Staphylococcus epidermidis Bacteriophages from the Anterior Nares of Humans

Aswani, Vijay; Tremblay, Denise M.; Moineau, Sylvain; Shukla, Sanjay Kumar

doi:10.1128/aem.05367-11

Cited by 15 publications

(11 citation statements)

References 23 publications

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“…Most of the CoNS phages belong to the Siphoviridae family, while several virulent Podoviridae phages have been recently isolated directly from human anterior nares [52]. As for S. aureus phages, the first interest brought to phage infecting these species relied on their use for clinical isolate typing [53,54].…”

Section: What About Phages In Non-aureus Staphylococci?mentioning

confidence: 99%

“…As for S. aureus phages, the first interest brought to phage infecting these species relied on their use for clinical isolate typing [53,54]. Several studies report the isolation and characterization of phages from S. epidermidis and S. saprophyticus [52,55,56,57], but only nine phages and prophages have been sequenced and studied, both at the molecular and physiological levels (Table S2). Among these, five phages and prophages are from S. epidermidis [51,58,59], one prophage from S. carnosus [48], two phages from S. hominis [37] and one phage from S. capitis [37].…”

Section: What About Phages In Non-aureus Staphylococci?mentioning

confidence: 99%

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The Staphylococci Phages Family: An Overview

Deghorain

Melderen

2012

Viruses

168

164

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Due to their crucial role in pathogenesis and virulence, phages of Staphylococcus aureus have been extensively studied. Most of them encode and disseminate potent staphylococcal virulence factors. In addition, their movements contribute to the extraordinary versatility and adaptability of this prominent pathogen by improving genome plasticity. In addition to S. aureus, phages from coagulase-negative Staphylococci (CoNS) are gaining increasing interest. Some of these species, such as S. epidermidis, cause nosocomial infections and are therefore problematic for public health. This review provides an overview of the staphylococcal phages family extended to CoNS phages. At the morphological level, all these phages characterized so far belong to the Caudovirales order and are mainly temperate Siphoviridae. At the molecular level, comparative genomics revealed an extensive mosaicism, with genes organized into functional modules that are frequently exchanged between phages. Evolutionary relationships within this family, as well as with other families, have been highlighted. All these aspects are of crucial importance for our understanding of evolution and emergence of pathogens among bacterial species such as Staphylococci.

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Section: What About Phages In Non-aureus Staphylococci?mentioning

confidence: 99%

Section: What About Phages In Non-aureus Staphylococci?mentioning

confidence: 99%

The Staphylococci Phages Family: An Overview

Deghorain

Melderen

2012

Viruses

168

164

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“…Bacteriophages are expected to be an integral part of this anterior nare ecology. In order to better understand the factors modulating nasal colonization by staphylococci, we previously isolated several Staphylococcus epidermidis phages, belonging to the Siphoviridae and Podoviridae families, from the anterior nares of humans ( 2 , 3 ). Here, we report the complete genome sequence and organization of one of the largest Siphoviridae bacteriophages isolated from the human anterior nare.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Staphylococcus epidermidis Bacteriophage Isolated from the Anterior Nares of Humans

et al. 2014

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“… 4 Free phages were detected in the nasal swabs of nearly half of the patients, which is substantially higher than previous reports. 31 , 38 This may be due to the choice of S. aureus RN4220 as the host strain, as it is negative for known RM systems and therefore susceptible to lytic phage DNA that is normally restricted. 4 It is possible a higher rate of lytic bacteriophages in the nose may be detectable with a broader range or more closely related host strains.…”

Section: Discussionmentioning

confidence: 99%

Within-host diversity of MRSA antimicrobial resistances

Stanczak-Mrozek

Manne

Knight

et al. 2015

Journal of Antimicrobial Chemotherapy

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ObjectivesMRSA is a major antimicrobial resistance (AMR) pathogen. The reservoir of infecting isolates is colonization, which is the site of evolutionary selection. The aim was to identify if AMRs in colonizing MRSA populations diversified and potential mechanisms of resistance gene transfer in vivo.MethodsNasal swabs from 38 MRSA carriers admitted to hospital were plated and 20 individual colonies from each patient tested for phenotypic antibiotic susceptibility and genetically for lineage, carriage of four prophages and three plasmid families. Free bacteriophages were detected in swabs as well as their capacity for transducing resistance genes.ResultsNine (24%) patients carried phenotypic AMR variants and 24 (63%) carried prophage and plasmid variants. If a single colony was selected for testing, the probability of detecting all AMR in that patient was 87%. Sixty-four different AMR and mobile genetic element (MGE) profiles were detected, mostly in the MRSA CC22 background (where CC stands for clonal complex), with up to 8 profiles per patient. Nearly half of the patients carried detectable free bacteriophages and phages successfully transduced resistance genes between laboratory and patient isolates in vitro. WGS showed MRSA core genomes were stable, while AMR and MGEs varied.Conclusions‘Clouds’ of MRSA variants that have acquired or lost AMR and MGEs are common in nasal colonizing populations and bacteriophages may play an important role in gene transfer. Accurate estimation of AMR and genetic variability has implications for diagnostics, epidemiology, antimicrobial stewardship and understanding the evolutionary selection of AMR in colonizing populations.

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Staphylococcus epidermidis Bacteriophages from the Anterior Nares of Humans

Cited by 15 publications

References 23 publications

The Staphylococci Phages Family: An Overview

The Staphylococci Phages Family: An Overview

Complete Genome Sequence of a Staphylococcus epidermidis Bacteriophage Isolated from the Anterior Nares of Humans

Within-host diversity of MRSA antimicrobial resistances

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