Staphylococcus aureus adhesion to titanium oxide surfaces coated with non-functionalized and peptide-functionalized poly(l-lysine)-grafted-poly(ethylene glycol) copolymers

Multifunctional biomaterial coatings: synthetic challenges and biological activity

“…These rather small molecules can be synthesized in a tailor-made way to realize directed immobilization and multifunctionality (Delaittre et al, 2012). Thereby, immobilization anchors such as thiols (Huang et al, 2009), DOPA (Tang et al, 2014;Pagel et al, 2016), phosphonic acids (Auernheimer et al, 2005), nucleic acids (Michael et al, 2009) and basic amino acids (Harris et al, 2004;Hassert et al, 2012) are easily introduced or ligated to bioactive peptides. Moreover, additional cell-attracting or repelling functionalities can be easily incorporated by specific reactions.…”

“…The affinity of electrostatic interactions can be tuned by raising the number of charged residues as in poly-lysine (Harris et al, 2004;Hassert and Beck-Sickinger, 2013). It has been shown that the affinity of a silicon-binding peptide can be strongly increased by repeating a specific amino acid sequence, which is rich in basic amino acids (Hassert et al, 2012).…”

Evaluation of biofilm formation in the presence of saliva on poly(ethylene glycol)deposited titanium

“…Poly(ethylene glycol) (PEG) has been reported to have the ability to inhibit protein adhesion, platelet adhesion, bacterial adhesion, or biofilm formation 14,15) . In addition, PEG-immobilized Ti has been demonstrated to be effective in suppressing biofilm formation, protein adsorption, and bacterial adhesion [16][17][18] . In these studies, the methods used to immobilize PEG on metal surfaces have been complicated by the inclusion of a multistage process for the synthesis of poly( L -lysine) and PEG (PLL-g-PEG) graft copolymers and stabilization by immersion.…”

Cationic Antimicrobial Peptides

“…Various approaches have been designed in ordrer to develop such biomedical surfaces including: (i) surfaces covered with bacteria-repellent or anti-adhesive agents using highly hydrated and close-packed, chain-like molecules, such as PEG [81] or bearing negative charges [82]; (ii) polymer matrices with incorporated antibiotics (non-covalently), which are released into the surrounding medium in a controlled manner [83,84]: and (iii) antimicrobial polymers, which are either prepared by polymerization of constitutive monomers with therapeutic moieties [85−87] or covalently bound to antimicrobial agents [88−93] (Fig. 11).…”