Stanniocalcin 1 Enhances Carbon Flux from Glucose to Lipids in White Retroperitoneal Adipose Tissue in the Fed Rat

Abstract

The present work assesses in vitro the role of human Stanniocalcin 1 (hSTC-1) in glucose metabolism in white retroperitoneal adipose tissue (WRAT) from fed rat. In the fed state, hSTC1 increases the incorporation of C from glucose into lipids in the rat WRAT. The increase in lipogenesis capacity supports the hypothesis that the activity of the glycerol-3-phosphate-generating pathway (glycolysis) from glucose is regulated by hSTC-1. The effect of hSTC-1 on de novo fatty acid synthesis and on glucose oxidation in WRAT is supported by an 85 % increase inCO production from C-glucose. The incubation of WRAT in the presence of hSTC-1 maintained the ADP/ATP ratio close to the control group. The presence of hSTC-1 in the incubation medium did not inhibit the lipolytic effect of epinephrine. In conclusion, hSTC-1 is one of the hormonal factors that control glucose metabolism in WRAT in the fed state.

STC1 and PTHrP Modify Carbohydrate and Lipid Metabolism in Liver of a Teleost Fish

“…This hypothesis is supported by the fact that TMA can be converted to TMAO, which in turn inhibits the β-oxidation of lipids and promotes lipid storage 68,69 . Consistent with our results, it has been recently shown in vitro studies with fed rats that STC1 increases lipogenesis 23 . This will be an important area to explore in future studies.…”

“…However, STC binding is widespread in tissue, including mitochondria membranes of hepatocytes, myocytes and nephron cells, which together with the reported electron-transport chain stimulation suggests a role in cellular energy metabolism 20,21 . This is supported by recent in vitro studies which demonstrated STC1 modulation of renal gluconeogenesis in rat and fish 22 and lipogenesis in rat white retroperitoneal adipose tissue 23 .…”