Statistical comparison of Campylobacter jejuni subtypes from human cases and environmental sources

Garrett, Nick; Devane, Megan L.; Hudson, John; Nicol, Carolyn; Ball, Andrew; Klena, John D.; Scholes, Paula; Baker, Michael G; Gilpin, Brent; Savill, M.

doi:10.1111/j.1365-2672.2007.03437.x

Cited by 41 publications

(37 citation statements)

References 36 publications

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“…ST-42 complex and ST-61 complex isolates from cattle in our study were also consistent with reports of cattle-origin C. jejuni from other regions of the United States (33). Multiple molecular genotyping studies have found that Campylobacter isolates from bovine and human hosts frequently have the same genotype by a variety of methods, including pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) typing, flagellin gene typing, and MLST (35)(36)(37)(38)(39)(40). A New Zealand study conducted in a dairy farming area that included comparisons between genotypes of Campylobacter from humans, cattle, and poultry found that cattle-origin isolates had a distribution of subtypes more similar to that of human-origin isolates than poultry-origin isolates (36,41).…”

Section: Discussionsupporting

confidence: 76%

“…Multiple molecular genotyping studies have found that Campylobacter isolates from bovine and human hosts frequently have the same genotype by a variety of methods, including pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) typing, flagellin gene typing, and MLST (35)(36)(37)(38)(39)(40). A New Zealand study conducted in a dairy farming area that included comparisons between genotypes of Campylobacter from humans, cattle, and poultry found that cattle-origin isolates had a distribution of subtypes more similar to that of human-origin isolates than poultry-origin isolates (36,41). A study in Denmark using multiple subtyping methods found that subtype sharing was approximately as frequent between poultry and humans as between cattle and humans (39), and a comparison between human, poultry, and raw milk isolates in Québec, Canada, found that three of 10 clonal complexes included isolates from human, raw milk, or water sources (42).…”

Section: Discussionmentioning

confidence: 99%

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Risk Factors for Campylobacteriosis in Two Washington State Counties with High Numbers of Dairy Farms

et al. 2013

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g Campylobacteriosis is a frequently reported, food-borne, human bacterial disease that can be associated with ruminant reservoirs, although public health messages primarily focus on poultry. In Washington State, the two counties with the highest concentrations of dairy cattle also report the highest incidences of campylobacteriosis. Conditional logistic regression analysis of case-control data from both counties found living or working on a dairy farm (odds ratio .3]) were found to be independent risk factors for disease. Campylobacter jejuni isolates from human (n ‫؍‬ 65), bovine (n ‫؍‬ 28), and retail poultry (n ‫؍‬ 27) sources from the same counties were compared using multilocus sequence typing. These results indicated that sequence types commonly found in human isolates were also commonly found in bovine isolates. These findings suggest that, in areas with high concentrations of dairy cattle, exposure to dairy cattle may be more important than food-borne exposure to poultry products as a risk for campylobacteriosis.[

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Risk Factors for Campylobacteriosis in Two Washington State Counties with High Numbers of Dairy Farms

et al. 2013

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“…The relative contributions of different C. coli sources to the human disease burden were estimated using a number of tests. The similarity between the frequency distribution of human C. coli STs and those of the different sources was estimated using proportional similarity indices (PSI) and their bootstrap CrIs, as previously described (38). The PSI measures the area of intersection between two frequency distributions (39) and ranges between 0 and 1, where 0 indicates no similarity and 1 indicates identical frequency distributions.…”

Section: Methodsmentioning

confidence: 99%

Molecular Epidemiology of Campylobacter coli Strains Isolated from Different Sources in New Zealand between 2005 and 2014

