Steviol glycosides and polyphenols extraction from <i>Stevia rebaudiana</i> Bertoni leaves using maceration, microwave-, and ultrasound-assisted techniques

Yılmaz, Fatih; Görgüç, Ahmet; Uygun, Özge; Bircan, Cavit

doi:10.1080/01496395.2020.1743311

Cited by 42 publications

(29 citation statements)

References 51 publications

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“…Metabolic disorders such as type-II diabetes and obesity, associated with an excessive sugar consumption, are becoming more prevalent and stevia is the perfect sugar substitute in foods and drinks, given that its leaves contain non-calorie high-intensive sweetener compounds, namely steviol glycosides (SVglys) [9][10][11]. Its leaves also contain a complex mixture of triterpenoids, sterols, essential oils, phenols, and flavonoids, with functional and health-promoting properties [12][13][14][15][16][17]. Thanks to its positive agronomic and phytochemical characteristics, stevia could thus offer new solutions in terms of cropping system diversification and marketing opportunities.…”

Section: Introductionmentioning

confidence: 99%

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

2021

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Stevia rebaudiana (Bertoni) is a promising medicinal and aromatic plant for Mediterranean agroecosystems given its positive agronomic attributes and interesting quality features. It has both food and pharmaceutical applications, since its leaves contain sweet-tasting steviol glycosides (SVglys) and bioactive compounds, such as phenolics, flavonoids, and vitamins. We evaluated the agronomic and qualitative performances of nine stevia genotypes cultivated, in open field conditions, for two consecutive years under the Mediterranean climate of central Italy. Growth, biomass production, and accumulation of bioactive compounds (SVglys, polyphenols, and their related antioxidant activities) were evaluated, considering the effect of harvest time and crop age (first and second year of cultivation). The results showed high variability among genotypes in terms of both morpho-productive and phytochemical characteristics. In general, greater leaf dry yields, polyphenol accumulation, and antioxidant activities were found in the second year of cultivation, harvesting the plants in full vegetative growth. On the other hand, total SVglys leaf content reached the highest values in the first year when plants were at the beginning of the reproductive phase. On the other hand, although the SVglys profile (Rubusoside, Dulcoside A, Stevioside, Rebaudioside A, C, D, E, and M) remained stable over harvest times, it differed significantly depending on the crop age and genotype. Our findings provide useful information on the influence of crop age and harvest time in defining quanti-qualitative traits in stevia, with PL, SL, BR5, and SW30 being the best performing genotypes and thus suitable for breeding programs. Our study highlighted that stevia, in the tested environment, represents a promising semi-perennial crop which offers new solutions in terms of cropping system diversification and marketing opportunities.

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Section: Introductionmentioning

confidence: 99%

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

2021

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“…Procedure time (1–25 min), stirring (250 rpm), and temperature (20–50 °C) are used. UAE is carried out in an ultrasound bath or probe and plant tissues are destroyed through ultrasonic waves, as they cause mechanical vibrations that lead to expansion and compression cycles during movement through the extraction medium and provoke the rise of temperature and negative pressure [ 19 , 20 ]. These mechanical and thermal effects cause the degradation of cell walls, the release of cell contents, greater penetration of solvent into plant material, the increment of mass transfer, and thus, the increase of PPs yield [ 21 ].…”

Section: Extraction Of Ppsmentioning

confidence: 99%

“…Besides, RSLDE uses the Naviglio extractor generation in which a suitable solvent is used under the pressure gradient between the outside and the inside of a solid matrix containing extractable material, followed by a sudden restoration of the initial equilibrium conditions, induces forced extraction [ 23 ]. Recently, Yilmaz et al [ 19 ] reported a study for the extraction of PPs using maceration, MAE, and UAE methods from Stevia rebaudiana Bertoni. In that study, MAE and UAE required less processing time and showed a higher yield as compared to maceration [ 19 ].…”

Section: Extraction Of Ppsmentioning

confidence: 99%

“…Recently, Yilmaz et al [ 19 ] reported a study for the extraction of PPs using maceration, MAE, and UAE methods from Stevia rebaudiana Bertoni. In that study, MAE and UAE required less processing time and showed a higher yield as compared to maceration [ 19 ]. Galan et al [ 24 ] carried out a comparison study between MAE and conventional health-assisted extraction; they stated that MAE possessed higher yield and antioxidant activity for PPs.…”

