Staphylococcus aureus isolates from dairy cows and humans on a farm differ in coagulase genotype

Molecular Analysis of Coagulase Gene Polymorphism in Clinical Isolates of Methicilin Resistant Staphylococcus aureus by Restriction Fragment Length Polymorphism Based Genotyping

“…The coagulase gene amplification has been considered a simple and accurate method for typing Staphyloccus aureus [14][15][16]25] . This method is found to be technically simple with a good reproducibility and discriminatory power.…”

“…It is now proved that coagulase is an important virulence factor during infection process. The coagulase gene amplification has been considered a simple and accurate method for typing S. aureus isolated from distinct sources [11][12][13][14][15][16] . Its discriminatory power relies on the heterogeneity of the region containing the 81 bp tandem repeats at the 3' coding region of the coagulase gene which differs both in the number of tandem repeats and the location of AluI and HaeΙΙΙ restriction sites among different isolates [17] .…”

Resistência a antimicrobianos em Staphylococcus aureus isolados de mastite em bovinos leiteiros de Minas Gerais, Brasil

“…Individualmente, S. aureus destaca-se como um dos micro-organismos mais frequentemente encontrados nas infecções intramamárias (IIM) de bovinos em todos os continentes, e também é aquele que isoladamente determina as maiores perdas na pecuária leiteira (Zschöck et al, 2000;Schlegelová et al, 2003;Vasudevan et al, 2003). No Brasil, S. aureus é considerado como o principal agente causal da mastite bovina, com taxas de isolamento entre rebanhos que variam entre 8,3% e 49,23% (Langoni et al, 1991;Costa et al, 1995;Moretti et al, 1998;Brito et al 1999;Laffranchi et al, 2001;Donatele et al, 2002).…”

Pheno- and Genotyping of Staphylococcus aureus Isolates of Sheep Origin

“…Some coa types or subtypes were recorded only in S. aureus isolates from sheep cheese (B, E and C5-C7) or sheep milk (C3, C4, D3 and D4) ( Table 4). Katsuda et al (2005) and other authors (Lange et al 1999;Schlegelová et al 2003) described a number of tandem repeats in the coa gene ranging from 3 to 9 and bovine S. aureus strains mostly showing five tandem repeats. Scherrer et al (2004) studied polymorphisms in the coa gene of S. aureus isolates from sheep and goat milk.…”