Capillary and Microchip Electrophoresis

Thormann, Wolfgang

doi:10.1002/14356007.p05_p01

Cited by 1 publication

(1 citation statement)

References 165 publications

(207 reference statements)

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“…Capillary electrochromatography (CEC) is considered to combine the advantages of the high separation efficiency of CE and the selectivity supplied by liquid chromatography [5,6]. It is important to realize that the CEC system operates with small sample quantities (nanolitre amounts) and sample vials used for the introduction of the sample only require microlitre quantities (typically greater than or equal to 50 µl), which is quite attractive for precious biological samples [7]. Several CEC studies on SA separations have been also reported by Wang & Ye [8] and Krivohlavek et al [9].…”

Section: Introductionmentioning

confidence: 99%

Determination of sulfamerazine in aquatic products by molecularly imprinted capillary electrochromatography

Qin

Jin²,

Gao

et al. 2019

R. Soc. open sci.

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A molecularly imprinted monolith was prepared and evaluated for the special selective separation of sulfamerazine (SMR) by capillary electrochromatography (CEC). The single-step in situ polymerization method was applied through thermally immobilized vinyl groups of itaconic acid and a derivatization capillary column using SMR as the template. The monolith with optimal selectivity and permeability was performed at 45°C for 7 h according to the molar ratios of 1 : 4 : 10 (template/functional monomer/cross-linker). Under the optimized separation conditions of 75% acetonitrile in 20 mM phosphate buffer with pH 5.0, 15 kV applied voltage and 20°C column temperature, the imprinted monolith showed strong recognition ability for SMR and high column performance. Finally, the molecularly imprinted monolith coupled with the CEC method was successfully developed for the quantification of SMR in aquatic products, which was properly validated by a good linear relationship, recoveries and limit of detection. The coupling technique of the molecularly imprinted technology and CEC achieved pre-treatment enrichment and separation analysis in only one miniaturized chromatographic column.

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