Stem cells and adipose tissue engineering

Gomillion, Cheryl T.; Burg, Karen J. L.

doi:10.1016/j.biomaterials.2006.07.033

Cited by 295 publications

(223 citation statements)

References 112 publications

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“…6 Preadipocytes are considered more advantageous than mature adipocytes in adipose tissue engineering, owing to their proliferative capabilities. 7 As a result, exhaustive exhibition and purposeful control of preadipocytes differentiation will also have a role in repairing adipose tissue defects.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of angiotensinogen by shRNA-mediated RNA interference inhibits human visceral preadipocytes differentiation

Jiang

2009

Int J Obes

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Objective: The immediate cause of obesity is the massive deposition of subcutaneous and visceral fat attributing to the continuous proliferation and differentiation of preadipocytes. The identification of the underlying molecular mechanisms of preadipocytes differentiation is urgent, and will have an important role in plastic and reconstructive surgical procedures. Methods: Two small hairpin RNA (shRNA)-mediated RNA interference plasmids have been constructed on the basis of the activity of H1 promoter-driven expression vector psiRNA-hH1neo to suppress the expression of angiotensinogen (AGT) in human preadipocytes-visceral (HPA-v). Subsequently, glycerol-3-phosphate dehydrogenase (G3PDH) activity and intracytoplasmic lipids content were detected during the process of HPA-v differentiation. Results: Small hairpin RNA-expressing vectors have been successfully constructed to suppress the expression of AGT significantly. Both intracytoplasmic lipids content and G3PDH activity decreased to a certain extent compared with that in the control group in the whole process of HPA-v differentiation. Conclusions: Two shRNA-mediated AGT-targeting plasmids inhibited the process of HPA-v differentiation to a certain extent. However, the accumulation of intracytoplasmic lipids was not exclusively determined by the expression of AGT, and it may also be regulated by other factors. In conclusion, this study provided a method to inhibit the process of preadipocytes differentiation, and it may have a role in obesity treatment and adipose tissue engineering application.

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Section: Introductionmentioning

confidence: 99%

Knockdown of angiotensinogen by shRNA-mediated RNA interference inhibits human visceral preadipocytes differentiation

Jiang

2009

Int J Obes

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“…We have previously used such computational models to provide support for the hypothesis that stem cell fate during fracture healing is governed by the local oxygen concentration and stiffness of the surrounding substrate (Burke and Kelly, 2012;Burke et al, 2013Burke et al, , 2014. This algorithm was developed based on in-vitro observations which demonstrate the fundamental role played by substrate stiffness in regulating MSC lineage commitment (Engler et al, 2006;Huebsch et al, 2010;Young et al, 2013;Park et al, 2011), and by experiments demonstrating that hypoxia inhibits osteogenesis and adipogenesis while promoting chondrogenesis (Kanichai et al, 2008;Cao, 2007;Hankenson et al, 2011;Peiffer et al, 2011;Hirao et al, 2006;Street et al, 2002;Gomillion and Burg, 2006;Hausman and Richardson, 2004). This computational model did not however propose a specific hypothesis for how environmental factors regulate the initiation and progression of chondrocyte hypertrophy and endochondral ossification, which is central to successful secondary fracture healing.…”

Section: Introductionmentioning

confidence: 99%

A computational model to explore the role of angiogenic impairment on endochondral ossification during fracture healing

O'Reilly

Hankenson

Kelly

2016

Biomech Model Mechanobiol

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While it is well established that an adequate blood supply is critical to successful bone regeneration, it remains poorly understood how progenitor cell fate is affected by the altered conditions present in fractures with disrupted vasculature. In this study, computational models were used to explore how angiogenic impairment impacts oxygen availability within a fracture callus and hence regulates mesenchymal stem cell (MSC) differentiation and bone regeneration. Tissue differentiation was predicted using a previously developed algorithm which assumed that MSC fate is governed by the local oxygen tension and substrate stiffness. This was updated to include conditions for oxygen regu- lated cell death and endochondral ossification. The updated algorithm was validated by using it within a model of normal fracture healing where it predicted the experimentally observed quantity and spatial distribution of bone and cartilage at 10 and 20 days post fracture (dpf). Furthermore, it also predicted the ratio of cartilage which had become hypertrophic at 10 dpf. Following this, three models of fracture healing with increasing levels of angiogenic impairment were developed. Under mild impairment the model predicted the experimentally observed reduction in hypertrophic cartilage at 10 dpf as well as the persistence of cartilage at 20 dpf. Models of more severe angiogenic impairment predicted the development of fibrous and necrotic tissue within the callus. These results provide insight into how environmental factors specific to an ischemic callus regulate tissue regeneration and provide support for the hypothesis that endochondral ossification during fracture healing is governed by the local oxygen tension.

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“…MSC has the unusual capacity for self-renewal and differentiation, which has a high therapeutic value in reprogramming growth of tissues to repair injuries and treat diseases (Alonso and Fuchs 2003). Another important property of MSC is to generate the cell types of tissues completely different from its origin, a phenomenon called plasticity (Gomillion and Burg 2006;Fuchs 2008). Recent studies have shown that MSC from the bone marrow can differentiate into various cells such as ectodermal, mesodermal and endodermal when transplanted into the body, repairing surgically induced wounds and burn injuries (Liu et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of rat plasma proteins after bothropic venom inoculation and treatment with Mesenquimal stem cells

Telles

Michel

Lucia

et al. 2014

Braz. arch. biol. technol.

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Stem cells and adipose tissue engineering

Cited by 295 publications

References 112 publications

Knockdown of angiotensinogen by shRNA-mediated RNA interference inhibits human visceral preadipocytes differentiation

Knockdown of angiotensinogen by shRNA-mediated RNA interference inhibits human visceral preadipocytes differentiation

A computational model to explore the role of angiogenic impairment on endochondral ossification during fracture healing

Evaluation of rat plasma proteins after bothropic venom inoculation and treatment with Mesenquimal stem cells

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