Stereoselective determination of drugs and metabolites in body fluids, tissues and microsomal preparations by capillary electrophoresis (2000–2010)

Pre-Concentration Approaches Combined with Capillary Electrophoresis in Bioanalysis of Chiral Cardiovascular Drugs

“…2,5 In better words, almost 1 in 4 of marketed drugs is in racemic form rather than their enantiomers. 6 The fact that 9 of top 10 drugs have chiral active ingredients, may emphasize the important aspect of chiral drugs in pharmaceutical investigations. In general, administration of more active isomer of racemic drugs causes to following advantages: diminishing probable side effects due to the antipode (counterpart) isomer, decrease in producing of unwanted isomer, halved dosage for a patient and new commercial opportunities for racemic switching.…”

Chiral analysis of amphetamines, methadone and metabolites in biological samples by electrodriven methods

“…The electrophoretic conditions developed by Frost et al [61] and the sample pre-treatment procedure developed by Lanz and Thormann [62] were subsequently used to assess the stereoselectivity of MD metabolism by human microsomes (Fig. 10) and by different CYP enzymes [64,65].…”

Capillary and Microchip Electrophoresis

“…Of particular interest are stereoselective assays such as CZE, EKC, and CEC separations in presence of one or a mixture of chiral selectors that resolve enantiomers and other stereoisomers [129][130][131][132]. Such assays are well suited to investigate stereoselective drug metabolism in biofluids, tissues, and microsomal preparations [133,134]. With highresolution CZE and EKC assays, pharmaceuticals and many of their phase I and II metabolites can be separated and analyzed in a single run.…”

“…With highresolution CZE and EKC assays, pharmaceuticals and many of their phase I and II metabolites can be separated and analyzed in a single run. Separation media can be adjusted to be applicable to hydrophilic and hydrophobic drugs and metabolites [123][124][125][126][127][128][129][130][131][132][133][134]. A CZE assay for therapeutic drug monitoring of lamotrigine has been routinely used since the 2000s [105].…”