Steroid Biosynthesis 'In Vitro' by Granulosa-Theca Cell Tumour Tissue

Griffiths, K.; Grant, J. K.; Symington, T.

doi:10.1677/joe.0.0300247

Cited by 19 publications

(3 citation statements)

References 17 publications

(25 reference statements)

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“…Both steroids have been isolated after incubation of progesterone with human ovarian tissue (Griffiths et al 1964) and with homogenate preparation of human myo¬ metrium (Junkermann et al 1977, in press). The former compound was also isolated as metabolite of [4-14C] progesterone in the ovine blood (Seamark et al 1970).…”

Section: Discussionmentioning

confidence: 99%

Metabolism of Progesterone in Uterine Tissue of Non-Pregnant Rats in Vitro

Lisboa

Holtermann

1976

Acta Endocrinologica

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The metabolism of labelled progesterone was studied in vitro in uterine tissue of non-pregnant rats with particular emphasis on the influence of substrate concentration. Neither a qualitative nor quantitative difference was found for a steroid tissue ratio between 15 \m=x\10\m=-\6and 4.2 \m=x\10\m=-\9 to 1 g (substrate amounts between 57.73 and 0.02 nmol); with both concentrations 42 to 44 per cent of progesterone was metabolized to about 35 per cent monohydroxymonoketonic steroids and 4\p=n-\6 per cent dihydroxylated C21O2-compounds.In both sets of incubations we have isolated and identified the following steroids: 3\g=a\-hydroxy-5\g=a\-pregnan-20-one,3\g=b\-hydroxy-4-pregnen-20-one, 3\g=a\-hydroxy-4-pregnen-20-one,20\g=a\-hydroxy-4-pregnen-3-one,5\g=a\-pregnane-3\g=a\,20\g=a\-dioland 4-pregnene-3\g=a\,20\g=a\-diol. The most abundant metabolite formed in these incubations was 3\g=a\-hydroxy-4-pregnen-20-one \ x =r eq-\ which corresponds to about 30 per cent of the total activity recovered. It is the first time that the presence of 20\g=a\-hydroxysteroid-oxidoreductase activity is definitely established in this type of tissue. The identification of three allylic alcohols as progesterone metabolites in the rat uterus confirms that \g=D\4-3-hydroxysteroids are important intermediates in the in vitro uterine metabolism of steroids.

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Section: Discussionmentioning

confidence: 99%

Metabolism of Progesterone in Uterine Tissue of Non-Pregnant Rats in Vitro

Lisboa

Holtermann

1976

Acta Endocrinologica

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“…Earlier studies (Griffiths, Grant & Symington, 1964;Griffiths et al 19666) suggested that granulosa cell tumour tissue does not utilize progesterone for oestrogen formation. Furthermore, incubation with…”

Section: Discussionmentioning

confidence: 99%

A COMPARISON OF THE METABOLISM IN VITRO OF [7α-3h] DEHYDROEPIANDROSTERONE SULPHATE AND [4-14c] PREGNENOLONE BY TISSUE FROM a HILUS CELL TUMOUR OF THE OVARY

Fahmy¹,

Griffiths²,

Turnbull³

et al. 1968

Journal of Endocrinology

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Tissue from a hilus cell tumour of an ovary removed from a 58-yr.-old virilized woman was incubated simultaneously with [7\g=a\-3H]dehydroepiandrosterone sulphate (DHA sulphate) and [4-14C]pregnenolone. The tissue was chopped and incubated in Krebs-Ringer bicarbonate glucose medium without the addition of cofactors. Both steroids were efficiently metabolized by the tissue; 78\m=.\5and 1\m=.\2 % of the pregnenolone was converted to androstenedione and testosterone respectively, whereas 34\m=.\6 % of the incubated DHA sulphate was isolated in the carrier DHA, 41\m=.\4% in the androstenedione and 0\m=.\5% in the testosterone.

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“…Measurements of steroid excretion before and after surgery have shown that a large proportion of urinary oestrogens may originate from ovarian tumours (Brown et af., 195Y;Brown & Beischer, 1972;Targett, 1974). In vitro studies have demonstrated that tissue obtained from oestrogen producing ovarian tumours has the ability t o synthesize both oestrone and oestradiol (Griffiths et al, 1964;Besch et al, 1966) although in v i~~ secretion o f these steroids by such tumours has not yet been demonstrated.…”

mentioning

confidence: 99%

Plasma Hormone Levels and Oestrogen Production in a Postmenopausal Woman With Endometrial Carcinoma and an Ovarian Thecoma

Reed

Hutton

Beard

et al. 1979

Clinical Endocrinology

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SUMMARY Plasma hormone concentrations, the production rate of oestrone from androstenedione, and the total production rate of oestrone have been measured in a 57‐year‐old woman with an ovarian thecoma and endometrial carcinoma before and 2 months after hysterectomy and bilateral ovariectomy. The plasma concentration of oestradiol (184 pmol/l), but not oestrone (144 pmol/l), was elevated pre‐operatively and FSH secretion was suppressed (115.0 μg/l). Post‐operatively the plasma concentration of oestradiol was significantly reduced (80 pmol/l) and the concentration of FSH (571 μg/l) had increased to a postmenopausal level. The concentration of oestradiol in samples of plasma obtained simultaneously at operation from the ovarian vein of the ovary containing the thecoma (3.38 nmol/l) was fifteen times greater than in the periphery (228.0 pmol/l), and this confirmed that oestradiol was secreted by the thecoma. The total production rates of oestrone pre‐and post‐operatively were 101.3 nmo1/24 h and 29.2 nmo1/24 h respectively and the production rates of oestrone from androstenedione were 41.4 nmo1/24 h and 32.9 nmo1/24 h. Thus, postaperatively, all the oestrone was produced from circulating androstenedione, whereas pre‐operatively only 40% of the oestrone was produced by the extraglandular conversion of androstenedione; the remaining 60% presumably originated from the peripheral conversion of oestradiol secreted by the thecoma. It is concluded that the oestradiol secreted by the thecoma was probably the major factor which caused the development of endometrial carcinoma in this subject.

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Steroid Biosynthesis 'In Vitro' by Granulosa-Theca Cell Tumour Tissue

Cited by 19 publications

References 17 publications

Metabolism of Progesterone in Uterine Tissue of Non-Pregnant Rats in Vitro

Metabolism of Progesterone in Uterine Tissue of Non-Pregnant Rats in Vitro

A COMPARISON OF THE METABOLISM IN VITRO OF [7α-3h] DEHYDROEPIANDROSTERONE SULPHATE AND [4-14c] PREGNENOLONE BY TISSUE FROM a HILUS CELL TUMOUR OF THE OVARY

Plasma Hormone Levels and Oestrogen Production in a Postmenopausal Woman With Endometrial Carcinoma and an Ovarian Thecoma

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