The metabolism of labelled progesterone was studied in vitro in uterine tissue of non-pregnant rats with particular emphasis on the influence of substrate concentration. Neither a qualitative nor quantitative difference was found for a steroid tissue ratio between 15 \m=x\10\m=-\6and 4.2 \m=x\10\m=-\9 to 1 g (substrate amounts between 57.73 and 0.02 nmol); with both concentrations 42 to 44 per cent of progesterone was metabolized to about 35 per cent monohydroxymonoketonic steroids and 4\p=n-\6 per cent dihydroxylated C21O2-compounds.In both sets of incubations we have isolated and identified the following steroids: 3\g=a\-hydroxy-5\g=a\-pregnan-20-one,3\g=b\-hydroxy-4-pregnen-20-one, 3\g=a\-hydroxy-4-pregnen-20-one,20\g=a\-hydroxy-4-pregnen-3-one,5\g=a\-pregnane-3\g=a\,20\g=a\-dioland 4-pregnene-3\g=a\,20\g=a\-diol. The most abundant metabolite formed in these incubations was 3\g=a\-hydroxy-4-pregnen-20-one \ x =r eq-\ which corresponds to about 30 per cent of the total activity recovered. It is the first time that the presence of 20\g=a\-hydroxysteroid-oxidoreductase activity is definitely established in this type of tissue. The identification of three allylic alcohols as progesterone metabolites in the rat uterus confirms that \g=D\4-3-hydroxysteroids are important intermediates in the in vitro uterine metabolism of steroids.
Kan psykose forebygges? 950Studier antyder at kognitiv atferdsterapi kan forhindre eller utsette psykose hos høyrisikopersoner.
In vitro experiments carried out with uterus preparations of ovariectomized adult rats indicate the presence in this tissue of a 20\g=b\-hydroxysteroid-oxidoreductase which catalyzes the conversion of 20\g=b\-hydroxy-4\x=req-\ pregnen-3-one to progesterone.Since a hepatic 20\g=b\-hydroxysteroid-oxidoreductase is absent in adult female rats, the myometrial enzyme can be responsible for the biological activity of 20\g=b\-hydroxy-4-pregnen-3-one in these animals.Besides progesterone five metabolites were isolated and identified after incubation of [4-14C]20\g=b\-hydroxy-4-pregnen-3-one with uterine tissue: 20\g=b\-hydroxy-5\g=a\-pregnan-3-one, 20\g=b\-hydroxy-5\g=b\-pregnan-3-one, 5\g=a\-pregnane-3\g=a\,20\g=b\-diol,4-pregnene-3\g=a\,20\g=b\-diol and 4-pregnene-3\g=b\,20\g=b\-diol. The conversion of 20\g=b\-hydroxy-4-pregnen-3-one to progesterone permits This work was supported by the Deutsche Forschungsgemeinschaft, Sonderforschungs¬ bereich -Endokrinologie 34. This paper is abstracted from a thesis submitted by M. Holtermann in partial fulfilment of the requirements for the degree of Doctor of Medicine in the Medical School of the University of Hamburg. Trivial names and abbreviations used in the text Progesterone = 4-pregnene-3,20-dione, 17a-hydroxyprogesterone = 17a-hydroxy-4-pregnene-3,20-dione, cortisol = 11/?,17, 21-trihydroxy-4-pregnene-3,20-dione, cortisone = 17,21-dihydroxy-4-pregnene-3,11,20-trione, tetrahydrocortisone = 3a,17,21-trihydroxy-5/?-pregnane-ll,20-dione, /i-cortolone = 3a, 17a,20/5,21-tetrahydroxy-5/?-pregnan-11-one, tK = relative retention time (to chole¬ stane). RGC = radio gas chromatography, GCC = gel column chromatography. TLC = thin-layer chromatography, TMSi = trimethylsilylether, MoTMSi = methoxime-trimethylsilylether.
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