Stereoisomeric guaiacylglycerol-β-coniferyl aldehyde ether induces distinctive apoptosis by downregulation of MEK/ERK pathway in hepatocellular carcinoma cells

Yao, Guo‐Dong; Wang, Jie; Song, Xiao-Yu; Zhou, Le; Lou, Li‐Li; Zhao, Wen‐Yu; Lin, Bin; Huang, Xiao‐Xiao; Song, Shao‐Jiang

doi:10.1016/j.bioorg.2018.08.033

Cited by 13 publications

(8 citation statements)

References 30 publications

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“…In ES − mode, peak 13 produces an [M-H+FA] − ion at m/ z 581.1, an [M-H-H 2 O] − ion at m/ z 517 and an [M-H-H 2 O-hexose] − ion at m/ z 355 (hexose neutral loss: −162 Da), with the UV spectrum showing a λ max at 277 and 344 nm. These MS and UV characteristics corresponded to the hexoside form of guaiacylglycerol- β -coniferyl aldehyde ether described by Yao et al. (2018) .…”

Section: Resultssupporting

confidence: 60%

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UPLC-HRMS Analysis Revealed the Differential Accumulation of Antioxidant and Anti-Aging Lignans and Neolignans in In Vitro Cultures of Linum usitatissimum L

et al. 2020

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Over the last few decades, methods relating to plant tissue culture have become prevalent within the cosmetic industry. Forecasts predict the cosmetic industry to grow to an annual turnover of around a few hundred billion US dollars. Here we focused on Linum usitatissimum L., a plant that is well-known for its potent cosmetic properties. Following the a) establishment of cell cultures from three distinct initial explant origins (root, hypocotyl, and cotyledon) and b) selection of optimal hormonal concentrations, two in vitro systems (callus vs cell suspensions) were subjected to different light conditions. Phytochemical analysis by UPLC-HRMS not only confirmed high (neo)lignan accumulation capacity of this species with high concentrations of seven newly described (neo)lignans. Evaluation over 30 days revealed strong variations between the two different in vitro systems cultivated under light or dark, in terms of their growth kinetics and phytochemical composition. Additionally, antioxidant (i.e. four different in vitro assays based on hydrogen-atom transfer or electron transfer mechanism) and anti-aging (i.e. four in vitro inhibition potential of the skin remodeling enzymes: elastase, hyaluronidase, collagenase and tyrosinase) properties were evaluated for the two different in vitro systems cultivated under light or dark. A prominent hydrogenatom transfer antioxidant mechanism was illustrated by the DPPH and ABTS assays. Potent tyrosinase and elastase inhibitory activities were also observed, which was strongly influenced by the in vitro system and light conditions. Statistical treatments of the data showed relationship of some (neo)lignans with these biological activities. These results confirmed the accumulation of flax (neo)lignans in different in vitro systems that were subjected to distinct light conditions. Furthermore, we showed the importance of optimizing these parameters for specific applications within the cosmetic industry.

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Section: Resultssupporting

confidence: 60%

“…In ES + mode, peak 13 produces an [M+H- 277 and 344 nm. These MS and UV characteristics corresponded to the hexoside form of guaiacylglycerol-b-coniferyl aldehyde ether described by Yao et al (2018). Consequently, this compound was tentatively assigned as guaiacylglycerol-bconiferyl aldehyde ether hexoside.…”

Section: Identification Of Metabolites By Uplc-dad-mssupporting

confidence: 53%

UPLC-HRMS Analysis Revealed the Differential Accumulation of Antioxidant and Anti-Aging Lignans and Neolignans in In Vitro Cultures of Linum usitatissimum L

et al. 2020

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“…2C). And the expression levels of the p21, Myt1, p-cdc2 (Tyr 15 ) proteins were increased (Fig. 2D).…”

Section: Resultsmentioning

confidence: 92%

“…Up-regulation of p21 inhibited the activation of the cdc2 and Cyclin B 1 complexes, while Myt-1 reduced activity of cdc2 by phosphorylating Tyr 15 leading to G2/M phase in K562 cells. ERK1/2 signal pathways regulate cell growth, proliferation and differentiation and the JNK, p38 signal pathways play important roles in stress responses including in ammation and apoptosis [15][16][17][18]. We detected that the expression levels of anti-apoptosis proteins (Bcl-2, Bcl-xL and Mcl-1) were downregulated and enhanced the ratio of Bax/Bcl-2, Bax/Bcl-xL.…”

Section: Discussionmentioning

confidence: 99%

Harmine Hydrochloride Induced G2/M Phase Cell Cycle Arrest, Autophagy and Apoptosis in Human Leukemia K562 Cells through Modulation of the MAPK Pathway

