Statistical optimization for enhanced bacoside A production in plant cell cultures of Bacopa monnieri

Leonard, J.E.; Seth, Bishwanath; Sahu, Binod Bihari; Singh, Vivek; Patra, Nivedita

doi:10.1007/s11240-017-1373-6

Cited by 18 publications

(14 citation statements)

References 34 publications

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“…This could be due to the decreased cell growth when broccoli SCC were initiated with the lowest cell density used (50 g/L). In fact, inoculum density is a key parameter that can alter biomass accumulation, growth, and the production of metabolites in plant cell cultures. − In this sense, some authors analyzed the ajmalicine production from Catharanthus roseus cell cultures using different inoculum densities, and they observed that the production of ajmalicine was significantly lower when low cell densities were used …”

Section: Resultsmentioning

confidence: 99%

Increased Glucosinolate Production in Brassica oleracea var. italica Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis

Sánchez-Pujante

Sabater-Jara

Belchí-Navarro

et al. 2018

J. Agric. Food Chem.

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In this work, the effect of different elicitors and culture conditions on the production of glucosinolates in broccoli cell cultures was studied. The results showed that 0.5 μM coronatine was the best elicitor for increasing glucosinolate production (205-fold increase over untreated cells after 72 h of treatment). Furthermore, the expression levels of some genes related to the biosynthetic pathway of glucosinolates as well as three Myb transcription factors also have been studied. The highest glucosinolate levels found in coronatine-treated cells were closely correlated with the highest gene expression levels of Cyp79b2, Cyp83b1, St5a, Myb51, and Myb122 after 6 h of treatment. The data shown in this study provide new insight into the key metabolic steps involved in the biosynthesis of glucosinolates, which will be of use for future applications of metabolic engineering techniques in broccoli.

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Section: Resultsmentioning

confidence: 99%

Increased Glucosinolate Production in Brassica oleracea var. italica Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis

Sánchez-Pujante

Sabater-Jara

Belchí-Navarro

et al. 2018

J. Agric. Food Chem.

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“…It is well-known that only the optimal levels of elicitor and precursor concentration increases the in vitro production of desired secondary metabolites. In optimization of medium for metabolite production, Central Composite Design (CCD) and Box-Behnken design (BBD) are the most commonly used designs that obtain optimal response from statistical mathematical modeling of Response Surface Methodology (RSM) [23,24]. Statistically, RSM uses symmetrical experimental designs, explores the effects of single or multiple response variables and optimize these variables to get the best possible response [25].…”

Section: Introductionmentioning

confidence: 99%

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

et al. 2020

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Background: In this study, response surface methodology (RSM) and artificial neural network (ANN) was used to construct the predicted models of linear, quadratic and interactive effects of two independent variables viz. salicylic acid (SA) and chitosan (CS) for the production of amarogentin (I), swertiamarin (II) and mangiferin (III) from shoot cultures of Swertia paniculata Wall. These compounds are the major therapeutic metabolites in the Swertia plant, which have significant role and demand in the pharmaceutical industries. Results: Present study highlighted that different concentrations of SA and CS elicitors substantially influenced the % yield of (I), (II) and (III) compounds in the shoot culture established on modified ½ MS medium (supplemented with 2.22 mM each of BA and KN and 2.54 mM NAA). In RSM, different response variables with linear, quadratic and 2 way interaction model were computed with five-factor-three level full factorial CCD. In ANN modelling, 13 runs of CCD matrix was divided into 3 subsets, with approximate 8:1:1 ratios to train, validate and test. The optimal enhancement of (I) (0.435%), (II) (4.987%) and (III) (4.357%) production was achieved in 14 days treatment in shoot cultures of S. paniculata elicited by 9 mM and 12 mg L − 1 concentrations (SA) and (CS). Conclusions: In optimization study, (I) show 0.170-0.435%; (II) display 1.020-4.987% and (III) upto 2.550-4.357% disparity with varied range of SA (1-20 mM) and CS (1-20 mg L − 1). Overall, optimization of elicitors to promote secoiridoid and xanthone glycoside production with ANN modeling (r 2 = 100%) offered more significant results as compared to RSM (r 2 = 99.8%).

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“…Sakurai et al (1996) reported that an optimal inoculum of 1 g DW L − 1 was identi ed for strawberry cell suspension cultures. The ID for Bacopa monnieri cell suspension culture was optimized using statistical optimization tools, and it was reported that 2 g L − 1 is a suitable ID (Leonard et al 2018). The suspension culture of Arnebia benthamii cells with lower ID (5%, w/v) resulted in a higher growth index (~ 5) than with higher ID (15%, w/v) (Kumar et al 2023).…”

Section: Discussionmentioning

confidence: 99%

A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles

Srivast

2024

Preprint

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Viola odorata plant extracts are extensively used in indigenous medicine to treat infectious diseases. V. odorata's limited availability in nature, due to geographical conditions and adulteration, makes continuous supply of high-quality plant raw material challenging. This results in low-quality plant extracts that can have inconsistent and sometimes adverse effects when used in medicine. As an effective solution, in vitro plant biomass cultivation conserves plant diversity, ensuring high-quality raw materials with uniform quality and quantity. Toward this, the current study focuses on establishing bioprocess strategy to achieve maximum V. odorata biomass productivity up to bioreactor level. In this context, in vitro culture conditions were rationally optimised, focusing upon both statistical optimization and one-factor-at-a-time (OFAT) approach, for improved biomass productivity. In particular, basal medium, carbon and nitrogen source, inoculum density, macronutrient concentration, shaking speed, temperature, pH, light intensity, and plant growth regulators were systematically optimized in order to achieve the maximum biomass. This led to increased biomass production from 10.2 ± 0.8 g DW L-1 to 21.68 ± 0.82 g DW L-1 within 12 days of the batch cultivation period. Optimized culture conditions were implemented in three different types of bioreactors, namely, stirred tank, airlift, and bubble column, to cultivate V. odorata biomass. Of these three methods, cultivation in a stirred tank reactor produced ~19.7 g DW L-1 of maximum biomass within a 12-day batch cultivation period. Further, the metabolite profile of the bioreactor cultivated biomass (alcoholic extract) was compared with that of the natural plant biomass using gas chromatography-mass spectrometry based metabolomics, yielding around 18 key principles (metabolites) that exhibit resemblance with that in the natural source. The antibacterial activity of the plant extract was tested against three bacteria responsible for respiratory tract infections, and their antioxidant activity was compared with that of Vitamin E, wherein the results demonstrated comparable activity. The novelty of this study is in the fact that the V. odorata plant cells were cultivated in bioreactors for the first time for potential medicinal applications. It provides an alternative method for sustainable production of plant biomass, thereby conserving the natural plant sources.

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Statistical optimization for enhanced bacoside A production in plant cell cultures of Bacopa monnieri

Cited by 18 publications

References 34 publications

Increased Glucosinolate Production in Brassica oleracea var. italica Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis

Increased Glucosinolate Production in Brassica oleracea var. italica Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

A rationally optimised batch bioreactor cultivation of Viola odorata plant cells for sustainable production of its key bioactive principles

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