Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

Abstract

In the present research, statistical PlackettÀBurman design (PBD) and central composite design (CCD) were used to optimize the medium's components in order to enhance the chitinase activity of Pseudomonas fluorescens strain HN1205. Among the seven nutritional elements that were studied, crab shell powder, CaCl 2 ¢2H 2 O and yeast extract were proved using PBD to have significant effect on chitinase activity. An optimal medium was obtained by applying a three-factor central composite design, which consisted of crab shell powder (1.0 g/L), CaCl 2 (0.5 g/L) and yeast extract (0.5 g/L), with the highest chitinase activity of 1.03 U/mL. This value was 2.87-fold higher than the activity obtained in the lowest productive medium in the design matrix. The chitinase produced by the HN1205 strain was partially purified with 80% ammonium sulphate and anion-exchange chromatography and assessed for inhibition of hatching of nematode eggs. The partially purified chitinase significantly reduced the hatch of Meloidogyne incognita eggs (8.1%), as compared to the effect of 10 mmol/L Tris-HCl buffer (pH 8.0) (49.4%) and boiled chitinase (54.7%). This study demonstrated the role of chitinolytic enzymes produced by P. fluorescens strain HN1205. The obtained optimal activity proved that the enzymes could be potential biological control agents.

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

“…AD showed maximum chitinase synthesis when ammonium sulfate was used as a nitrogen source 44 , while sodium nitrate was ideal for the production of chitinase in Stachybotrys elegans 45 . In the present study, KH 2 PO 4 and MgSO 4 showed a positive impact on chitinase production, whereas, their influence was insignificant during chitinase production by Pseudomonas fluorescens strain HN1205 46 .…”

“…CH-2 51 Fish market soil – 3.1 (PBD and CCD) Paenibacillus sp. 44 Coastal soil 3.84 20.01 (PBD and CCD) Paenibacillus elgii 52 Marine soil 3.157 24.53 Pseudomonas fluorescens strain HN1205 46 Coastal soil – 1.03 U/mL(PBD and CCD) Paenibacillus sp. D1 38 – 93.2 (PBD and CCD) …”

Process Optimization on Chitinase Production by Locally Isolated Enterobacter sp. and Zymomonas sp.

“…This indicates that different strain of bacteria able to tolerate different concentration of colloidal chitin for the production of chitinase. These were proved by different researchers [3], [19], [20] i.e. [21] reported the maximum chitinase production was at 3 g/L of colloidal chitin.…”

Potential of Chitinolytic Bacillus amyloliquefaciens SAHA 12.07 and Serratia marcescens KAHN 15.12 as Biocontrol Agents of Ganoderma boninense

“…Chitinase can be produced by bacteria, fungi, insects and plants. But the production and application of chitinases from bacteria has been usedwidely (Bhattacharya et al, 2007;Ilyina et al, 2013;Lee and Kim, 2015). Previously research reported that chitinolytic bacteria have been screened from soil of oil palm plantation in Jambi Province, Indonesia and the chitinase could inhibit the growth of Curvularia affinis and Colletotrichum gloeosporioides causing leaf blight disease on oil palm (Asril et al, 2014).…”

“…The content of chitin in the mycelium of the phylum Basidiomycota approximately 26.4-64.5% (Di Mario et al, 2008), whereas in Ganoderma lucidum is 41% (Alvarez et al, 2014). Chitin can be degraded enzymatically by chitinase which function as biocontrol in fungal pathogens (Singh et al, 1999;Dai et al, 2011) and insect pathogens Nurdebyandaru et al, 2010;Tu et al, 2010;Lee and Kim, 2015).…”