Application strategies by selective medium treated with entomopathogenic bacteria
 Serratia marcescens
 and
 Pseudomonas aeruginosa
 as potential biocontrol against
 Coptotermes curvignathus

Chin, Kit Ling; H’ng, Paik San; Lee, Chuan Li; Wong, W. Z.; Go, Wen Ze; Khoo, Pui San; Luqman, Abdullah Chuah; Ashaari, Zaidon

doi:10.1098/rsos.201311

Cited by 4 publications

(3 citation statements)

References 46 publications

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“…Serratia bacteria are regarded as entomopathogenic bacteria, which are used as environmentally friendly biocontrol agents against various insect pests. Wood or soil treated with Serratia marcescens at concentrations higher than 10 9 CFU/mL creates a high toxicity and repellent barrier for termites . The oral ingestion of S.…”

Section: Resultsmentioning

confidence: 99%

Biotoxicity Evaluation of a Cationic Star Polymer on a Predatory Ladybird and Cooperative Pest Control by Polymer-Delivered Pesticides and Ladybird

Dong

Chen

Lin

et al. 2022

ACS Appl. Mater. Interfaces

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Although employing nanocarriers for gene/drug delivery shows great potential in agricultural fields, the biotoxicity of nanocarriers is a major concern for largescale applications. Herein, we synthesized a cationic star polymer (SPc) as a pesticide nanocarrier/adjuvant to evaluate its safety against a widely used predatory ladybird (Harmonia axyridis). The application of SPc at extremely high concentrations nearly did not influence the hatching of ladybird eggs but it led to the death of ladybird larvae at lethal concentration 50 (LC 50 ) values of 43.96 and 19.85 mg/mL through the soaking and feeding methods, respectively. The oral feeding of SPc downregulated many membrane protein genes and lysosome genes significantly, and the cell membrane and nucleus in gut tissues were remarkably damaged by SPc application, revealing that the lethal mechanism might be SPc-mediated membrane damage. Furthermore, the oral feeding of SPc increased the relative abundance of Serratia bacteria in ladybird guts to result in bacterial infection. Coapplication of ladybird and SPc-loaded thiamethoxam/matrine achieved desired control efficacies of more than 80% against green peach aphids, revealing that the coapplication could overcome the slow-acting property of ladybirds. To our knowledge, this is the first attempt to investigate the polymer-mediated lethal mechanism toward natural enemies and explore the possibility of coapplying SPc-loaded pesticides and natural enemies for pest management.

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Section: Resultsmentioning

confidence: 99%

Biotoxicity Evaluation of a Cationic Star Polymer on a Predatory Ladybird and Cooperative Pest Control by Polymer-Delivered Pesticides and Ladybird

Dong

Chen

Lin

et al. 2022

ACS Appl. Mater. Interfaces

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“…S. marcescens can produce a variety of exoenzymes, including chitinase, which can hydrolyse and destroy the surface and periplasmic structure of insects, causing insect death eventually ( Zhao et al, 2020 ; Chin et al, 2021 ). Studies have found that S. marcescens has pathogenicity against a variety of Coleoptera insects.…”

Section: Discussionmentioning

confidence: 99%

Virulence of entomopathogenic bacteria Serratia marcescens against the red palm weevil, Rhynchophorus ferrugineus (Olivier)

