State of the Art in Platelet Function Testing

Kehrel, Beate E.; Brodde, Martin F.

doi:10.1159/000350469

Cited by 49 publications

(48 citation statements)

References 236 publications

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“…The latter category comprises PFA 100 and Cone and Plate(let) analyzer (R-Impact) [38]. However, it remains difficult to study platelets in vitro, given that their manipulation can induce activation [39]. Platelets are stored in a mixture of plasma and additive solution with citrate as anticoagulant, which is quite different from their physiological environment.…”

Section: Functional and Biochemical Studiesmentioning

confidence: 99%

“…More importantly, in vitro test results are often unable to predict platelet function after transfusion, because a certain degree of functional recovery may occur [42,43]. Finally, study results should be analyzed while keeping in mind the possible lack of correlation between the results and clinical efficacy [38,39,43,44].…”

Section: Functional and Biochemical Studiesmentioning

confidence: 99%

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The clinical and biological impact of new pathogen inactivation technologies on platelet concentrates

et al. 2014

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Since 1990, several techniques have been developed to photochemically inactivate pathogens in platelet concentrates, potentially leading to safer transfusion therapy. The three most common methods are amotosalen/UVA (INTERCEPT Blood System), riboflavin/UVA-UVB (MIRASOL PRT), and UVC (Theraflex-UV). We review the biology of pathogen inactivation methods, present their efficacy in reducing pathogens, discuss their impact on the functional aspects of treated platelets, and review clinical studies showing the clinical efficiency of the pathogen inactivation methods and their possible toxicity.

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Section: Functional and Biochemical Studiesmentioning

confidence: 99%

Section: Functional and Biochemical Studiesmentioning

confidence: 99%

The clinical and biological impact of new pathogen inactivation technologies on platelet concentrates

et al. 2014

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“…Platelet function tests are performed in a variety of patients, including those with bleeding disorders. In most cases, a platelet-mediated hemostatic disorder cannot be established by a single function defect; but, rather, a combination of platelet function abnormalities are required [5]. Applications of platelet function tests are listed in Box 15.1.…”

Section: Tests For Platelet Functionmentioning

confidence: 99%

Essentials of Coagulation

Wahed¹,

Dasgupta²

2015

Hematology and Coagulation

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“…Platelet assays, evaluating platelet adhesion under static or flow conditions and platelet spreading have been developed [107,150] . Platelet adhesion tests in static condition, using a large number of different surfaces -glass beads, cultured vascular cells, purified matrix proteins or complete subendothelial extracellular matrix from cultured endothelial cells -that goes to the detriment of univocal results and standardized procedure, might be achieved [151] .…”

Section: Future Perspectivesmentioning

confidence: 99%

Assessment of platelet function: Laboratory and point-of-care methods

Paniccia¹

2014

WJTM

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In the event of blood vessel damage, human platelets are promptly recruited on the site of injury and, after their adhesion, activation and aggregation, prevent blood loss with the formation of a clot. The consequence of abnormal regulation can be either hemorrhage or the development of thrombosis. Qualitative and/or quantitative defects in platelets promote bleeding, whereas the residual reactivity of platelets, despite antiplatelet therapies, play an important role in promoting arterial thrombotic complications. Platelet function is traditionally assessed to investigate the origin of a bleeding syndrome, to predict the risk of bleeding prior surgery or during pregnancy or to monitor the efficacy of antiplatelet therapy in thrombotic syndromes that, now, can be considered a new discipline. "Old" platelet function laboratory tests such as the evaluation of bleeding time and the platelet aggregation analysis in platelet-rich plasma are traditionally utilized to aid in the diagnosis and management of patients with platelet and hemostatic disorders and used as diagnostic tools both in bleeding and thrombotic diathesis in specialized laboratories. Now, new and renewed automated systems have been introduced to provide a simple, rapid assessment of platelet function including point of care methods. These new methodologies are also suitable for being used in non-specialized laboratories and in critical area for assessing platelet function in whole blood without the requirement of sample processing. Some of these methods are also beginning to be incorporated into routine clinical use and can be utilized as not only as first panel for the diagnosis of platelet dysfunction, but also for monitoring anti-platelet therapy and to potentially assess risk of both bleeding and/or thrombosis.© 2014 Baishideng Publishing Group Inc. All rights reserved.Key words: Platelets; Method; Test; Point of care testing; Laboratory assessment; Bleeding; Thrombosis; Platelet function Core tip: This review discussed the scenario of available platelet function laboratory and point-of-care methods suitable in different clinical setting. As this matter has become of crucial importance in the bleeding management and for monitoring antiplatelet therapies, improved ability to assess platelet function in a timely and efficient manner is essential. Traditional platelet function methods, requiring a fair degree of expertise, have been limited to specialized laboratory. Many efforts have been carried out for improving platelet function assays for centralized laboratory, such as different point-of-care testing methodologies have been developed. Moreover, different guidelines and recommendations for their method standardization are growing.

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State of the Art in Platelet Function Testing

Cited by 49 publications

References 236 publications

The clinical and biological impact of new pathogen inactivation technologies on platelet concentrates

The clinical and biological impact of new pathogen inactivation technologies on platelet concentrates

Essentials of Coagulation

Assessment of platelet function: Laboratory and point-of-care methods

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