Stem Cell Therapy in Spinal Cord Injury: In Vivo and Postmortem Tracking of Bone Marrow Mononuclear or Mesenchymal Stem Cells

Özdemi̇r, Mevci̇; Attar, Ayhan; Kuzu, Işınsu; Ayten, Murat; Özgencil, Enver; Bozkurt, Melih; Dalva, Klara; Uçkan, Duygu; Kılıç, Emine; Sancak, Tanzer; Kanpolat, Yücel; Beksaç, Meral

doi:10.1007/s12015-012-9376-5

Cited by 30 publications

(15 citation statements)

References 37 publications

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“…The ensuing progressive decline of the bioluminescent signal during the second post-transplant week may reflect the decrease in transplanted cell mass. These observations are in accordance with previously reported gradual decrease in bioluminescence signal during the first week after transplantation of MSCs in a spinal cord injury model [32] and in transplanted pancreatic islets [33]. The reduction of isogeneic transplanted mass during the first 2 weeks was previously documented in an animal study [34, 35] and also in clinics [36].…”

Section: Discussionsupporting

confidence: 92%

Multimodal Imaging Reveals Improvement of Blood Supply to an Artificial Cell Transplant Site Induced by Bioluminescent Mesenchymal Stem Cells

et al. 2016

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PurposeAn artificial site for cell or pancreatic islet transplantation can be created using a polymeric scaffold, even though it suffers subcutaneously from improper vascularisation. A sufficient blood supply is crucial for graft survival and function and can be enhanced by transplantation of mesenchymal stem cells (MSCs). The purpose of this study was to assess the effect of syngeneic MSCs on neoangiogenesis and cell engraftment in an artificial site by multimodal imaging.ProceduresMSCs expressing a gene for luciferase were injected into the artificial subcutaneous site 7 days after scaffold implantation. MRI experiments (anatomical and dynamic contrast-enhanced images) were performed on a 4.7-T scanner using gradient echo sequences. Bioluminescent images were acquired on an IVIS Lumina optical imager. Longitudinal examination was performed for 2 months, and one animal was monitored for 16 months.ResultsWe confirmed the long-term presence (lasting more than 16 months) of viable donor cells inside the scaffolds using bioluminescence imaging with an optical signal peak appearing on day 3 after MSC implantation. When compared to controls, the tissue perfusion and vessel permeability in the scaffolds were significantly improved at the site with MSCs with a maximal peak on day 9 after MSC transplantation.ConclusionsOur data suggest that the maximal signal obtained by bioluminescence and magnetic resonance imaging from an artificially created site between 3 and 9 days after MSC transplantation can predict the optimal time range for subsequent cellular or tissue transplantation, including pancreatic islets.

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Section: Discussionsupporting

confidence: 92%

Multimodal Imaging Reveals Improvement of Blood Supply to an Artificial Cell Transplant Site Induced by Bioluminescent Mesenchymal Stem Cells

et al. 2016

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“…Such characteristics may have facilitated the growth of regenerating axons originated from the lesioned root, as indicated by Figure 10 (white dotted circles). Mononuclear cells were used based on the neuroprotective role in peripheral nerve regeneration (Goel et al, 2009) and because the ability to modulate inflamatory response by releasing anti-inflammatory cytokines (Ozdemir et al, 2012). Mononuclear cells are also easy to obtain, with no need of culturing (Goel et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Synaptic plasticity and sensory-motor improvement following fibrin sealant dorsal root reimplantation and mononuclear cell therapy

et al. 2014

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Root lesions may affect both dorsal and ventral roots. However, due to the possibility of generating further inflammation and neuropathic pain, surgical procedures do not prioritize the repair of the afferent component. The loss of such sensorial input directly disturbs the spinal circuits thus affecting the functionality of the injuried limb. The present study evaluated the motor and sensory improvement following dorsal root reimplantation with fibrin sealant (FS) plus bone marrow mononuclear cells (MC) after dorsal rhizotomy. MC were used to enhance the repair process. We also analyzed changes in the glial response and synaptic circuits within the spinal cord. Female Lewis rats (6–8 weeks old) were divided in three groups: rhizotomy (RZ group), rhizotomy repaired with FS (RZ+FS group) and rhizotomy repaired with FS and MC (RZ+FS+MC group). The behavioral tests electronic von-Frey and Walking track test were carried out. For immunohistochemistry we used markers to detect different synapse profiles as well as glial reaction. The behavioral results showed a significant decrease in sensory and motor function after lesion. The reimplantation decreased glial reaction and improved synaptic plasticity of afferent inputs. Cell therapy further enhanced the rewiring process. In addition, both reimplanted groups presented twice as much motor control compared to the non-treated group. In conclusion, the reimplantation with FS and MC is efficient and may be considered an approach to improve sensory-motor recovery following dorsal rhizotomy.

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“…İkinci çalışma ise 13 amiyotrifil lateral skleroz hastasına C1-2'den laminektomi yapılarak direkt beyin sapına Kİ kökenli MNH uygulanmasıdır. [19] Yakın zamanlarda hayvanlarda iskemik kolon anastomozu modelinde Kİ kökenli MKH kullanımı Adaş ve ark. [20] tarafından yayınlanmıştır.…”

Section: Nörolojik Bilimlerunclassified

Clinical use of hematopoietic stem cells

Arat¹

2016

FNG & Bilim Tıp Transplantasyon Dergisi

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Hematopoietic stem cells (HSC) have been used clinically since the 1950s. Towards the seventies, they have been started to be used routinely for therapeutic purpose in benign disorders of bone marrow (e.g. acquired severe aplastic anemia, Fanconi anemia, Thalassemia major, sickle cell anemia) and for postremission treatment consolidation purpose in malign disorders of bone marrow. Currently, 10 to 80 thousand HSC transplantations are performed annually and the one millionth transplantation was performed recently. Regarding the procedures performed in our country, the number of transplantation centers doubled during the last five years and the total number of transplantations reached 3,000s. The success of this universal and standard treatment method has accelerated studies on regenerative medicine. In this respect, cardiology has led the way, followed by neurological sciences and liver disorders and it was observed that the clinical use of bone marrow or peripheral blood HSCs for experimental purpose in various disorders has increased. In our country, a limited number of clinical research has been conducted on this subject. Hereof, while there is no development involving the standard clinical use of adult stem cells except for mesenchymal stem cells (MSC), researches on MSC and other adult and embryonic stem cells in regenerative medicine are ongoing at full speed. In our country, while HSC transplantation is performed in published standard universal indications and reimbursed, usage of non-hematologic adult HSCs is subject to legal permission from Turkish Republic's Ministry of Health. On the other hand, clinical use of embryonic stem cells is forbidden completely. In this review, we aimed to overview HSC based regenerative medicine approaches conducted in our country in terms of standard clinical use, indications, types, and frequent complications.

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Stem Cell Therapy in Spinal Cord Injury: In Vivo and Postmortem Tracking of Bone Marrow Mononuclear or Mesenchymal Stem Cells

Cited by 30 publications

References 37 publications

Multimodal Imaging Reveals Improvement of Blood Supply to an Artificial Cell Transplant Site Induced by Bioluminescent Mesenchymal Stem Cells

Multimodal Imaging Reveals Improvement of Blood Supply to an Artificial Cell Transplant Site Induced by Bioluminescent Mesenchymal Stem Cells

Synaptic plasticity and sensory-motor improvement following fibrin sealant dorsal root reimplantation and mononuclear cell therapy

Clinical use of hematopoietic stem cells

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