Effects of Stanniocalcin-1 on glucose flux in rat brown adipose tissue

Cozer, Aline G.; Trapp, Márcia; Martins, Tiago Leal; Fraga, Luciano Stürmer de; Marques, Camila Moraes; Model, Jorge Felipe Argenta; Schein, Vanessa; Kucharski, Luiz Carlos; Silva, Roselis S.M. da

doi:10.1016/j.biochi.2017.04.008

Cited by 10 publications

(3 citation statements)

References 39 publications

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“…The glycoprotein stanniocalcin‐1 (STC‐1) discovered in 1995 is expressed in nerve cells, fat cells, megakaryocytes, and even cancer cells . STC‐1 is overexpressed in brain nerve cells and exerts protective effects against cellular hypoxic damage .…”

Section: Introductionmentioning

confidence: 99%

“…The glycoprotein stanniocalcin-1 (STC-1) discovered in 1995 is expressed in nerve cells, fat cells, megakaryocytes, and even cancer cells. [20][21][22] STC-1 is overexpressed in brain nerve cells and exerts protective effects against cellular hypoxic damage. 20,23 In a mouse model, STC-1 provided protection against renal ischaemia-reperfusion injury by regulating oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Oleanolic acid alleviates oxidative stress in Alzheimer’s disease by regulating stanniocalcin‐1 and uncoupling protein‐2 signalling

Guo

Zhang

et al. 2020

Clin Exp Pharma Physio

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Oxidative stress is thought to play an important role in the occurrence and development of Alzheimer's disease (AD) and antioxidants may delay or even treat AD. Oleanolic acid (OA) exhibits antioxidant properties against many diseases. However, its effects on oxidative stress in AD remain unclear. Here, we explored the role and mechanism of action of OA in N2a/APP695swe cells exposed to oxidative stress. The cells were incubated with different concentrations of OA (0, 5, 8, 10, 15, and 25 μmol/L) for 24 hours. Higher concentrations of OA (10, 15, and 25 μmol/L) significantly suppressed the apoptosis, caspase‐3 activity, reactive oxygen species level, and β amyloid (Aβ) content and increased the viability of these cells. OA (10 μmol/L) also increased the expression of stanniocalcin‐1 (STC‐1) and uncoupling protein‐2 (UCP2) in N2a/APP695swe cells. STC‐1 interference markedly reversed the effect of OA on UCP2, indicating that OA may regulate UCP2 expression in N2a/APP695swe cells via STC‐1. Moreover, UCP2 inhibition significantly reversed the OA‐mediated effects on cell viability, caspase‐3 activity, reactive oxygen species, and Aβ level. Thus, OA regulates UCP2 expression via STC‐1 to alleviate oxidative stress and Aβ level in N2a/APP695swe cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oleanolic acid alleviates oxidative stress in Alzheimer’s disease by regulating stanniocalcin‐1 and uncoupling protein‐2 signalling

Guo

Zhang

et al. 2020

Clin Exp Pharma Physio

View full text Add to dashboard Cite

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“…Besides being enriched in Agrp neurons in the Arc [77], nothing is known about the function of Acvr1c in the hypothalamus. Secreted proteins The secreted protein Stc1 codes for Stanniocalcin 1, involved in lipid synthesis in brown adipose tissue [149] and its mRNA is expressed very weakly in the LH and DMH in the hypothalamus (ABA). Another secreted protein, Cthrc1 (collagen triple-helix repeat-containing 1), shows expression in the LH (ABA) as well as in the PVN and SON and it regulates lipid storage and cellular glycogen levels [150].…”

Section: Novel Markers Of Lepr Lh Neuronsmentioning

confidence: 99%

Molecular profiling and targeting of brain cells and circuits related to energy balance and food reward

Georgiadou¹

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In the great, grand scheme of things, we're just tiny specks that will one day be forgotten. So it doesn't matter what we did in the past or how we'll be remembered. The only thing that matters is right now, this moment. This one spectacular moment we are sharing together. Molecular profiling and genetic targeting of brain cells RNA sequencing for molecular profiling and classification of brain cells A popular and reliable method to investigate brain heterogeneity is RNA sequencing, which provides information about the whole transcriptome and can be applied to a brain region, a specific cell population or at the single cell level. Many different cell dissociation protocols and sequencing technologies exist, the application of which can vary depending on the brain region, cell abundance, developmental stage and the desired sequencing depth. For example, when researchers desire to sequence a particular cell type, they can use a transgenic animal expressing a fluorescent protein in the cell population of interest, Targeting neural subtypes and circuits with viral vectors Molecular profiling of cells is very useful for identifying novel cell types and gaining insights into their molecular makeup and transcriptional responses to cues. Nevertheless, in order to understand cell types better, we need to gain genetic access to them. A popular method for gene delivery into the brain in order to map cells, visualize brain connectivity and study the function of cell populations and circuits is viral vector technology. Viral vectors are used to target genetic information into cells of choice. To this end, either wild-type or engineered viral capsids are used to "package" the genetic information (transgene) designed in a way to facilitate proper expression of RNA or protein. Few of the most commonly used viral vectors in neuroscience are the adeno-associated virus (AAV), lentivirus (LV), canine adenovirus 2 (Cav2) and rabies. The two former are mostly used for anterograde targeting, while the two latter are used for retrograde targeting of neurons and are extremely useful for brain mapping of circuits. In this thesis we use AAV and Cav2, therefore we will discuss those. Adeno-associated viral vectors (AAVs) AAV is the most popular viral vector system used in neuroscience and gene therapy. The first gene therapy drug, which treats retinal dystrophy, was approved in 2017, while there are at least 20 approved gene therapy treatments available and at least 4000 ongoing clinical trials [25], [26]. Besides the fact that the AAV capsid has limited capacity and can accommodate only up to 5.2 kb of DNA, their use has a lot of advantages. Genes delivered by AAVs do not incorporate into the host genome and rather stay episomal [27], therefore they do not cause insertional mutations that could lead to toxic consequences. AAV capsids Hypothalamus, energy balance and leptin In this thesis we mostly focus on cells located in the hypothalamus (Chapters 1-3) and profile leptin receptor-expressing cells (Chapters 1 & 2). The hypothalamus is loc...

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Effects of stanniocalcin 1 hormone on lactate metabolism in rat kidney under fed and fasted conditions

et al. 2020

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Effects of Stanniocalcin-1 on glucose flux in rat brown adipose tissue

Cited by 10 publications

References 39 publications

Oleanolic acid alleviates oxidative stress in Alzheimer’s disease by regulating stanniocalcin‐1 and uncoupling protein‐2 signalling

Oleanolic acid alleviates oxidative stress in Alzheimer’s disease by regulating stanniocalcin‐1 and uncoupling protein‐2 signalling

Molecular profiling and targeting of brain cells and circuits related to energy balance and food reward

Effects of stanniocalcin 1 hormone on lactate metabolism in rat kidney under fed and fasted conditions

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