Nohra

Grinberg

Midwinter

et al. 2016

Appl Environ Microbiol

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Campylobacteriosis is one of the most important foodborne diseases worldwide and a significant health burden in New Zealand. Campylobacter jejuni is the predominant species worldwide, accounting for approximately 90% of human cases, followed by Campylobacter coli. Most studies in New Zealand have focused on C. jejuni; hence, the impact of C. coli strains on human health is not well understood. The aim of this study was to genotype C. coli isolates collected in the Manawatu region of New Zealand from clinical cases, fresh poultry meat, ruminant feces, and environmental water sources, between 2005 and 2014, to study their population structure and estimate the contribution of each source to the burden of human disease. Campylobacter isolates were identified by PCR and typed by multilocus sequence typing. C. coli accounted for 2.9% (n ‫؍‬ 47/1,601) of Campylobacter isolates from human clinical cases, 9.6% (n ‫؍‬ 108/1,123) from poultry, 13.4% (n ‫؍‬ 49/364) from ruminants, and 6.4% (n ‫؍‬ 11/171) from water. Molecular subtyping revealed 27 different sequence types (STs), of which 18 belonged to clonal complex ST-828. ST-1581 was the most prevalent C. coli sequence type isolated from both human cases (n ‫؍‬ 12/47) and poultry (n ‫؍‬ 44/110). When classified using cladistics, all sequence types belonged to clade 1 except ST-7774, which belonged to clade 2. ST-854, ST-1590, and ST-4009 were isolated only from human cases and fresh poultry, while ST-3232 was isolated only from human cases and ruminant sources. Modeling indicated ruminants and poultry as the main sources of C. coli human infection. IMPORTANCEWe performed a molecular epidemiological study of Campylobacter coli infection in New Zealand, one of few such studies globally. This study analyzed the population genetic structure of the bacterium and included a probabilistic source attribution model covering different animal and water sources. The results are discussed in a global context.

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“…The value for the PSI ranges from 1 for identical frequency distributions to 0 for distributions with no common types. Bootstrap confidence intervals for this measure were estimated based on the approach developed by Garrett et al (20). Structure 2.2 (15, 36) was used as a frequency-based clustering model to investigate the population structure of C. jejuni in the New Zealand poultry supply.…”

Section: Methodsmentioning

confidence: 99%

Molecular Epidemiology ofCampylobacter jejuniin a Geographically Isolated Country with a Uniquely Structured Poultry Industry

Müllner

Collins-Emerson

Midwinter

et al. 2010

Appl Environ Microbiol

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In New Zealand the number of campylobacteriosis notifications increased markedly between 2000 and 2007. Notably, this country's poultry supply is different than that of many developed countries as the fresh and frozen poultry available at retail are exclusively of domestic origin. To examine the possible link between human cases and poultry, a sentinel surveillance site was established to study the molecular epidemiology of Campylobacter jejuni over a 3-year period from 2005 to 2008 using multilocus sequence typing. Studies showed that 60.1 to 81.4% of retail poultry carcasses from the major suppliers were contaminated with C. jejuni. Differences were detected in the probability and level of contamination and the relative frequency of genotypes for individual poultry suppliers and humans. Some carcasses were contaminated with isolates belonging to more than one sequence type (ST), and there was evidence of both ubiquitous and supplier-associated strains, an epidemiological pattern not recognized yet in other countries. The common poultry STs were also common in human clinical cases, providing evidence that poultry is a major contributor to human infection. Both internationally rare genotypes, such as ST-3069 and ST-474, and common genotypes, such as ST-45 and ST-48, were identified in this study. The dominant human sequence type in New Zealand, ST-474, was found almost exclusively in isolates from one poultry supplier, which provided evidence that C. jejuni has a distinctive molecular epidemiology in this country. These results may be due in part to New Zealand's geographical isolation and its uniquely structured poultry industry.

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Statistical comparison of Campylobacter jejuni subtypes from human cases and environmental sources

Cited by 41 publications

References 36 publications

Risk Factors for Campylobacteriosis in Two Washington State Counties with High Numbers of Dairy Farms

Risk Factors for Campylobacteriosis in Two Washington State Counties with High Numbers of Dairy Farms

Molecular Epidemiology of Campylobacter coli Strains Isolated from Different Sources in New Zealand between 2005 and 2014

Molecular Epidemiology ofCampylobacter jejuniin a Geographically Isolated Country with a Uniquely Structured Poultry Industry

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