Potential of Chitinolytic Bacillus amyloliquefaciens SAHA 12.07 and Serratia marcescens KAHN 15.12 as Biocontrol Agents of Ganoderma boninense

Abstract

The objective of this research was to screen and identify of chitinolytic bacteria which have an ability to inhibit the growth of G. boninense and also to characterize its chitinase activity on degrading the chitin of G. boninense. Screening of chitinolytic bacteria against G. boninense by using dual culture method on Potato Dextrose Agar (PDA) showed that bacterial isolates SAHA 12.07 and KAHN 15.12 had the highest percentage inhibition of 68,19 and 40, 29%, respectively. Based on 16S rRNA identifications, SAHA 12.07 and KAHN 15.12 were identified as Bacillus amyloliquefaciens and Serratia marcescens, respectively. Isolate SAHA 12.07 produced optimum chitinase at 48 h and KAHN 15.12 at 54 h of incubation in chitin medium. Crude chitinase of SAHA 12.07 and KAHN 15.12 have precipitated by acetone at 60 and 20%, respectively. Chitinase activity had active at pH 4-10 and temperature about 20-80°C. In vitro lysis of G. boninense bioassay showed that the chitinase of SAHA 12.07 and KAHN 15.12 were capable to inhibit the growth of mycelium G. boninense and could release of N-acetylglucosamine by incubation of G. boninense with partially purified enzyme.

Ganoderma boninense: general characteristics of pathogenicity and methods of control

“…and Trichoderma spp. ( Bakhtiar et al., 2010 ; Sundram et al., 2011 ; Azizah et al., 2015 ; Goh et al., 2015 ; Chow et al., 2017 ; Lim et al., 2018 ; Chow et al., 2019 ; Sujarit et al., 2020 ). Inhibition happened attributed to some potential antimicrobials compound such as eudistomin I, Gly-Met-OH, halstoctacosanolide A, lovastatin, naringenin, N-acetyl-leu-leu-tyr-amide, N-methyl-a-aminoisobutyric acid, penipanoid A, phenazine and phenazine-1-carboxylic acid, phenylethyl alcohol, phenyl alkenoic acids, pyrene-1,6-dione, 4-O-8’,5”-5’-dehydrotriferulic acid, 7-O-beta-D-glucoside, 12-deoxyaklanonic acid, and 12-oxo-10Z-dodecenoic acid ( Angel et al., 2016 ; Alexander et al., 2017b ; Lee et al., 2018 ; Ahmad et al., 2020 ; Sujarit et al., 2020 ).…”

Lactic acid bacteria administration from Jember tempeh (Indonesia) as a probiotic candidate in intestinal physiology and histology of mice strain Balb-C

“…The 10% LAB inoculum was cultured in 100 ml of GYP broth. Cultures were collected every 1 to 24 h, and their optical density was measured spectrophotometrically at 600 nm (Azizah et al 2015).…”

“…The LAB pellet was washed twice with phosphate buffer (pH 7). Suspension with LAB cell was concentrated at 10 -8 dilution level with the 7 Mc Farland (2.1×10 8 cel/mL) method using phosphate buffer (pH 7) as a solvent (Azizah et al 2015).…”

“…The TK.1 and TK.2 isolates showed a midlogarithmic phase at the 6th hour, while TK.4 at the 8 th hour, characterized by a faster increase in cell absorbance and a decrease in pH to 3. In this phase, Azizah et al (2015) stated that the logarithmic number of cells increases rapidly up to a certain limit until it enters the static phase, maximum cell metabolism, and rapid synthesis of cell materials in constant amounts until nutrients are fully used up. This result is in accordance with the previous study results, namely TA.1, TB.1, TK1, TK.2, and TK.4 isolates.…”

Application strategies by selective medium treated with entomopathogenic bacteria Serratia marcescens and Pseudomonas aeruginosa as potential biocontrol against Coptotermes curvignathus

“…Active enzymes (metalloproteases alkaline proteinase, Prt protease) degrade insect tissues during bioconversion of peptides and amino acids to smaller sulfur, nitrogen and phosphorus compounds [19]. Some selected strains of S. marcescens and P. aeruginosa have also been found to reduce plant disease severity to a desirable extent using specific application strategies [20][21][22][23][24][25][26][27][28].…”