Resistência a antimicrobianos em Staphylococcus aureus isolados de mastite em bovinos leiteiros de Minas Gerais, Brasil

Costa, Geraldo Márcio da; Barros, Rodrigo Alves; Custódio, Dircéia Aparecida da Costa; Pereira, Ulisses de Pádua; Figueiredo, Demétrio Junqueira; Silva, Nivaldo da

doi:10.1590/s1808-16572013000300006

Cited by 29 publications

(32 citation statements)

References 25 publications

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“…In a large study conducted in the state of Minas Gerais, Costa et al (2013) evaluated the antimicrobial profile of 352 isolates of Staphylococcus aureus obtained from 35 different dairy properties. Resistance percentages obtained for cephalothin (0.28%), gentamicin (1.69%), neomycin (3.35%), enrofloxacin (<1.0%) and penicillin (34.1%) were very similar to our results.…”

Section: Antimicrobial Resistancementioning

confidence: 99%

Bacterial identification, somatic cell count, antimicrobial profile and toxigenic Staphylococcus strains search from mastitic cow milk samples on small farms properties

et al. 2019

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Bovine mastitis has a negative impact on milk production and can pose risks to public health. The present study aimed to evaluate the quality of bovine milk from small farms in the Botucatu/SP region. Somatic cell counts (SCC), identification of pathogens involved in mastitis, and sensitivity antimicrobial profile of staphylococci isolated were performed. The presence of enterotoxin encoding genes in isolates of staphylococci obtained from milk was investigated. Milk samples from individual mammary quarters of cows were submitted to the California mastitis test (CMT) and SCC. Of the 239 dairy cows from 21 dairy herds evaluated (mean = 11.4 animals/property), two cows (0.8%) presented clinical mastitis and 86 (35.9%) subclinical mastitis. Bacterial culture was performed in 177 quarter milk samples. Staphylococci were identified in 55 (31.1%), corynebacteria in 45 (25.4%), streptococci in 25 (14.1%) and coliforms in four (2.3%) milk samples. Average SCC from culture-positive samples was 1598x103 cells/mL, in case of staphylococci was 1362x103 cells/ml, streptococci was 2857x103 cells/mL, corynebacteria was 976x103 cells/mL and in the cases of coliforms 1161x103 cells/mL were obtained. Staphylococci showed a high sensitivity (>95%) to cephalothin, cotrimoxazole, enrofloxacin, and gentamicin, with a 41.2% resistance to penicillin and 11.8% to oxacillin. Both coagulase positive (CPS) and negative staphylococci (CNS) carried genes encoding enterotoxins in 21.6% of the first group and 41.9% in the second. The sea gene was the most detected 45.8% (n=24) between them, followed by seb with 29.2% and sec with 25.0%. The sed gene was not identified. We highlight the potential risk to public health in the possibility of strains of Staphylococcus spp. enterotoxin-producing genes that can cause staphylococcal food poisoning.

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Section: Antimicrobial Resistancementioning

confidence: 99%

Bacterial identification, somatic cell count, antimicrobial profile and toxigenic Staphylococcus strains search from mastitic cow milk samples on small farms properties

et al. 2019

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“…Bovine mastitis is considered the main disease in dairy cattle farming worldwide , Ruegg 2012, Costa et al 2013. It is an infectious process usually caused by the presence of pathogenic microorganisms in the mammary gland, affecting the production and composition of quarter milk, with reflexes mainly in somatic cell counts (SCC), total bacterial counts (TBC), and contents of casein and fat (Freitas et al 2005, Santos & Fonseca 2007, Langoni et al 2017.…”

Section: Introductionmentioning

confidence: 99%

“…INDEX TERMS: Staphylococcus aureus, Streptococcus agalactiae, mastitis, antimicrobial resistance, milk quality, dairy herds, Minas Gerais, Brazil, cattle, bacterial diseases, bacterioses, pathology. prevention of bovine mastitis (Diniz et al 2010, Jain et al 2012, Costa et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus and Streptococcus agalactiae: prevalence, resistance to antimicrobials, and their relationship with the milk quality of dairy cattle herds in Minas Gerais state, Brazil

