Increased Glucosinolate Production in <i>Brassica oleracea</i> var. <i>italica</i> Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis

Sánchez-Pujante, Pedro Joaquín; Sabater-Jara, Ana Belén; Belchí-Navarro, Sarai; Pedreño, María A.; Almagro, Lorena

doi:10.1021/acs.jafc.8b04298

Cited by 9 publications

(7 citation statements)

References 69 publications

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“…The use of PHEN and DPI applied individually caused a high repression in the expression of CYP79B2 gene, suggesting that JA and ROS play a key role in the wound-induced activation of indolic glucosinolate biosynthesis, whereas ET plays a slight role. These observations agree with previous findings in Brassica species [15,19,20]. For instance, in Arabidopsis, CYP79B2 was reported to be inducible by wounding and by MeJA treat-ment [15].…”

Section: Role Of Ja Et and Ros On The Wound-induced Activation Of Glucosinolate Biosynthetic Genessupporting

confidence: 93%

“…For instance, in Arabidopsis, CYP79B2 was reported to be inducible by wounding and by MeJA treat-ment [15]. Likewise, in broccoli cell culture, CYP79B2 was reported to be highly induced by coronatine treatment (mimicking JA action) and was closely correlated with an increase in GS content [19]. Furthermore, 1-MCP application during postharvest of broccoli was reported to reduce CYP79B2 gene expression during storage [20].…”

Section: Role Of Ja Et and Ros On The Wound-induced Activation Of Glucosinolate Biosynthetic Genesmentioning

confidence: 99%

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Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli

Torres-Contreras

Nair

Senés‐Guerrero

et al. 2021

Plants

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Wounding stress is an effective strategy to induce glucosinolate (GS) biosynthesis in broccoli. However, there is insufficient knowledge on the physiological and molecular mechanisms underlying this stress response. Herein, a chemical-genetic approach was applied to elucidate the role of jasmonic acid (JA), ethylene (ET), and reactive oxygen species (ROS) on the wound-induced biosynthesis of GS. Broccoli was processed into chops to induce wounding stress. Broccoli chops were treated with phenidone (PHEN) and diphenyleneiodonium chloride (DPI) as inhibitors of JA and ROS biosynthesis, respectively, whereas 1-methylcyclopropene (1-MCP) was applied as an inhibitor of ET action. Wounding stress induced the expression of genes related to the biosynthesis of indolic and aliphatic GS, which was correlated with the accumulation of GS and modulated by the inhibitors of signaling molecules applied. Results of gene expression analysis indicated that JA played a key role in the activation of most genes, followed by ROS. Furthermore, except for the CYP79B2 gene, PHEN and 1-MCP synergistically downregulated the expression of GS biosynthetic genes evaluated, showing that the interaction between JA and ET was fundamental to modulate GS biosynthesis. Results presented herein increased our knowledge of the physiological and molecular mechanisms governing the wound-induced biosynthesis of GS in broccoli.

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Section: Role Of Ja Et and Ros On The Wound-induced Activation Of Glucosinolate Biosynthetic Genessupporting

confidence: 93%

Section: Role Of Ja Et and Ros On The Wound-induced Activation Of Glucosinolate Biosynthetic Genesmentioning

confidence: 99%

Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli

Torres-Contreras

Nair

Senés‐Guerrero

et al. 2021

Plants

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“…This induced expression pattern of GSL genes was also consistent with the total GSL content in transgenic lines, which implies that BrPP5.2 can regulate GSL content by modulating the GSL biosynthesis-related genes through yet-to-be-identified mechanisms. Of the upregulated DEGs, the upregulation of MYB28 [ 38 ]; CYP83B1 , MYB122 , and ST5a [ 39 ]; ST5b and ST5c [ 40 ]; MAM3 [ 41 ]; and MAM1 and CYP83A1 [ 42 ] was shown to increase the GSL content. Other genes such as CYP79F1 , BCAT4 , AOP2, UGT74C1, BAT5 [ 43 ], and sulfotransferases ( ST5b , ST5c ) [ 44 ] have been shown to participate in various steps of GSL biosynthesis and its modification.…”

Section: Discussionmentioning

confidence: 99%

BrPP5.2 Overexpression Confers Heat Shock Tolerance in Transgenic Brassica rapa through Inherent Chaperone Activity, Induced Glucosinolate Biosynthesis, and Differential Regulation of Abiotic Stress Response Genes