Section: Extraction Of Ppsmentioning

confidence: 99%

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Curcumin, Quercetin, Catechins and Metabolic Diseases: The Role of Gut Microbiota

et al. 2021

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Polyphenols (PPs) are the naturally occurring bioactive components in fruits and vegetables, and they are the most abundant antioxidant in the human diet. Studies are suggesting that ingestion of PPs might be helpful to ameliorate metabolic syndromes that may contribute in the prevention of several chronic disorders like diabetes, obesity, hypertension, and colon cancer. PPs have structural diversity which impacts their bioavailability as they accumulate in the large intestine and are extensively metabolized through gut microbiota (GM). Intestinal microbiota transforms PPs into their metabolites to make them bioactive. Interestingly, not only GM act on PPs to metabolize them but PPs also modulate the composition of GM. Thus, change in GM from pathogenic to beneficial ones may be helpful to ameliorate gut health and associated diseases. However, to overcome the low bioavailability of PPs, various approaches have been developed to improve their solubility and transportation through the gut. In this review, we present evidence supporting the structural changes that occur after metabolic reactions in PPs (curcumin, quercetin, and catechins) and their effect on GM composition that leads to improving overall gut health and helping to ameliorate metabolic disorders.

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“…bines different techniques such as maceration, microwave, vacuum, and ultrasound to achieve better results of non-oxidized bioactive compounds evaluated in different vegetables such as stevia leaves, rose leaves, Alpinia oxyphylla Miq leaves, and fruits of sohiong (Dash et al, 2021;Hasan et al, 2019;Kasara et al, 2020a;Niu et al, 2020;Yılmaz et al, 2020); in these studies, they found that selectivity preservation of antioxidant compounds present in vegetables is achieved as the microwave is used as part of the drying or extraction process (Dash et al, 2021;Kasara et al, 2020a). One of the identified variables in the drying process for preservation purposes was the microwave power; this power, in some studies, shows that preservation results of antioxidant compounds are obtained in values around 400 W-average power from 100 to 900 W (Kasara et al, 2020a).…”

Section: The Preservation Of Antioxidant Compounds In Vegetables Com-mentioning

confidence: 99%

Microwave‐assisted forced convection drying effect on bioactive compounds of the Canadian blueberry leaves (Vaccinium corymbosum)

Borda-Yepes

Janna

Granados

et al. 2021

J. Food Process. Preserv.

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Canadian blueberry leaves (Vaccinium corymbosum) are a potential source of polyphenols (catechins) and antioxidants (FRAP and PT). In this study, the technique of drying by microwave-assisted forced convection was carried out to find the optimal conditions which would allow obtaining the highest polyphenol content under control parameters such as microwave power (100, 300, and 400 W), hot air velocity (0.5 and 2.0 m/s), leaf size (whole and fractional), and temperatures (50, 60, and 70°C). It was found that 30 W/g microwave powers and a 60°C temperature for the process in whole leaves were the optimal conditions that allowed obtaining the highest content of polyphenols (>10 mg/gdb (+) catechin) and antioxidant capacities (100-150 mg GAE/gdb), more than in fractional leaves. It was observed that different hot air velocities did not affect the concentration of the polyphenol's contents; this was verified using the factor mixture analysis technique. Practical applicationsThe MWFC process allows the preservation of antioxidants in Canadian blueberry leaves (Vaccinium corymbosum). These leaves have high antioxidant capacity for nutraceutical and pharmaceutical uses. This leaf, with high polyphenol content, can be used to obtain extracts in infusions and human food through the mixture of this leaf with multiple powdered products traditionally consumed or food supplements. This research presents the optimal conditions for the preservation of antioxidant compounds in dried Canadian blueberry leaves. Highlights• Polyphenolic compounds (catechins) are retained under controlled conditions of microwave drying assisted by forced convection in Canadian blueberry leaves.

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Steviol glycosides and polyphenols extraction from Stevia rebaudiana Bertoni leaves using maceration, microwave-, and ultrasound-assisted techniques

Cited by 42 publications

References 51 publications

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

Stevia rebaudiana (Bertoni) as a Multifunctional and Sustainable Crop for the Mediterranean Climate

Curcumin, Quercetin, Catechins and Metabolic Diseases: The Role of Gut Microbiota

Microwave‐assisted forced convection drying effect on bioactive compounds of the Canadian blueberry leaves (Vaccinium corymbosum)

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