Liu¹,

Zhang²,

Zhao³

et al. 2021

Preprint

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Background Previous studies have indicated that harmine hydrochloride (HAR-HC) has anti-tumor characteristics. However, its potential impact on human leukemia cells is unknown. In this study, we explored the potential mechanism of HAR-HC effects on human leukemia cells in vitro. Methods MTT assay was used to detect cell viability; A flow cytometer was used to analyze the cell cycle; Anexinn V-FITC/PI was used to detect cell apoptosis; Western blotting assay was used to analyze the expression of related proteins. Results The result of flow cytometry suggested G2/M phage arrest in K562 cells induced by HAR-HC. The expression levels of Cyclin E2, Cyclin D1, Bcl-2, Bcl-xL, Mcl-1, pro-caspase-3, and PARP decreased and the expression levels of Cyclin A2, Cyclin B1, p21, Myt-1, p-cdc2 (Tyr15), cleaved -caspase-3 and cleaved-PARP increased. Moreover, the expression of p-JNK and p-ERK1/2 increased and autophagy was induced in the HAR-HC treatment group. Additionally, HAR-HC facilitated autophagy by activating the ERK1/2 pathway. Conclusion HAR-HC induced G2/M phase cell cycle arrest, autophagy and apoptosis by activating the JNK, and ERK1/2 pathways in the human leukemia K562 cells.

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“…These active materials show anti-inflammatory, antihypertensive and anti-angiogenic properties in humans (8,9). At the same time, recent studies have shown that the monomers extracted from P. quassioides have the ability to increase intracellular reactive oxygen species and induce apoptosis in HepG2 and Hep3B liver cancer cells (16,17). Despite these data, the anti-cancer effect of P. quassioides on cervical cancer and the possible underlying mechanism has not yet been studied.…”

mentioning

confidence: 99%

Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

Gong

Liu

Jin

et al. 2020

In Vivo

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Background/Aim: Picrasma quassioides (P. quassioides) is used in traditional Asian medicine widely for the treatment of anemopyretic cold, eczema, nausea, loss of appetite, diabetes mellitus, hypertension etc. In this study we aimed to understand the effect of P. quassioides ethanol extract on SiHa cervical cancer cell apoptosis. Materials and Methods: The P. quassioides extract-induced apoptosis was analyzed using the MTT assay, fluorescence microscopy, flow cytometry and western blotting. Results: P. quassioides extract induced cellular apoptosis by increasing the accumulation of cellular and mitochondrial reactive oxygen species (ROS) levels and inhibiting ATP synthesis. Pretreatment with N-Acetylcysteine (NAC), a classic antioxidant, decreased the intracellular ROS production and inhibited apoptosis. In addition, the P38 MAPK signaling pathway is a key in the apoptosis of SiHa cells induced by the P. quassioides extract. Conclusion: The P. quassioides extract exerts its anti-cancer properties on SiHa cells through ROS-mitochondria axis and P38 MAPK signaling. Our data provide a new insight for P. quassioides as a therapeutic strategy for cervical cancer treatment. Cervical cancer is the fourth most common cancer affecting women worldwide (1), putting a huge financial and medical burden on society, so it is necessary to work together globally to improve its treatment. At present, the methods of treating cervical cancer mainly include surgery, chemotherapy and adjuvant chemotherapy (2, 3), with certain side effects, such as loss of appetite and weight loss. Screening of natural products from plant sources, such as herb extracts, with minimal side effects that can be obtained in a cost-effective manner have received global attention for treating several types of cancers (4-6). Picrasma quassioides (P. quassioides) is a Asian traditional medicine mainly distributed in Korea and South China (7). The bark, roots, stems and leaves are traditionally used for the treatment of anemopyretic cold, sore throat, dysentery, eczema, nausea, loss of appetite, diabetes mellitus, hypertension and other conditions (8-10). Chemical investigation has shown that alkaloids, including β-carboline (11, 12), canthinone (13) and bis β-carboline alkaloids (14, 1823 This article is freely accessible online.

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Stereoisomeric guaiacylglycerol-β-coniferyl aldehyde ether induces distinctive apoptosis by downregulation of MEK/ERK pathway in hepatocellular carcinoma cells

Cited by 13 publications

References 30 publications

UPLC-HRMS Analysis Revealed the Differential Accumulation of Antioxidant and Anti-Aging Lignans and Neolignans in In Vitro Cultures of Linum usitatissimum L

UPLC-HRMS Analysis Revealed the Differential Accumulation of Antioxidant and Anti-Aging Lignans and Neolignans in In Vitro Cultures of Linum usitatissimum L

Harmine Hydrochloride Induced G2/M Phase Cell Cycle Arrest, Autophagy and Apoptosis in Human Leukemia K562 Cells through Modulation of the MAPK Pathway

Picrasma quassioides Extract Elevates the Cervical Cancer Cell Apoptosis Through ROS-Mitochondrial Axis Activated p38 MAPK Signaling Pathway

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