Expression of receptors for ovarian steroids and prostaglandin E2 in the endometrium and myometrium of mares during estrus, diestrus and early pregnancy

Silva, Elisa Sant'Anna Monteiro da; Scoggin, Kirsten E.; Canisso, Igor F.; Troedsson, Mats H.T.; Squires, E.L.; Ball, Barry A.

doi:10.1016/j.anireprosci.2014.11.001

Cited by 34 publications

(42 citation statements)

References 64 publications

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“…ERα was found to be differentially expressed along the different stages of the oestrous cycle in pigs (Sukjumlong et al, ), horses (Silva et al, ) and cows (Kimmins & Maclaren, ). In this study, we detected differences along the oestrous cycle along with differences between abundance of hormone receptors in sheep myometrium and endometrium.…”

Section: Discussionmentioning

confidence: 99%

“…ERα was found to be differentially expressed along the different stages of the oestrous cycle in pigs (Sukjumlong et al, 2009), horses (Silva et al, 2014) and cows (Kimmins & Maclaren, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Progesterone is secreted to maintain uterine growth and pregnancy (Chen, Cai, Wang, & Xu, 2015). Progesterone nuclear receptor expression has been documented in the uterus of cattle (Kimmins & Maclaren, 2001), cow (Robinson, Mann, Lamming, & Wathes, 2001), horse (Silva et al, 2014) and human (Amso et al, 1994), indicating that PGR is essential for uterus functioning.…”

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confidence: 99%

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Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

Duan

Xiao

et al. 2019

Reprod Domestic Animals

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Oestrogen, androgen and progesterone are involved in the regulation of uterine physiological functions, with the participation of the following proteins: oestrogen receptor (ER), androgen receptor (AR) and progesterone nuclear receptor (PGR). In this study, we used immunohistochemistry to detect the localization of ERα, ERβ, AR and PGR in sheep uterus. Additionally, we used real‐time polymerase chain reaction (RT‐qPCR) and Western blot technique to analyse their expression profiles at different stages of sheep oestrous cycle in the endometrium and myometrium. Immunohistochemical analysis showed that ERα, ERβ, AR and PGR were present in sheep uterus in oestrus, mainly in the uterine luminal epithelium, stroma, gland and myometrium. Real‐time polymerase chain reaction results showed that in the endometrium, ERα expression level was highest in oestrus. ERβ and PGR, instead, were highly expressed in pro‐oestrus. In the myometrium, ERα was highly expressed in both oestrus and pro‐oestrus, and ERβ was highly expressed in oestrus and dioestrus. Progesterone nuclear receptor expression was highest in oestrus, followed by metoestrus. In the endometrium, both receptors ERα and ERβ were abundant in pro‐oestrus, while the maximum AR protein content was found in oestrus. At this stage of the oestrous cycle, PGR protein concentration in the myometrium was significantly lower than those observed in other stages. These results suggest that these receptors are important for sheep reproductive function, as their expression at mRNA and protein levels exhibits particular time‐ and tissue‐specific profiles along the oestrous cycle.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

Duan

Xiao

et al. 2019

Reprod Domestic Animals

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“…The main ovarian steroids that rule reproductive physiological events in the mare are estrogens (E2) synthetized by ovarian follicles in the follicular phase, and progesterone (P4) produced by the corpus luteum (CL) in the luteal phase. In fact, in the mare endometrium, during estrus, high levels of ovarian steroid receptors, such as ER , ER and PR mRNA and protein have been detected in luminal and glandular epithelia, as well as in stromal cells (Watson et al 1992, Gebhardt et al 2012, Silva et al 2014. In addition, a positive correlation between mRNA expression of ER and ER has been reported in mare endometrium (Honnens et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…In the luteal phase, when circulating P4 levels are high, endometrium expression of ER and PR are inhibited. In mare endometrium, E2 up-regulates ER and ER nuclear receptors, and also P4 receptors (PR) (Silva et al 2014). When ovarian steroids reach the equine endometrium, they modulate cell proliferation, angiogenesis and prostaglandin (PG) secretion (Galvão et al 2013, Szóstek et al 2014.…”

Section: Introductionmentioning

confidence: 99%

Dual effect of hormones on mare reproductive physiology and dysfunction

Rebordão¹,

Amaral²,

Galvão³

et al. 2016

PHK

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Summary: In the last decades, classical and molecular biology approaches have greatly contributed for building up sound knowledge on the mechanisms regulating reproductive processes in the mare. Different perspectives on the role of hormones should be addressed and thus considered by both researchers and clinicians. While oxytocin is largely used as a post-insemination method to improve fertility or prevent endometritis, it may also be considered to prolong the luteal phase. Prostaglandin E2 (PGE2), known to sustain luteostasis, has also been related to inflammatory processes in sub-clinical endometritis in mares. In contrast, as other ecbolic agents, PGF2a besides being luteolytic and currently used for estrous cycle shortening and endometritis control, might also play a role in fibrogenesis in mare endometrium. Hormones, like oxytocin, ovarian steroids, prostaglandins (PG), as well as enzyme profiles involved in endocrine pathways may act in a dissimilar fashion, depending on the estrous cycle phase and on the physiologic or pathologic condition.Keywords: mare / reproduction / oxytocin / prostaglandin / endometrium / fibrosis / ovarian steroids Doppeleffekt von Hormonen auf die Reproduktionsphysiologie und -störung bei der StuteIn den letzten Jahrzehnten haben die klassischen und molekularbiologische Methoden in hohen Maße für die Entwicklung fundierten Wissens zu den Regelmechanismen reproduktiver Prozesse bei der Stute beigetragen. Es sollten die unterschiedliche Sichtweisen auf die Rolle der Hormone berücksichtigt werden, sowohl von Forschern als auch Klinikern. Während Oxytocin überwiegend als Verfahren nach der Insemination verwendet wird, um die Fruchtbarkeit zu verbessern oder eine Endometritis zu verhindern, kann es auch eingesetzt werden, um die Gelbkörperphase zu verlängern. Prostaglandin E2 (PGE2) ist bekannt, die Luteostase zu erhalten, wird aber auch mit entzündlichen Prozessen bei der equinen subklinische Endometritis in Verbindung gebracht. Im Gegensatz dazu könnte PGF2a, wie andere ekbolische Mittel, abgesehen davon, dass es luteolytisch wirkt und derzeit benutzt wird, den Ovarialzyklus zu verkürzen und eine Endometritis zu kontrollieren, auch eine Rolle bei der Fibrogenese im equinen Endometrium spielen. Hormone wie Oxytocin, Ovarialsteroide, Prostaglandine (PG), als auch Enzymprofile, die an endokrinen Regelkreisen beteiligt sind, können in einer unterschiedlichen Art und Weise wirken, abhängig von der Zyklusphase und dem physiologischen oder pathologischen Zustand.

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Sex-steroid receptors, prostaglandin E2 receptors, and cyclooxygenase in the equine cervix during estrus, diestrus and pregnancy: Gene expression and cellular localization

Fernandes

Loux

Scoggin

et al. 2017

Animal Reproduction Science

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Expression of receptors for ovarian steroids and prostaglandin E2 in the endometrium and myometrium of mares during estrus, diestrus and early pregnancy

Cited by 34 publications

References 64 publications

Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

Dual effect of hormones on mare reproductive physiology and dysfunction

Sex-steroid receptors, prostaglandin E2 receptors, and cyclooxygenase in the equine cervix during estrus, diestrus and pregnancy: Gene expression and cellular localization

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