Stem cells and cardiovascular tissue repair: Mechanism, methods, and clinical applications

Zhang, Qi; Madonna, R; Shi, Weifeng; Perin, Emerson C.; Angeli, Franca S.; Murad, Ferid; Yeh, Edward T.H.; Buja, L. Maximilian; Caterina, Raffaele De; Willerson, James T.; Geng, Yong

doi:10.1016/j.jccr.2005.12.003

Cited by 13 publications

(14 citation statements)

References 88 publications

(98 reference statements)

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“…Our data indicate that this continuously perfused cell culture device prevents the buildup of metabolites. Similar with those reported by others (Webster and Bishopric, 1992;Webster et al, 1995Webster et al, , 1999, the new bioreactor used in this study is a feasible model system for the detection of the biological effects of cardiac preconditioning or reperfusion-injury that was explored previously by Murry et al (2006), Aoki et al (2004), Ball and Levine (2005), Schäffler and Büchler (2007), Roche et al (2007), Zhang et al (2006), Kinnaird et al (2004), Ohnishi et al (2007), and Bingong et al (2007).…”

Section: Determination Of Regional Cell Viability In Different Sides supporting

confidence: 84%

Assessment of fetal canine cardiac myocyte survival in a dual‐side flow bioreactor with modeled hypoxia/reperfusion injury

Moore

Kleis

Geng

2008

Biotech & Bioengineering

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The present study introduces a new experimental model of hypoxia/reperfusion injury using a newly developed bioreactor system. The injury is introduced and kept localized via fluid dynamic manipulation. Using low Reynolds number fluid flow, regions of the culture can be injured while maintaining physiological conditions in the remaining culture. This approach enables both normal and injured cells within the same monolayer to be investigated side-by-side. The current study evaluated the ability of the model to induce localized reperfusion injury in a monolayer of fetal canine cardiomyocytes (FCCs). Significant apoptosis was found in the hypoxia/reperfusion-injured but not normal-flow regions of the myocyte cultures. The model holds the potential to help elucidate the fundamental mechanisms of hypoxic/reperfusion insults in myocardium.

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Section: Determination Of Regional Cell Viability In Different Sides supporting

confidence: 84%

Assessment of fetal canine cardiac myocyte survival in a dual‐side flow bioreactor with modeled hypoxia/reperfusion injury

Moore

Kleis

Geng

2008

Biotech & Bioengineering

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“…Since the developed magnetic PLA nanofilms are intended to be applied in the field of CTE as cell delivery scaffolds, the insertion of these bioscaffolds into the heart has to occur immediately after myocardial infarction to rapidly repair the damaged area. 5,38 For this purpose, the effect of the embedded MNPs on the cytocompatibility of H9c2 cells was evaluated at 24 h after their seeding on the nanofilms. The results obtained using the LIVE/DEADV R assay showed that cells were viable (green fluorescence) on each sample without showing significant apoptotic behavior (red fluorescence) (Fig.…”

Section: Effects Of Magnetic Polymeric Nanofilms On Cell Proliferationmentioning

confidence: 99%

Influence of nanoparticle‐embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation

Ventrelli

Fujie

Turco

et al. 2013

J Biomedical Materials Res

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The development of functional substrates to direct cellular organization is important for biomedical applications such as regenerative medicine and biorobotics. In this study, we prepared freestanding polymeric ultrathin films (nanofilms) consisting of poly(lactic acid) (PLA) and magnetic nanoparticles (MNPs), and evaluated the effects of their surface properties on the organization of cardiac-like rat myoblasts (H9c2). We changed surface properties of the PLA nanofilms (i.e., roughness and wettability) as a function of MNPs concentration. We found that the incorporation of MNPs into the nanofilms enhanced both proliferation and adhesion of H9c2 cells. Through the morphological assessment of the differentiated H9c2 cells, we also found that the presence of MNPs significantly increased the fusion index and the surface area of myotubes. In conclusion, the embedding of MNPs is a simple method to tailor the physicochemical properties of the polymeric nanofilms, yet it is an effective approach to enhance the cellular morphogenesis in the field of cardiac tissue engineering for regenerative medicine and biorobotics applications.

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“…Although these possibilities are supported by much evidences, direct proof of the possibilities remains to be done in the adipose tissue in obesity. Zhang et al (2006) and Barry and Murphy (2004) stated that stem cells could be classified in embryo, or adult cells, depending on their origin and biological properties. The embryonic stem cells are derived from the inner layer of the blastocyst stage embryo and have the capability to origin several cell lineages.…”

Section: Inflammationmentioning

confidence: 99%

Mesenchymal stem cells: emphasis in adipose tissue

Bertassoli

Neto

Oliveira

et al. 2013

Braz. arch. biol. technol.

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Stem cells and cardiovascular tissue repair: Mechanism, methods, and clinical applications

Cited by 13 publications

References 88 publications

Assessment of fetal canine cardiac myocyte survival in a dual‐side flow bioreactor with modeled hypoxia/reperfusion injury

Assessment of fetal canine cardiac myocyte survival in a dual‐side flow bioreactor with modeled hypoxia/reperfusion injury

Influence of nanoparticle‐embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation

Mesenchymal stem cells: emphasis in adipose tissue

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