et al. 2019

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Bovine mastitis is the most frequent disease worldwide in dairy herds, causing high economic losses to producers and industry, as well as having implications for public health due to the zoonotic potential of some agents involved in its etiology and the increased risk of antimicrobial residues in milk and its derivatives. Considering the multifactorial aspect of this disease, knowledge of the agents involved in its etiology and their antimicrobial susceptibility profiles is very important. This study was conducted with 306 dairy herds from the Campo das Vertentes region, located in the south of Minas Gerais state, whose owners were milk suppliers to a dairy in the same region. The study involved approximately 34,000 dairy cows and covered an area of approximately 12,564 km2. In these herds, prevalence rates of Staphylococcus aureus and Streptococcus agalactiae and their relationship with bulk milk somatic cell counts (BMSCC), total bacterial counts (TBC), and daily production were evaluated. In addition, analyses of resistance of these pathogens to the antimicrobials most commonly used in the treatment of mastitis in dairy herds were performed. Microbiological analyses of milk samples from collect from bulk milk tanks were performed aiming to evaluate the prevalence of S. aureus and S. agalactiae. For these proposes, the modified Baird-Parker Agar medium was used for detection of S. aureus and the modified Edwards Agar medium, enriched with 5% defibrinated sheep blood, was used for detection of S. agalactiae. The disc diffusion technique was applied to evaluate antimicrobial resistance. Results show high prevalence rates of S. aureus (70.3%) and S. agalactiae (67.0%) in the dairy farms studied, with 47.71% of the herds showing both pathogens. Associations between BMSCC and the presence of pathogens S. aureus and S. agalactiae and between TBC and the presence of S. agalactiae were observed, demonstrating the influence of these pathogens in milk quality. No variation was observed in the distribution of S. aureus and S. agalactiae in the different strata of daily production. High levels of resistance and multi-resistance were observed among the pathogens S. aureus and S. agalactiae. The results indicate the need for more effective control measures for mastitis caused by S. aureus and S. agalactiae in the dairy herds of the region studied and more judicious use of antimicrobials in order to reduce the problem of resistance to them.

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“…Também pode trazer prejuízos socioeconômicos pela transmissão de zoonoses aos contactantes e consumidores (Poletto et al, 2004). Neste sentido, uma das doenças de cunho econômico mais relevante em toda cadeia produtiva do leite é a mastite, prejuízos que vão desde a redução da produção de leite, descarte ou redução do valor comercial dos animais infectados, perdas na evolução genética do rebanho, gastos com medicamentos e mão de obra extra, até aos laticínios que tem a qualidade dos produtos derivados prejudicado pela alteração da qualidade físico-química do leite (Costa et al, 2013).…”

Section: Introductionunclassified

A prevalência de mastites em vacas leiteiras do município de Carlinda (MT), no ano de 2016

Silva

Bett

2017

Pubvet

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RESUMO.Considerando que os pequenos produtores do município de Carlinda (MT) têm como principal atividade econômica a produção de leite e, considerando que a mastite bovina se mostra como uma das causas de queda na produção do leite e desvalorização do rebanho, o objetivo deste trabalho foi o de identificar a incidência, a prevalência e as causas da ocorrência da mastite bovina na cidade de Carlinda, região norte mato-grossense. O trabalho foi realizado em 8 propriedades sendo 4 com ordenha mecânica e 4 com ordenha manual. Usou-se o CMT (Califórnia Mastitis Test). A amostra coletada foi misturada com o reagente e homogeneizada e leitura após 10 segundos. O resultado foi classificado como negativo (0), positivo que variam de traços (leve formação de gel) a fracamente positivo (+), reação positiva (++) e reação fortemente positiva (+++). Observou-se que 87,5% do rebanho de gado leiteiro do setor dessa comunidade apresentam resultado positivo para a prevalência de mastite, sendo em 100% das propriedades de ordenha manual e 75% das com ordenha mecânica. Das vacas ordenhadas manualmente, 37,5% eram positivas no teste CMT e 62,5% negativas. Das ordenhadas mecanicamente, 30% foram positivas e 70% negativas. Das propriedades estudadas, em 62,5% delas foi considerada de higiene boa e 37,5% ruim. É importante relatar que uso de pré e pós "dipping" não foi observado em nenhuma propriedade (alguns nem conhecem os procedimentos). Diante dos resultados pode se afirmar que a mastite está presente na maioria dos rebanhos leiteiros do município de Carlinda (MT) e os tipos de ordenha não apresentaram interferência na prevalência da mastite subclínica no rebanho.Palavras chave: Higiene, leite, mamite, ordenha, sanidadeThe mastitis prevalence in dairy cows in the municipality of Carlinda (Brazil), in the year of 2016 ABSTRACT. Whereas that small producers in the municipality of Carlinda (Brazil) whose main economic activity milk production and also whereas bovine mastitis is shown as one of causes of drop in milk production and impairment of the herd, the aim of this study was to identify the incidence, prevalence and causes of the occurrence of bovine mastitis in the city of Carlinda, northern Mato Grosso State (Brazil). The study was conducted in 8 properties 4 with mechanical milking and 4 with manual milking. It used the CMT (California Mastitis Test). The collected sample was mixed and homogenized with the reagent and read after 10 seconds. The result was rated as negative (0), positive ranging from traces (light gel formation) to weakly positive (+), positive reaction (++) and strong positive reaction (+++).It was observed that 87.5% of the dairy herd that community

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Resistência a antimicrobianos em Staphylococcus aureus isolados de mastite em bovinos leiteiros de Minas Gerais, Brasil

Cited by 29 publications

References 25 publications

Bacterial identification, somatic cell count, antimicrobial profile and toxigenic Staphylococcus strains search from mastitic cow milk samples on small farms properties

Bacterial identification, somatic cell count, antimicrobial profile and toxigenic Staphylococcus strains search from mastitic cow milk samples on small farms properties

Staphylococcus aureus and Streptococcus agalactiae: prevalence, resistance to antimicrobials, and their relationship with the milk quality of dairy cattle herds in Minas Gerais state, Brazil

A prevalência de mastites em vacas leiteiras do município de Carlinda (MT), no ano de 2016

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