Steroid hormone receptors ERα and PR characterised by immunohistochemistry in the mare adrenal gland

Alm, Ylva Hedberg; Sukjumlong, Sayamon; Kindahl, H.; Dalin, Anne-Marie

doi:10.1186/1751-0147-51-31

Cited by 8 publications

(2 citation statements)

References 32 publications

(50 reference statements)

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“…Protein localization of ER-␣, ER-␤, PR, EP2 and EP4 in uterine tissue was investigated by immunohistochemistry (IHC) using antibodies previously validated for the horse (Abd-Elnaeim et al, 2009;Alm et al, 2009;Ball et al, 2013;Parlevliet et al, 2006;Pearl et al, 2011). Endometrium and myometrium were fixed in 10% formalin for 24 h. After the 24 h fixation period, samples were dehydrated and embedded in paraffin.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Expression of receptors for ovarian steroids and prostaglandin E2 in the endometrium and myometrium of mares during estrus, diestrus and early pregnancy

Silva

Scoggin

Canisso

et al. 2014

Animal Reproduction Science

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Section: Immunohistochemistrymentioning

confidence: 99%

Expression of receptors for ovarian steroids and prostaglandin E2 in the endometrium and myometrium of mares during estrus, diestrus and early pregnancy

Silva

Scoggin

Canisso

et al. 2014

Animal Reproduction Science

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“…Likewise, the basal CORT pattern differed between intact and ovariectomized mares, suggesting that E2 may affect the adrenal function. Indeed, Alm et al [ 44 ] showed E2 receptors (ER) in cortex and adrenal medulla in ovariectomized mares, showing that the ovulatory dynamics could be the result in the same way of ACTH and CORT effects. Hence, it is possible to suggest the interaction among pre-ovulatory follicle, hypophysis, and adrenal synthesis during the ovulatory period.…”

Section: Discussionmentioning

confidence: 99%

Endocrine and Electrolyte Balances during Periovulatory Period in Cycling Mares

Satué

Fazio

Muñoz

et al. 2021

Animals

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In cycling females, the periovulatory period is characterized by stimulation of the hypothalamic pituitary adrenal (HPA) axis. The aim of present study was to analyze the pattern and interrelationships among adrenocorticotropic hormone (ACTH), cortisol (CORT), aldosterone (ALD) and electrolytes (sodium—Na+, potassium—K+ and chloride—Cl−) during periovulatory period in cycling mares. Venous blood samples were obtained daily from a total of 23 Purebred Spanish broodmares, aged 7.09 ± 2.5 years, from day −5 to day +5 of estrous cycle, considering day 0, the day of ovulation. Plasma ACTH was measured by a fluorescent immunoassay kit, serum CORT and ALD by means of a competitive ELISA immunoassay, and plasma Na+, K+ and Cl− were quantified by an analyzer with selective electrodes for the three ions. ACTH showed higher concentrations at day 0 compared to days −5 to −1 and +1 to +3 (p < 0.05). CORT showed higher concentrations at day 0 compared to days −5 to −2 and +1 to +5 (p < 0.05). ALD showed higher concentrations at day 0 compared to days −5 to −2 (p < 0.05) and +2 (p < 0.05). Na+ and Cl− showed higher concentrations at day 0, compared to day −5 and +5. K+ showed lower concentrations at day 0 compared to day +1 (p < 0.05). The significant correlations obtained between ACTH and CORT (r = 0.20) and between ACTH and ALD (r = 0.32) suggest that although ACTH may have an effect both on CORT and ALD, there are other very important determinants that could be considered. Hence, it is possible to presume that the pituitary adrenocortical response and ALD may be involved in the ovulatory mechanisms without a direct relation with electrolyte pattern.

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Hemicastration induced stimulation of pineal and adrenocortical karyomorphology and cell proliferation can be reversed by steroid hormone and antihormone modulations in mice (Mus musculus)

et al. 2010

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Hemicastration has often been used to investigate the effect of in vivo modulation of gonadotrophin and steroids on peripheral endocrine organs. In the present study the effect of hemicastration was studied on pinealadrenal karyomorphology and percentage of cell proliferation activity and it was further examined whether such relationship was influenced through administration of steroid hormone and antihormones. In the present study the post pubertal male mice were hemicastrated and after thirty days of post hemicastration, the mice groups were administrated separately with steroid hormones estradiol at a dose of 5 µg/100 g b.w., testosterone of a dose of 100 µg/100 g b.w. and antihormones, tamoxifen at a dose of 500 µg/100 g b.w. and flutamide 2 mg/100 g b.w. daily for ten consecutive days. Our data revealed that both the pineal and adrenal gland nuclear size and cell proliferation were significantly increased in thirty days post hemicastrated male mice. It was further observed that the steroid hormones, estradiol and testosterone and antihormones administered for ten consecutive days significantly reversed the pineal adrenal hyperactivity and hyperplasia induced by hemicastration. Taken together our current experiments showed that both the pineal and adrenal glands stimulated following hemicastration can K O LK ATA RESEARCH ARTICLEadversely respond to input of steroid hormones and antihormones showing reversal of hemicastration induced stimulation in these post pubertal male mice.

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Steroid hormone receptors ERα and PR characterised by immunohistochemistry in the mare adrenal gland

Abstract: Background: Sex steroid hormone receptors have been identified in the adrenal gland of rat, sheep and rhesus monkey, indicating a direct effect of sex steroids on adrenal gland function.

Cited by 8 publications

References 32 publications

Expression of receptors for ovarian steroids and prostaglandin E2 in the endometrium and myometrium of mares during estrus, diestrus and early pregnancy

Expression of receptors for ovarian steroids and prostaglandin E2 in the endometrium and myometrium of mares during estrus, diestrus and early pregnancy

Endocrine and Electrolyte Balances during Periovulatory Period in Cycling Mares

Hemicastration induced stimulation of pineal and adrenocortical karyomorphology and cell proliferation can be reversed by steroid hormone and antihormone modulations in mice (Mus musculus)

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