Hemicastration induced stimulation of pineal and adrenocortical karyomorphology and cell proliferation can be reversed by steroid hormone and antihormone modulations in mice (Mus musculus)

Abstract

Hemicastration has often been used to investigate the effect of in vivo modulation of gonadotrophin and steroids on peripheral endocrine organs. In the present study the effect of hemicastration was studied on pinealadrenal karyomorphology and percentage of cell proliferation activity and it was further examined whether such relationship was influenced through administration of steroid hormone and antihormones. In the present study the post pubertal male mice were hemicastrated and after thirty days of post hemicastration, the mice groups were administrated separately with steroid hormones estradiol at a dose of 5 µg/100 g b.w., testosterone of a dose of 100 µg/100 g b.w. and antihormones, tamoxifen at a dose of 500 µg/100 g b.w. and flutamide 2 mg/100 g b.w. daily for ten consecutive days. Our data revealed that both the pineal and adrenal gland nuclear size and cell proliferation were significantly increased in thirty days post hemicastrated male mice. It was further observed that the steroid hormones, estradiol and testosterone and antihormones administered for ten consecutive days significantly reversed the pineal adrenal hyperactivity and hyperplasia induced by hemicastration. Taken together our current experiments showed that both the pineal and adrenal glands stimulated following hemicastration can K O LK ATA RESEARCH ARTICLEadversely respond to input of steroid hormones and antihormones showing reversal of hemicastration induced stimulation in these post pubertal male mice.