Zhong,

Lv,

et al. 2023

PeerJ

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Background The red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier), is an important quarantine pest, which has caused serious economic losses in various palm species, such as coconut, oil palm and date palm. Finding effective biocontrol resources is important for the control of this pest and the protection of palm crops. Methods A pathogenic strain HJ-01 was isolated from infected and dead pupa of Tenebrio molitor using tissue separation method. The HJ-01 strain was streak cultured and purified, and its morphological, physiological, biochemical characteristics, and 16S rDNA homology were identified after conducting a pathogenicity test on RPW larvae. Results Strain HJ-01 exhibited remarkable pathogenicity against RPW larvae. Under the concentration of HJ-01 suspension was 1.0 × 108 cfu/mL, the mortality rate of RPW reached 82.22%, and the half-lethal time (LT50) was 4.72 days. RPW larvae infected with strain HJ-01 showed reduced movement, decreased appetite, and eventual death. As the treatment progresses, the larvae’s bodies turned red, became soft, and started to rot, resulting in the discharge of liquid. HJ-01 demonstrated the ability to produce scarlet pigment after 24 h of culture on a basic medium. Colonies of HJ-01 appeared convex, bright red, moist, and viscous, opaque in the center, irregular at the edges, and emitted an unpleasant odor. Under microscopic observation, the cells of HJ-01 appeared as short rod-shaped and flagellate, with a size ranging from (1.2–1.8) μm × (1.0–1.2) μm. Genomic DNA extraction was performed on the strain, and the 16S rDNA sequence was amplified, yielding a sequence length of 1445 bp. The sequence of strain HJ-01 displayed a 99.72% similarity to that of Serratia marcescens. Phylogenetic tree analysis further confirmed that strain HJ-01 belonged to S. marcescens. Therefore, the strain HJ-01 has a very good lethal effect on RPW larvae, and it may be used as an effective bacterium for the control of RPW.

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“…Wright et al found that M. anisopliae can quickly kill Coptotermes formosanus [ 7 ]. High concentrations of Serratia marcescens and Pseudomonas aeruginosa cause high mortality of Coptotermes curvignathus [ 8 ]. Rhizobacteria can kill O. obesus via the production of harmful hydrogen cyanide in vitro [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Immune Defense Mechanism of Reticulitermes chinensis Snyder (Blattodea: Isoptera) against Serratia marcescens Bizio

Luo

Wang

Tang

et al. 2022

Insects

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Reticulitermes chinensis Snyder is an important pest species in China. Serratia marcescens Bizio (SM1) is a potent biological bacterium. In our lab, we found that SM1 can kill R. chinensis. To date, the interaction between R. chinensis and SM1 has not been studied. Here, we explored immune responses of R. chinensis against SM1 using transcriptome sequencing. To elucidate immune-related genes, we identified 126,153 unigenes from R. chinensis. In total, 178 immune-related differentially expressed genes (DEGs) were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that many cellular responses were enriched in the top 20 terms. Then, we systematically analyzed several cellular immune pathways involved in the response of R. chinensis to SM1, including phagocytosis, autophagy, and endocytosis pathways. Furthermore, the expression profiles of the cellular immune-related genes were assessed using quantitative reverse-transcription PCR, and the expression levels of the selected genes were upregulated. Further results revealed SM1-mediated activation of humoral immune responses genes, including Toll, IMD, and melanization pathways, which suggested the involvement of humoral immune responses in the defense against SM1. This research elucidated the mechanisms underlying the immune defense of R. chinensis against SM1, providing a solid theoretical basis for exploiting new immune suppressive agents to control R. chinensis. Moreover, this study will facilitate the better control of R. chinensis using SM1.

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Application strategies by selective medium treated with entomopathogenic bacteria Serratia marcescens and Pseudomonas aeruginosa as potential biocontrol against Coptotermes curvignathus

Cited by 4 publications

References 46 publications

Biotoxicity Evaluation of a Cationic Star Polymer on a Predatory Ladybird and Cooperative Pest Control by Polymer-Delivered Pesticides and Ladybird

Biotoxicity Evaluation of a Cationic Star Polymer on a Predatory Ladybird and Cooperative Pest Control by Polymer-Delivered Pesticides and Ladybird

Virulence of entomopathogenic bacteria Serratia marcescens against the red palm weevil, Rhynchophorus ferrugineus (Olivier)

Immune Defense Mechanism of Reticulitermes chinensis Snyder (Blattodea: Isoptera) against Serratia marcescens Bizio

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