Metabolic effects of epinephrine on the crab Neohelice granulata

Model, Jorge Felipe Argenta; Santos, Jordana Tres dos; Silva, Roselis Silveira Martins da; Vinagre, Anapaula Sommer

doi:10.1016/j.cbpa.2019.01.028

Cited by 9 publications

(7 citation statements)

References 55 publications

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“…In this study, the level of haemolymph glucose increased after ammonia-N exposure for 3 h. Similarly, it was found that the haemolymph glucose of shrimp increased significantly after acute handling stress at 1 and 2 h (30) . In Neohelice granulata, the content of glucose in haemolymph also increased significantly after 1 h salinity stress (49) . Moreover, the results of pre-experiment showed that in the normal seawater, the interference efficiency of dsCHH was 69•2 % at 24 h, but no significant changes in the expressions of other related genes were detected.…”

Section: Discussionmentioning

confidence: 91%

Effects of crustacean hyperglycaemic hormone RNA interference on regulation of glucose metabolism in Litopenaeus vannamei after ammonia-nitrogen exposure

et al. 2021

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To unveil the adaptation of Litopenaeus vannamei to elevated ambient ammonia-N, crustacean hyperglycemic hormone (CHH) was knocked down to investigate its function in glucose metabolism pathway under ammonia-N exposure. When CHH was silenced, haemolymph glucose increased significantly during 3-6 h, decreased significantly during 12-48 h, and recovered to the control groups’ level at 72 h. After CHH knockdown, DA contents reduced significantly during 3-24 h, which recovered after 48 h. Besides, the expressions of GC and DA1R in the hepatopancreas decreased significantly, while DA4R increased significantly. Correspondingly, the contents of cAMP, cGMP and DAG and the expressions of PKA, PKG, AMPKα and AMPKγ were significantly downregulated, while the levels of PKC and AMPKβ were significantly upregulated. The expressions of CREB and GLUT2 decreased significantly, while GLUT1 increased significantly. Moreover, glycogen content, glycogen synthase and glycogen phosphorylase activities in hepatopancreas and muscle were significantly increased. Furthermore, the levels of key enzymes HK, PK and PFK in glycolysis, rate-limiting enzymes CS in TCA, and critical enzymes PEPCK, FBP and G6P in gluconeogenesis were significantly decreased in hepatopancreas. These results suggest that CHH affects DA, and then they affect their receptors respectively to transmit glucose metabolism signals into the hepatopancreas of L. vannamei under ammonia-N stress. CHH acts on cGMP-PKG-AMPKα-CREB pathway through GC, and CHH affects DA to influence cAMP-PKA-AMPKγ-CREB and DAG-PKC-AMPKβ-CREB pathways, thereby regulating GLUTs, inhibiting glycogen metabolism and promoting glycolysis and gluconeogenesis. This study contributes to further understand glucose metabolism mechanism of crustacean in response to environmental stress.

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Section: Discussionmentioning

confidence: 91%

Effects of crustacean hyperglycaemic hormone RNA interference on regulation of glucose metabolism in Litopenaeus vannamei after ammonia-nitrogen exposure

et al. 2021

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“…rosenbergii (Kuo & Yang, 1999). Injections of E and 5-HT also caused hyperglycemia in the crab N. granulata (Inohara et al, 2015;Model et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…These samples were homogenized with 0.9% saline in a ratio of 1: 10 wt/vol and the concentration of triglycerides was determined with an enzymatic assay kit following the manufacturer's instructions (Labtest Diagnóstica S.A., Brazil). This methodology was already used in our previous work with the burrowing crab Neohelice granulata (Model et al, 2019). The results were expressed as mg g −1 (wet weight hepatopancreas).…”

Section: Hepatopancreas Triglyceridesmentioning

confidence: 99%

The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

et al. 2023

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Ocypode quadrata, a Ghost crab species found along the western Atlantic coast, is considered a bioindicator of anthropogenic impact on sandy beaches. Ghost Crabbing, a touristic activity in which ghost crabs are chased just for fun, is a potentially threatening activity for this crab. In crustaceans, metabolites such as glucose and lactate, and the gene expression of crustacean hyperglycemic hormone (CHH) and heat shock proteins (HSPs) increase when the animals are exposed to several types of stress, including alterations in temperature, salinity, or exposure to xenobiotics. This work was developed to identify if being chased by humans would affect these markers of stress in this species of crab. The effects of chasing stress on hemolymph and tissue metabolites and the gene expression levels of CHH and HSP70 were investigated. The levels of lactate in the hemolymph of stressed crabs were six times higher than those of control crabs immediately after chasing and decreased progressively during recovery, indicating an active anaerobic metabolism during the stress. On the contrary, glucose levels in the hemolymph of the stressed crabs increased progressively from 30 to 60 min after chasing, indicating an inverse correlation between glucose and lactate and the conversion of lactate to glucose by gluconeogenesis. In stressed crabs, the levels of triglycerides in the hemolymph decreased 30 min after chasing, while the opposite tended to occur in the hepatopancreas, indicating that during recovery, the crabs use triglycerides as energy source to sustain aerobic metabolism. Finally, this study demonstrates that ghost crabs are stressed by minimum human contact and that “ghost crabbing” must not be encouraged as a tourist activity.

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“…Contaminations of pharmaceuticals and microplastics raised serious issues worldwide [49,50], the epinephrine is no exception as it is widely used for the growth promoter in livestock and anaphylaxis treatment in human [51,52]. When epinephrine is released into the environment, such as the wastewater effluents, sewage sludge that mosquito larvae may live, the epinephrine might go into the mosquito body, and further to the food chain that involves many other organisms [53,54]. Studies have indicated that the effects of different doses of epinephrine on flesh fly larvae, which can reduce the production of reactive oxygen species [55].…”

Section: Discussionmentioning

confidence: 99%

Arylalkalamine N‐acetyltransferase‐1 acts on a secondary amine in the yellow fever mosquito, Aedes aegypti

et al. 2022

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Arylalkylamine N-acetyltransferase (aaNAT) in Aedes aegypti is primarily involved in cuticle pigmentation and formation. The reported arylalkylamine substrates are all primary amines. In this study, we report a novel substrate, a secondary amine, of Ae. aegypti aaNAT1. The recombinant aaNAT1 protein exhibited high activity to a secondary amine, epinephrine, which has not been reported for any aaNATs previously. Structure-activity relationship study demonstrated that aaNAT1 has an epinephrine-binding site, and molecular docking and dynamic simulation showed that epinephrine is quite stable in the active cavity. Further functional studies demonstrated that epinephrine affected mosquito fecundity, egg hatching and development. The new biochemical function of aaNAT1 in metabolizing epinephrine could reduce some negative effects of the compound in the mosquito.

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Metabolic effects of epinephrine on the crab Neohelice granulata

Cited by 9 publications

References 55 publications

Effects of crustacean hyperglycaemic hormone RNA interference on regulation of glucose metabolism in Litopenaeus vannamei after ammonia-nitrogen exposure

Effects of crustacean hyperglycaemic hormone RNA interference on regulation of glucose metabolism in Litopenaeus vannamei after ammonia-nitrogen exposure

The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

Arylalkalamine N‐acetyltransferase‐1 acts on a secondary amine in the yellow fever mosquito, Aedes aegypti

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