PEG level after the secondary sRBC stimulation, indicating the increase in the memory cells. Cell population consisting of lymphoid cells and macrophages was separated into 'adherent' (macrophage-rich) and 'non-adherent' (lymphoid cell-rich) fractions by incubating in petri dishes, and effect of LPS on each fraction was examined. Transfer of non-adherent lymphoid cells treated with LPS in vitro and simultaneous sRBC injection resulted in a significant increase of PFC in the spleen of the syngeneic recipient mice, but transfer of LPS-treated macrophages did not show any effect, suggesting that LPS acts on lymphocytes but not on macrophages. This idea appeared also to be supported by the results that LPS exerted antagonistic effect on the immune suppression caused by heterologous anti-lymphocyte serum, anti-sRBC serum and cortisone which are currently supposed to suppress the antigen reactive cells.

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Section: Discussionmentioning

confidence: 80%

Cellular Mechanisms of Adjuvant Action of Bacterial Lipopolysaccharide in Anti‐Sheep Red Blood Cell Antibody Response

Nakano

Shimamura

Saito

1971

Japanese Journal of Microbiology

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“…4 and 5, since erythrocytes sensitized with moderate amounts of carbohydrate antigen yielded comparable results. A PFC response was first detectable at day 2, reached a peak at day 3 Effect of enzymatic treatment of the ribosomes on their PFC-enhancing activity in mice, 3 days after injection. Mean of two experiments, each involving three mice per group.…”

Section: Fig 2 Immunodiffusion Patterns Of Rabbit Antiserummentioning

confidence: 99%

Response of Mice to Injection of Ribosomal Fraction from Group B Neisseria meningitidis

Thomas

Weiss

1972

Infect Immun

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Ribosomes of strain NOR-7 of group B Neisseria meningitidis were isolated by a procedure that included treatment of the cells with sodium dodecyl sulfate, disruption in a French pressure cell, and differential centrifugation. These preparations consisted of 66% ribonucleic acid and 24% protein and sedimented as a single component with a constant of approximately 66 S. When used in immunodiffusion tests with homologous rabbit antiserum, untreated ribosomes formed two precipitin lines, when treated with ribonuclease three lines, and when Pronase-digested only one distinct line. Qualitatively indistinguishable reactions were obtained with the same antiserum and ribosomes from group A meningococci, but no precipitation occurred with those of Escherichia coli. When injected into mice, group B ribosomes elicited an increase in the number of antibody-producing spleen cells demonstrable by the hemolytic plaque technique using unsensitized sheep erythrocytes. Sensitization of the erythrocytes with increasing amounts of supernatant fluid of meningococcal cultures progressively reduced the number of demonstrable plaque-forming cells. Neuraminidase treatment of the erythrocytes increased immune hemolysis, whereas Pronase digestion reduced it. Injected mice were protected against homologous and heterologous meningococcal challenge. Both hemolysis and protection-inducing activities of the ribosomes were unimpaired by ribonuclease, but were reduced by Pronase. It is concluded that the immunological response elicited by the meningococcal ribosomes does not involve the group-specific carbohydrate antigen. The immunological mechanism by which the mice are protected against meningococcal challenge remains unknown.

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Nucleoside und Nucleotide als mögliche Therapeutika

Shen

1970

Angewandte Chemie

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Biodynamic effects of oligonucleotides.

Cited by 17 publications

References 34 publications

Cellular Mechanisms of Adjuvant Action of Bacterial Lipopolysaccharide in Anti‐Sheep Red Blood Cell Antibody Response

Cellular Mechanisms of Adjuvant Action of Bacterial Lipopolysaccharide in Anti‐Sheep Red Blood Cell Antibody Response

Response of Mice to Injection of Ribosomal Fraction from Group B Neisseria meningitidis

Nucleoside und Nucleotide als mögliche Therapeutika

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