Muthusamy

Kim

et al. 2021

IJMS

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Plant phosphoprotein phosphatases are ubiquitous and multifarious enzymes that respond to developmental requirements and stress signals through reversible dephosphorylation of target proteins. In this study, we investigated the hitherto unknown functions of Brassica rapa protein phosphatase 5.2 (BrPP5.2) by transgenic overexpression of B. rapa lines. The overexpression of BrPP5.2 in transgenic lines conferred heat shock tolerance in 65–89% of the young transgenic seedlings exposed to 46 °C for 25 min. The examination of purified recombinant BrPP5.2 at different molar ratios efficiently prevented the thermal aggregation of malate dehydrogenase at 42 °C, thus suggesting that BrPP5.2 has inherent chaperone activities. The transcriptomic dynamics of transgenic lines, as determined using RNA-seq, revealed that 997 and 1206 (FDR < 0.05, logFC ≥ 2) genes were up- and down-regulated, as compared to non-transgenic controls. Statistical enrichment analyses revealed abiotic stress response genes, including heat stress response (HSR), showed reduced expression in transgenic lines under optimal growth conditions. However, most of the HSR DEGs were upregulated under high temperature stress (37 °C/1 h) conditions. In addition, the glucosinolate biosynthesis gene expression and total glucosinolate content increased in the transgenic lines. These findings provide a new avenue related to BrPP5.2 downstream genes and their crucial metabolic and heat stress responses in plants.

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“…We also identified and quantified individual GLSs from the whole edible parts of Chinese kale plants demonstrating that the content of all GLSs was significantly enhanced by liquiritin treatment at 500 and 750 ppm (Table ). Similar types of GLSs have been previously identified in Chinese kale by Sánchez‐Pujante et al ., Cuong et al . and Wang et al…”

Section: Discussionmentioning

confidence: 99%

“…Changes in the expression levels of various genes involved in GLS and PH biosynthesis were assessed, according to previous reports . For that purpose, another independent experiment was performed in the greenhouse, including the same treatments and culture conditions described above.…”

Section: Methodsmentioning

confidence: 99%

Liquiritin elicitation can increase the content of medicinally important glucosinolates and phenolic compounds in Chinese kale plants

et al. 2019

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BACKGROUND: Brassica oleracea var. alboglabra (Chinese kale) is an important vegetable grown in southernChina. This study was aimed at searching for environmentally friendly and affordable approaches to increase the production of medicinally relevant glucosinolates and phenolic compounds in Chinese kale plants. For this purpose, the foliar application of liquiritin at 0 (control), 250, 500 and 750 ppm was tested starting from the four-leaf stage and repeated every two weeks until plants were two months old. RESULTS: Foliar application of liquiritin in Chinese kale plants significantly increased glucosinolates and total phenolic content, in a dose-dependent manner. Compared with control plants, 2.3-and 1.9-fold increases in yields of glucosinolates and total phenolic content, respectively, were corroborated in Chinese kale plants treated with 750 ppm of liquiritin. Along with rises in the content of eight different glucosinolates, liquiritin elicitation effectively increased the concentration of glycosilated and acylated flavonoids and hydroxycinnamic acids. The expression of genes involved in glucosinolate and phenolic biosynthesis was significantly higher in liquiritin-treated plants as compared to controls.CONCLUSIONS: Liquiritin elicitation is a feasible and environmentally friendly practice for increasing the production of medicinally important glucosinolates and phenolic compounds in Chinese kale, which may improve this plant's value as a nutraceutical food. This study also contributes to understanding the molecular mechanisms underlying liquiritin elicitation. This is the first report documenting the use of liquiritin for an elicitation purpose in plants.Liquiritin elicitation increases medicinal properties of Chinese kale www.soci.org Identification and quantification of individual GLSs and PHs Alkaline hydrolysisTo eliminate acid moieties, which generate mass spectra coincident with those of rhamnosyl and hexosyl residues and may lead to miss-assignments during mass spectroscopic analysis, alkaline J Sci Food Agric 2020; 100: 1616-1624

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Increased Glucosinolate Production in Brassica oleracea var. italica Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis

Cited by 9 publications

References 69 publications

Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli

Chemical Genetics Applied to Elucidate the Physiological Role of Stress-Signaling Molecules on the Wound-Induced Accumulation of Glucosinolates in Broccoli

BrPP5.2 Overexpression Confers Heat Shock Tolerance in Transgenic Brassica rapa through Inherent Chaperone Activity, Induced Glucosinolate Biosynthesis, and Differential Regulation of Abiotic Stress Response Genes

Liquiritin elicitation can increase the content of medicinally important glucosinolates and phenolic compounds in Chinese kale plants

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