Influence of nanoparticle‐embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation

Abstract

The development of functional substrates to direct cellular organization is important for biomedical applications such as regenerative medicine and biorobotics. In this study, we prepared freestanding polymeric ultrathin films (nanofilms) consisting of poly(lactic acid) (PLA) and magnetic nanoparticles (MNPs), and evaluated the effects of their surface properties on the organization of cardiac-like rat myoblasts (H9c2). We changed surface properties of the PLA nanofilms (i.e., roughness and wettability) as a function of MNPs concentration. We found that the incorporation of MNPs into the nanofilms enhanced both proliferation and adhesion of H9c2 cells. Through the morphological assessment of the differentiated H9c2 cells, we also found that the presence of MNPs significantly increased the fusion index and the surface area of myotubes. In conclusion, the embedding of MNPs is a simple method to tailor the physicochemical properties of the polymeric nanofilms, yet it is an effective approach to enhance the cellular morphogenesis in the field of cardiac tissue engineering for regenerative medicine and biorobotics applications.

On the injectability of free-standing magnetic nanofilms

“…Recently, biodegradable and biocompatible materials (e.g. polysaccharides or polyesters) have been proposed as soft patches for cosmetic use, as innovative alternative to traditional surgical sutures, as nanopatches on gastrointestinal wall or as flexible cell growth supports , Fujie 2007, Fujie 2009, Pensabene 2009, Ricotti 2010, Fujie 2012, Ventrelli 2014. In particular, it has been demonstrated that free-standing poly(Llactic acid) (PLLA) nanofilms can adhere tightly to skin or organs by physical adhesion (i.e.…”

Iterative Cellular Screening System for Nanoparticle Safety Testing

“…However, the proliferation rate of adherent cells can be affected by nanostructured surfaces. Several studies have demonstrated that the surface roughness of the cell culture plates influences the cellular behavior [28][29][30]. Therefore, the stability of certain nanoparticle coatings (nanomaterials and nanocomposites) and the effect of potentially solved nanoparticles to the human organism may be more relevant.…”

Self-assembled Monolayers and Nanocomposite Hydrogels of Functional Nanomaterials for Tissue Engineering Applications

“…Additionally, nanofilms with MNPs showed better differentiation of cells compared to the absence of MNPs. Myotubes formation together with higher fusion index was obtained in the presence of MNPs . Similarly the micropillar patterns of polymer/Fe 3 O 4 NPs nanocomposites were fabricated via thermal embossing micro‐imprint lithography.…”

“…Myotubes formation together with higher fusion index was obtained in the presence of MNPs. [69] Similarly the micropillar patterns of polymer/Fe 3 O 4 NPs nanocomposites were fabricated via thermal embossing micro-imprint lithography. The polymer nanocomposite showed shape memory function which can be triggered by external stimuli such as magnetic field or heat.…”