Complete Genome Sequence of a Staphylococcus epidermidis Bacteriophage Isolated from the Anterior Nares of Humans

Aswani, Vijay; Tremblay, Denise M.; Moineau, Sylvain; Shukla, Sanjay Kumar

doi:10.1128/genomea.00549-14

Cited by 13 publications

(10 citation statements)

References 8 publications

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“…This suggests that we deal with exons of the single gene (separated by the intron that contains gene for homing endonuclease). A similar layout is rather uncommon for Autographivirinae (we found it only in the genome of phage K1E) but was observed in unrelated phages, including myoviruses ( 11 – 13 ) and siphoviruses ( 14 – 17 ).…”

Section: Genome Announcementsupporting

confidence: 69%

Complete Genome Sequence of Lelliottia Podophage phD2B

et al. 2014

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Section: Genome Announcementsupporting

confidence: 69%

Complete Genome Sequence of Lelliottia Podophage phD2B

et al. 2014

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“…Three distinct types of identical repeated units of 30 or 36 nucleotides, associated with specific sequence spacers have been described (Marraffini, 2015). Three spacers that correspond to the CRISPR loci found in the strains S02, S05, and S24, match precisely with a sequence in the Staphylococcus phage PH15 genome for the first two strains and the Staphylococcus phage 6ec genome for the last (Aswani et al, 2014; Daniel, Bonnen & Fischetti, 2007).…”

Section: Resultsmentioning

confidence: 64%

Genomic diversity of prevalentStaphylococcus epidermidismultidrug-resistant strains isolated from a Children’s Hospital in México City in an eight-years survey

Cabrera-Contreras

Santamaría

Bustos

et al. 2019

PeerJ

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Staphylococcus epidermidis is a human commensal and pathogen worldwide distributed. In this work, we surveyed for multi-resistant S. epidermidis strains in eight years at a children’s health-care unit in México City. Multidrug-resistant S. epidermidis were present in all years of the study, including resistance to methicillin, beta-lactams, fluoroquinolones, and macrolides. To understand the genetic basis of antibiotic resistance and its association with virulence and gene exchange, we sequenced the genomes of 17 S. epidermidis isolates. Whole-genome nucleotide identities between all the pairs of S. epidermidis strains were about 97% to 99%. We inferred a clonal structure and eight Multilocus Sequence Types (MLSTs) in the S. epidermidis sequenced collection. The profile of virulence includes genes involved in biofilm formation and phenol-soluble modulins (PSMs). Half of the S. epidermidis analyzed lacked the ica operon for biofilm formation. Likely, they are commensal S. epidermidis strains but multi-antibiotic resistant. Uneven distribution of insertion sequences, phages, and CRISPR-Cas immunity phage systems suggest frequent horizontal gene transfer. Rates of recombination between S. epidermidis strains were more prevalent than the mutation rate and affected the whole genome. Therefore, the multidrug resistance, independently of the pathogenic traits, might explain the persistence of specific highly adapted S. epidermidis clonal lineages in nosocomial settings.

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“…Three distinct types of identical repeated units of 30 or 36 nucleotides, associated with specific sequence spacers have been described (Marraffini 2015). Three spacers that correspond to the CRISPR loci found in the strains S02, S05, and S24, match precisely with a sequence in the Staphylococcus phage PH15 genome for the first two strains and the Staphylococcus phage 6ec genome for the last (Aswani et al 2014;Daniel et al 2007).…”

Section: Survey Of Antibiotic Multidrug-resistant Intrahospital Staphmentioning

confidence: 99%

Peer Review #3 of "Genomic diversity of prevalent Staphylococcus epidermidis multidrug-resistant strains isolated from a Children’s Hospital in México City in an eight-years survey (v0.4)"

2019

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Staphylococcus epidermidis is a human commensal and pathogen worldwide distributed. In this work, we surveyed for multi-resistant S. epidermidis strains in eight years at a children's health-care unit in México City. Multidrug-resistant S. epidermidis were present in all years of the study, including resistance to methicillin, beta-lactams, fluoroquinolones, and macrolides. To understand the genetic basis of antibiotic resistance and its association with virulence and gene exchange, we sequenced the genomes of 17 S. epidermidis isolates. Whole-genome nucleotide identities between all the pairs of S. epidermidis strains were about 97% to 99%. We inferred a clonal structure and eight Multilocus Sequence Types (MLST´s) in the S. epidermidis sequenced collection. The profile of virulence includes genes involved in biofilm formation and phenol-soluble modulins (PSMs). Half of the S. epidermidis analyzed lacked the ica operon for biofilm formation. Likely, they are commensal S. epidermidis strains but multi-antibiotic resistant. Uneven distribution of insertion sequences, phages, and CRISPR-Cas immunity phage systems suggest frequent horizontal gene transfer. Rates of recombination between S. epidermidis strains were more prevalent than the mutation rate and affected the whole genome. Therefore, the multidrug resistance, independently of the pathogenic traits, might explain the persistence of specific highly adapted S. epidermidis clonal lineages in nosocomial settings.

show abstract

Complete Genome Sequence of a Staphylococcus epidermidis Bacteriophage Isolated from the Anterior Nares of Humans

Cited by 13 publications

References 8 publications

Complete Genome Sequence of Lelliottia Podophage phD2B

Complete Genome Sequence of Lelliottia Podophage phD2B

Genomic diversity of prevalentStaphylococcus epidermidismultidrug-resistant strains isolated from a Children’s Hospital in México City in an eight-years survey

Peer Review #3 of "Genomic diversity of prevalent Staphylococcus epidermidis multidrug-resistant strains isolated from a Children’s Hospital in México City in an eight-years survey (v0.4)"

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